Biotechnology Bulletin ›› 2022, Vol. 38 ›› Issue (2): 141-149.doi: 10.13560/j.cnki.biotech.bull.1985.2021-0241

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Effects on the Biosynthesis of Ethanol by Promoters PpetE and Pcpc560 in Synechocystis sp. PCC 6803

YE Peng-lin(), Kwasi Kyere-Yeboah, GAO E-bin()   

  1. School of Environmental and Safety Engineering,Jiangsu University,Zhenjiang 212000
  • Received:2021-03-03 Online:2022-02-26 Published:2022-03-09
  • Contact: GAO E-bin E-mail:1776455793@qq.com;gaofei@ujs.edu.cn

Abstract:

To increase the synthesized ethanol yield of engineered Synechocystis sp. PCC 6803,the strong promoter Pcpc560 was selected to drive and enhance the expression of exogenous ethanol-producing genes(pdc,and yqhD),thus ethanol yield was enhanced. The specific methods were that by homologous double exchange,the pyruvate decarboxylase gene(pdc)derived from Zymomonas mobilis and NADPH-dependent aldehyde reductase(yqhD)derived from Escherichia coli were introduced and driven by different promoters. By reverse transcription quantitative PCR analysis,the expressions of exogenous ethanol-producing genes(pdc and yqhD)and the ethanol yields of corresponding engineered strains under the driven of different promoters were detected and compared. The results showed that the light intensity promoter,Pcpc560 derived from the Synechocystis sp. PCC 6803 significantly enhanced the expressions of the exogenous ethanol-producing genes(pdc and yqhD)and increased synthesized ethanol output compared with the medium copper ion-induced promoter PpetE. The combined expression of super-strong promoter Pcpc560 with pdc and yqhD has significantly improved the ethanol production of the engineered strain.

Key words: ethanol, promoter, Synechocystis sp. PCC 6803, synthesis, reverse transcription