Biotechnology Bulletin ›› 2022, Vol. 38 ›› Issue (3): 103-112.doi: 10.13560/j.cnki.biotech.bull.1985.2021-0619

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Screening of Potential PGPR Strains Producting Growth-promoting Volatile Compounds and Study on Their Growth-promoting Characteristics

GAO Ya-hui(), JIANG Ming-guo, FENG Jing(), ZHOU Gui   

  1. Key Laboratory of Marine Biological Resources Protection and Utilization,Guangxi Key Laboratory for Polysaccharide Materials and Modifications,School of Marine Sciences and Biotechnology,Guangxi University for Nationalities,Nanning 530008
  • Received:2021-05-12 Online:2022-03-26 Published:2022-04-06
  • Contact: FENG Jing E-mail:1393901557@qq.com;fengjing0414141@163.com

Abstract:

The aim of this work is to screen the potential plant growth-promoting rhizobacteria(PGPR)whose volatile organic compounds(VOCs)can promote plant growth,and to investigate the growth-promoting effect of the VOCs on plants and other growth-promoting functions,which provides new ideas and reliable materials for the research and development of microbial fertilizers. Using 48 functional bacteria isolated from marine samples and preserved in our laboratory as test strains,two-compartment plate test and VOCs pot experiment were used to screen them,16S rRNA to identify them,and GC-MS to analyze the VOCs components produced by the strains. Finally plate activity test was applied to detect the activities of the bacteria for nitrogen fixation,phosphorus solubilization and IAA production. The results showed that a strain of potential PGPR GX14001 was obtained. The VOCs produced by PGPR-GX14001 showed obvious growth-promoting effects on both Nicotiana benthamiana and Brassica chinensis L. seedlings. The strain GX14001 was identified as Microbacterium aurantiacum by 16S rRNA. Seven specific compounds were obtained by GC-MS analysis of VOCs. The GX14001 had strong dissolved organic/inorganic phosphorus activity and general nitrogen fixation activity,but weak IAA production capacity,which was 1.737 μg/mL. PGPR promoted the growth of plants through different growth-promoting mechanisms. The results showed that the VOCs produced by GX14001 had obvious growth-promoting effect on plants,but not high in the other aspects of growth-promoting activity;and there was no high positive correlation between them,indicating that the most important growth-promoting effect was via the produced VOCs.

Key words: plant growth-promoting rhizobacteria(PGPR), volatile organic compounds(VOCs), Microbacterium aurantiacum, phosphorus solubilization