Biotechnology Bulletin ›› 2022, Vol. 38 ›› Issue (3): 31-40.doi: 10.13560/j.cnki.biotech.bull.1985.2021-0615

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Effects of Low Temperature Stress on Different Clones of Cryptomeria fortunei and Evaluation of Their Cold Resistance

CUI Jie-bing(), ZHANG Meng, ZHANG Ying-ting, XU Jin()   

  1. Key Laboratory of Forest Genetics & Biotechnology of the Ministry of Education,Nanjing Forestry University Co-Innovation Center for Sustainable Forestry in Southern China,Nanjing Forestry University College of Forestry,Nanjing Forestry University,Nanjing 210037
  • Received:2021-05-11 Online:2022-03-26 Published:2022-04-06
  • Contact: XU Jin E-mail:707166578@qq.com;xjinhsh@njfu.edu.cn

Abstract:

This study is aimed to assess the cold resistance of Cryptomeria fortunei by analyzing the difference of cold resistance among its different clones,providing theoretical basis for the introduction,cultivation and cold resistance breeding of C. fortunei. The study subjects were 10 2-year branches from different clones,which were treated at 4℃,0℃,-4℃,-8℃,-12℃,-16℃ and -20℃ for 12 h,respectively. They were then measured in terms of the contents of malondialdehyde(MDA),the activity of superoxide dismutase(SOD)and the parameters of chlorophyll fluorescence. Combined with principal component analysis and membership function calculation,comprehensive evaluation and cold resistance ranking were carried out. The results showed that:as the temperature decreased,the content of MDA fluctuated within a certain range of temperature,but generally it exhibited a tendency to increase first,then decrease,and finally increase. In contrast,the activity of SOD decreased shortly at first,and then increased and finally decreased after cold acclimation. The maximum photochemical efficiency(Fv/Fm)and the actual photosynthetic quantum yield(Y(II))of PS II in the different clones presented a similar trend,reaching their peak at 0℃ and decreased as the temperature futher decreasing. The photochemical quenching coefficient(qP)did not change significantly at the beginning of cold stress,but it decreased as the low temperature stress aggravated to below 0℃. There were significant differences in cold resistance among different clones of C. fortunei,among which 57#,68# and 32# clones were of stronger cold resistance,while 3#,25# and 42# clones were of weaker cold resistance.

Key words: Cryptomeria fortunei, low temperature stress, physiological index, principal component analysis, cold resistance evaluation