Effects of Trichoderma longibrachiatum on Maize Growth,Soil Fertility and Rhizosphere Microorganism

doi:10.13560/j.cnki.biotech.bull.1985.2021-0704

Abstract

Abstract:

In order to explore the application effect and mechanism of Trichoderma longibracteatus fertilizer in maize cultivation,3 treatments,YJ（0.01 g bacterial fertilizer per g soil）,YF（0.07% chemical fertilizer）and YD（0.01 g bacterial fertilizer matrix per g soil）,were set up by pot method. The growth,physiology,soil nutrients,soil enzyme activities and rhizosphere microbial community of maize under different treatments were measured. The results showed that YJ and YF treatments significantly promoted the growth of maize,and there was significant difference between them and YD treatment（P<0.05）,but there was no significant difference between YJ and YF treatment （P>0.05）. Compared with YD treatment,YJ and YF treatment increased maize seedling height,root length,aboveground dry weight,underground dry weight,aboveground fresh weight and underground fresh weight by 44.65% and 39.87%,75.74% and 71.25%,83.33% and 33.33%,300% and 150.00%,101.85% and 55.56%,356.67% and 130.00%,respectively. Compared with YD treatment,YJ and YF treatment improved the physiological indexes of maize,and YJ treatment had more obvious promoting effect on the physiological indexes of maize（P<0.05）. Both YJ and YF treatments increased soil nutrients and soil enzyme activities in maize rhizosphere,and YJ treatment increased more than YF treatment. The results showed that YJ treatment increased the number of fungal OTU and reduced the number of bacterial OTU in maize rhizosphere soil（P<0.05）. YF treatment increased the number of bacterial OTU in rhizosphere soil,but decreased the number of fungal OTU. In conclusion,compared with chemical fertilizer,T. longibracteatus fertilizer has a more obvious positive effect on maize growth,physiology,soil nutrients,soil enzyme activity and soil microbial community. It is expected to replace chemical fertilizer in whole or in part in the future,and reduce the consumption of inorganic chemical fertilizer while promoting maize growth.

Key words: Trichoderma longibrachiatum fertilizer, maize, growth physiological index, soil microorganism

ZHU Jing, YU Cun. Effects of Trichoderma longibrachiatum on Maize Growth,Soil Fertility and Rhizosphere Microorganism[J]. Biotechnology Bulletin, 2022, 38(4): 230-241.

Figures/Tables 11

References 27

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不同处理 Different treatment	苗高 Height /cm	根长 Root length/cm	侧根数 Lateral root number	干重Dry weight/（g·plant^-1）		鲜重Fresh weight/（g·plant^-1）
不同处理 Different treatment	苗高 Height /cm	根长 Root length/cm	侧根数 Lateral root number	地上Shoot	地下Root	地上Shoot	地下Root
YJ	24.20±0.61a	18.40±0.53a	8.67±2.08a	0.11±0.01a	0.08±0.01a	1.09±0.40a	1.37±0.14a
YF	23.40±1.73a	17.93±1.40a	7.00±2.65a	0.08±0.03a	0.05±0.01b	0.84±0.09a	0.69±0.33a
YD	16.73±1.58b	10.47±0.15b	2.67±1.15b	0.06±0.01b	0.02±0.01c	0.54±0.06b	0.30±0.11b

不同处理 Different treatment	苗高 Height /cm	根长 Root length/cm	侧根数 Lateral root number	干重Dry weight/（g·plant^-1）		鲜重Fresh weight/（g·plant^-1）
不同处理 Different treatment	苗高 Height /cm	根长 Root length/cm	侧根数 Lateral root number	地上Shoot	地下Root	地上Shoot	地下Root
YJ	24.20±0.61a	18.40±0.53a	8.67±2.08a	0.11±0.01a	0.08±0.01a	1.09±0.40a	1.37±0.14a
YF	23.40±1.73a	17.93±1.40a	7.00±2.65a	0.08±0.03a	0.05±0.01b	0.84±0.09a	0.69±0.33a
YD	16.73±1.58b	10.47±0.15b	2.67±1.15b	0.06±0.01b	0.02±0.01c	0.54±0.06b	0.30±0.11b

不同处理 Different treatment	全磷Total phosphorus/（g·kg^-1）	有效磷Available pho-sphorus /（mg·kg^-1）	全钾Total potas-sium/（g·kg^-1）	速效钾Available Pota-ssium/（mg·kg^-1）	全氮Total nitro- gen /（g·kg^-1）	水解氮Hydrolyzed nitrogen /（mg·kg^-1）
YJ	2.25±0.10a	44.94±4.67a	2.70±0.35a	4.18±0.29a	14.33±2.65a	57.87±6.47a
YF	2.07±0.18a	43.62±4.09a	2.47±0.12ab	3.98±0.25a	12.88±1.48a	54.60±7.89a
YD	1.63±1.58b	29.45±3.84b	2.08±0.56b	3.66±0.72b	7.37±0.58b	46.67±4.28b

不同处理 Different treatment	全磷Total phosphorus/（g·kg^-1）	有效磷Available pho-sphorus /（mg·kg^-1）	全钾Total potas-sium/（g·kg^-1）	速效钾Available Pota-ssium/（mg·kg^-1）	全氮Total nitro- gen /（g·kg^-1）	水解氮Hydrolyzed nitrogen /（mg·kg^-1）
YJ	2.25±0.10a	44.94±4.67a	2.70±0.35a	4.18±0.29a	14.33±2.65a	57.87±6.47a
YF	2.07±0.18a	43.62±4.09a	2.47±0.12ab	3.98±0.25a	12.88±1.48a	54.60±7.89a
YD	1.63±1.58b	29.45±3.84b	2.08±0.56b	3.66±0.72b	7.37±0.58b	46.67±4.28b

不同处理Different treatment	脲酶Urease/（mg·g^-1·d^-1）	蔗糖酶Sucrase/（mg·g^-1·d^-1）	磷酸酶Phosphatase/（mg·g^-1·d^-1）
YJ	128.00±2.88a	19.60±1.09a	2.45±0.30a
YF	99.06±4.99b	16.09±8.57a	2.43±0.21a
YD	93.40±5.44b	3.18±0.37b	2.05±0.66a