Construction of Microbial Consortium for Efficient Degradation of Corn Straw and Evaluation of Its Degradation Effect

doi:10.13560/j.cnki.biotech.bull.1985.2021-0968

Abstract

Abstract:

In response to the problems of difficult degradation and low utilization rate of corn straw,the efficient degradation microbial consortium HD composed of Bacillus subtilis WF-8,B. lincheniformis WF-11,B. cereus WS-1 and Streptomyces nogalater WF-10 was constructed,then the degradation effect was evaluated,which provided theoretical basis for the research and development of microbial compound agents of efficiently degrading corn stalk. Single factor experiment,orthogonal experiment and response surface method were combined to optimize the enzyme production medium and fermentation conditions of the microbial consortium HD. And experiments of liquid and solid fermentation were conducted to evaluate the degradation effect of the microbial consortium HD on corn straw. The results showed that the optimal carbon and nitrogen sources of microbial consortium HD were straw powder and（NH₄）₂SO₄,the optimal addition amounts were 15 and 4 g/L. And the optimal fermentation conditions were as follows：temperature 34℃,inoculating amount 5%,fermentation time 6 d and initial pH 7.2. Under this condition,the cellulase activity was 164.21 U/mL,1.9 times higher than that before optimization,which was significantly higher than that of a single strain. After inoculating the microbial consortium into liquid fermentation medium and solid fermentation medium,the degradation rate reached 47% and 63.6%,respectively;and the degradation rate was significantly higher than that of the single strain and control groups. The results indicate that the microbial consortium HD has great potential in the degradation of corn straw,which lays a foundation for the research and development of microbial consortium agents for the efficient degradation of corn straw.

Key words: microbial consortium, degradation of corn straw, activity of cellulase, optimization of fermentation conditions

WANG Xin-guang, TIAN Lei, WANG En-ze, ZHONG Cheng, TIAN Chun-jie. Construction of Microbial Consortium for Efficient Degradation of Corn Straw and Evaluation of Its Degradation Effect[J]. Biotechnology Bulletin, 2022, 38(4): 217-229.

Figures/Tables 14

References 39

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复合菌系 Microbial consortium	菌种名称 Name of strain
HA	WF-10、WF-11
HB	WF-10、WF-11、WF-8
HC	WF-10、WF-11、WS-1
HD	WF-10、WF-11、WF-8、WS-1

复合菌系 Microbial consortium	菌种名称 Name of strain
HA	WF-10、WF-11
HB	WF-10、WF-11、WF-8
HC	WF-10、WF-11、WS-1
HD	WF-10、WF-11、WF-8、WS-1

水平 Level	因素Factor
水平 Level	A碳源 Carbon source	B氮源 Nitrogen source	C碳源添加量 Content of carbon source/（g·L^-1）	D氮源添加量 Content of nitrogen source/（g·L^-1）
1	CMC-Na	酵母粉	5	1
2	纤维素	蛋白胨	10	3
3	秸秆粉	硫酸铵	12.5	4
4	淀粉	硝酸铵	15	5

水平 Level	因素Factor
水平 Level	A碳源 Carbon source	B氮源 Nitrogen source	C碳源添加量 Content of carbon source/（g·L^-1）	D氮源添加量 Content of nitrogen source/（g·L^-1）
1	CMC-Na	酵母粉	5	1
2	纤维素	蛋白胨	10	3
3	秸秆粉	硫酸铵	12.5	4
4	淀粉	硝酸铵	15	5

因素 Factor	水平 Level
因素 Factor	-1	0	+1
A温度/℃	30	35	40
B接种量/%	4	5	6
C时间/d	5	6	7
D初始pH	6	7	8