Biotechnology Bulletin ›› 2022, Vol. 38 ›› Issue (4): 79-85.doi: 10.13560/j.cnki.biotech.bull.1985.2021-1226
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REN Ying LIAN Tong ZHANG Chun-yi JIANG Ling()
Received:
2021-09-27
Online:
2022-04-26
Published:
2022-05-06
REN Ying LIAN Tong ZHANG Chun-yi JIANG Ling. Gene Cloning and Expression Characteristics of Methionine Synthase METS in Maize[J]. Biotechnology Bulletin, 2022, 38(4): 79-85.
引物名称 Primer name | 引物序列 Primer sequence(5'-3') | 用途 Function |
---|---|---|
ZmMETS1-F1 | CAGAAAGATGGCGTCCCACATTG | 基因克隆 Gene cloning |
ZmMETS1-R1 | GACGACGCCCTCCTCGGAAACG | |
ZmMETS2-F1 | GGGAGCAACACATGCAGTATGA- ATTC | |
ZmMETS2-R1 | CACATACCCCCAGAAACATAGCCC | |
ZmMETS3-F2 | CCTCAGACAGTCAGACGTCCCGTG | |
ZmMETS3-R2 | CTGTCCTGTACATACGTTGAGC- CTGG | |
METS1-QRT-F1 | GATCCAGGACACCACCCAGATC | qRT-PCR |
METS1-QRT-R1 | CTCCTCGGAAACGAAAAAGGC | |
METS2-QRT-F1 | TAAGATTTTACTGTTATATAGAA- AGATGGC | |
METS2-QRT-R1 | GGGACAATAAAGTGGTAGTTTG- TATC | |
METS3-QRT-F1 | CTCTACAAATGAAACCCCTCAGAC | |
METS3-QRT-R1 | GTATCCAACAATATGGGACGCC | |
ZmGAPDH-F | CCCTTCATCACCACGGACTAC | Internal primers for qRT-PCR |
ZmGAPDH-R | AACCTTCTTGGCACCACCCT |
Table 1 Primers used in the experiment
引物名称 Primer name | 引物序列 Primer sequence(5'-3') | 用途 Function |
---|---|---|
ZmMETS1-F1 | CAGAAAGATGGCGTCCCACATTG | 基因克隆 Gene cloning |
ZmMETS1-R1 | GACGACGCCCTCCTCGGAAACG | |
ZmMETS2-F1 | GGGAGCAACACATGCAGTATGA- ATTC | |
ZmMETS2-R1 | CACATACCCCCAGAAACATAGCCC | |
ZmMETS3-F2 | CCTCAGACAGTCAGACGTCCCGTG | |
ZmMETS3-R2 | CTGTCCTGTACATACGTTGAGC- CTGG | |
METS1-QRT-F1 | GATCCAGGACACCACCCAGATC | qRT-PCR |
METS1-QRT-R1 | CTCCTCGGAAACGAAAAAGGC | |
METS2-QRT-F1 | TAAGATTTTACTGTTATATAGAA- AGATGGC | |
METS2-QRT-R1 | GGGACAATAAAGTGGTAGTTTG- TATC | |
METS3-QRT-F1 | CTCTACAAATGAAACCCCTCAGAC | |
METS3-QRT-R1 | GTATCCAACAATATGGGACGCC | |
ZmGAPDH-F | CCCTTCATCACCACGGACTAC | Internal primers for qRT-PCR |
ZmGAPDH-R | AACCTTCTTGGCACCACCCT |
Fig. 4 Relative expressions of ZmMETS1/ZmMETS2/ZmMETS3 in different tissues The error line refers to the standard deviation,n=3;significant difference compared with root(*P<0.05;**P<0.01;***P<0.001;****P<0.0001). The same below
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