Fig.1 Phylogenetic analysis of BnaBRC1 protein

Fig.2 Motif analysis and conserved domain structures of BnaBRC1 gene A：Analysis of conserved motifs in the protein sequence of homologous copies of the BRC1 gene in B. napus,and A. thaliana. B：Prediction of the conserved domains in the homologous copies of BRC1 in B. napus and A. thaliana

Fig.3 Transcript abundances of five BnaBRC1 copies in different tissues of B. napus

Fig.4 BnaBRC1 gene structure with target sequences and schematics of binary plasmid vectors a：The BnaBRC1 gene structure includes 4 exons separated by three introns. The vertical line in the gene structure indicates the target site,and the arrow indicates the sgRNA direction. The target sequences are shown with the PAM highlighted in red and underlined. b：Schematic presentation of binary vector SBRC1a and SBRC1b.

Fig.5 Mutated alleles at the S2 and S3 target sites of BnaBRC1 mutants from T0 to T1 generation CRISPR/Cas9-induced insertions and deletions are indicated by red font and red hyphens respectively,while the PAM is underlined and highlighted in red. “A” and “C” are the wild type alleles of the target gene on A and C chromosome copy,and “a”and “c” are the mutated alleles of the target gene on A and C chromosome copy. “_” and “ +” indicate the deletion and insertion of bases,respectively

Fig.6 Phenotypes of BnaBRC1 mutants A and B：Statistical analysis of leaf numbers and plant height（cm）of BnaBRC1 mutants,respectively. C and D：Phenotypes at the vegetative and flowering stage,respectively,WT refers to wild type（AA1CC1CC2）,aacc1CC2 refers to double copy of homozygous BnaBRC1 mutant. The data and error bars represent the mean ± SD,n ≥ 10,and bar = 3 cm