Organic Phosphate-solubilizing Bacteria Screening in the Rhizosphere of Paeonia ostii and Study on Their Phosphate-solubilizing Capabilities

doi:10.13560/j.cnki.biotech.bull.1985.2021-0942

Abstract

Abstract:

Paeonia ostii as a new woody oil crop with high oil values,the deficiency of available phosphorus in the soil severely restricts its growth and development,yield and quality. Isolation and screening of highly effective phosphate-solubilizing bacteria（PSB）and playing the role of rhizosphere microorganisms is an effective way to achieve the goal of high yield and high quality of P. ostii and ensure the green development of agriculture. Seedlings of P. ostii ‘Fengdan’ growing for different years were selected as study objects,and phytin was selected as the only phosphorus（P）source in the medium. Then the method of plate dilution was employed to isolate organic phosphate-solubilizing bacteria. And finally the 16S rDNA sequencing technology was used for molecular identification of these strains,and methods of phosphate-solubilizing circle and liquid culture were applied to analyze their phosphate-solubilizing abilities. As results,14 strains of PSB were isolated from the rhizosphere of P. ostii in 1 year,3 and 4 years. Among them,there were 5 strains of Pseudomonas,5 strains of Bacillus,3 strains of Arthrobacter and one strain of Novosphingobium,respectively. The phosphate-solubilizing index and phosphate-solubilizing efficiency of 14 strains ranged in 1.6 to 9.0 and 60% to 800%,among them the phosphate-solubilizing index and phosphate-solubilizing efficiency of strain FD1-15 was the highest. The available P content in liquid culture of PSB ranged in 2.88 mg/L to 5.30 mg/L,and the mobilization ratio ranged in 26.44% to 62.13%. The strain FD3-13 isolated from the rhizosphere of P. ostii with three-year-seedlings presented the strongest phosphate-solubilizing capacity,which belonged to genus of Bacillus. The results provide a theoretical and technical support for improving oil yield and edible quality of oil peony and useful reference for development and application microbial fertilizers.

Key words: Paeonia ostii, organic phosphate-solubilizing bacteria, rhizosphere, phosphate-solubilizing capacity

SHEN Jia-jia, HOU Xiao-gai, WANG Er-qiang, WANG Fei, GUO Li-li. Organic Phosphate-solubilizing Bacteria Screening in the Rhizosphere of Paeonia ostii and Study on Their Phosphate-solubilizing Capabilities[J]. Biotechnology Bulletin, 2022, 38(6): 157-165.

Figures/Tables 6

References 37

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菌株编号 Strain No.	菌株名称 Strain name	序列号 Serial No.	相似性 Similarity/%
FD1-3	Bacillus aryabhattai	MW085004	100
FD1-15	Bacillus megaterium	MW085005	99
FD3-1	Bacillus aryabhattai	MW085006	100
FD3-5	Pseudomonas mandelii	MW085007	99
FD3-6	Arthrobacter siccitolerans	MW085008	99
FD3-7	Arthrobacter pascens	MW085009	99
FD3-13	Bacillus megaterium	MW085010	99
FD3-23	Arthrobacter nitroguajacolicus	MW085011	99
FD3-24	Pseudomonas frederiksbergensis	MW085012	99
FD4-4	Pseudomonas mandelii	MW085013	99
FD4-8	Pseudomonas jessenii	MW085014	99
FD4-13	Pseudomonas frederiksbergensis	MW085015	99
FD4-17	Novosphingobium gossypii	MW085016	99
FD4-19	Bacillus aryabhattai	MW085017	100

菌株编号 Strain No.	菌株名称 Strain name	序列号 Serial No.	相似性 Similarity/%
FD1-3	Bacillus aryabhattai	MW085004	100
FD1-15	Bacillus megaterium	MW085005	99
FD3-1	Bacillus aryabhattai	MW085006	100
FD3-5	Pseudomonas mandelii	MW085007	99
FD3-6	Arthrobacter siccitolerans	MW085008	99
FD3-7	Arthrobacter pascens	MW085009	99
FD3-13	Bacillus megaterium	MW085010	99
FD3-23	Arthrobacter nitroguajacolicus	MW085011	99
FD3-24	Pseudomonas frederiksbergensis	MW085012	99
FD4-4	Pseudomonas mandelii	MW085013	99
FD4-8	Pseudomonas jessenii	MW085014	99
FD4-13	Pseudomonas frederiksbergensis	MW085015	99
FD4-17	Novosphingobium gossypii	MW085016	99
FD4-19	Bacillus aryabhattai	MW085017	100

菌株编号 Strain No.	溶磷圈直径D Diameter of phosphate solubilization circle D/cm	菌落直径d Colony diameter d/cm	PSI	PSE /%
FD1-3	1.10	0.20	5.50	450
FD1-15	0.90	0.10	9.00	800
FD3-1	0.80	0.30	2.67	167
FD3-5	1.45	0.30	4.83	383
FD3-6	0.40	0.25	1.60	60
FD3-7	1.44	0.30	4.80	380
FD3-13	1.10	0.20	5.50	450
FD3-23	0.90	0.30	3.00	200
FD3-24	0.90	0.35	2.57	157
FD4-4	1.20	0.40	3.00	200
FD4-8	1.10	0.20	5.50	450
FD4-13	0.50	0.20	2.50	150
FD4-17	1.20	0.30	4.00	300
FD4-19	1.00	0.20	5.00	400

菌株编号 Strain No.	溶磷圈直径D Diameter of phosphate solubilization circle D/cm	菌落直径d Colony diameter d/cm	PSI	PSE /%
FD1-3	1.10	0.20	5.50	450
FD1-15	0.90	0.10	9.00	800
FD3-1	0.80	0.30	2.67	167
FD3-5	1.45	0.30	4.83	383
FD3-6	0.40	0.25	1.60	60
FD3-7	1.44	0.30	4.80	380
FD3-13	1.10	0.20	5.50	450
FD3-23	0.90	0.30	3.00	200
FD3-24	0.90	0.35	2.57	157
FD4-4	1.20	0.40	3.00	200
FD4-8	1.10	0.20	5.50	450
FD4-13	0.50	0.20	2.50	150
FD4-17	1.20	0.30	4.00	300
FD4-19	1.00	0.20	5.00	400