Diversity of Soil Protist Community in the Rhizosphere of Morus alba L. at Different Tree Ages

doi:10.13560/j.cnki.biotech.bull.1985.2021-1377

Abstract

Abstract:

Protists play a pivotal role in the soil microbiome,and it is important to study the distribution pattern of protists in the temporal and spatial dimensions and their effects on the microecology of the root system. In this study,we followed the trading space for time and selected soil around the rhizosphere of mulberry plants at different ages（10,80 and 200 years old）in the same mulberry（Morus alba L.）orchard as sample. Then we used Illumina high-throughput sequencing technology to determine the diversity of soil protists and differences in community composition and to analyze their drivers,which may provide a basis for exploring the ecological stability mechanisms of mulberry inter-rooted protists. The results showed that the dominant phylum species of mulberry native community were basically the same among different ages,mainly Chrysophyta,Kinetoplastida,Bacillariophyta,Rotaliida,Oomycetes and Petalomonadida,but the relative abundance varied among different ages in Paracercomonas,Petalomonas,Acanthamoeba,and Rhynchomonas were the genera with significant differences in relative abundance at 10a vs 80a,10a vs 200a,and 80a vs 200a years of age,respectively,indicating significant age variation in the composition and relative abundance of the soil native community at the genus level. Native community diversity,abundance and evenness were in very significantly negative correlation with chloride ion,Simpson index was significantly and negatively correlated with total salt and community cover was significantly and positively correlated with potassium ion and alkaline nitrogen content. In sum,there are common environmental preferences or potential biological interactions among the protists and fungi and bacteria in the rhizosphere of mulberry..

Key words: Morus alba L., high throughput sequencing, rhizosphere protist, diversity, community structure

WANG Zi-ye, WANG Zhi-gang, YAN Ai-hua. Diversity of Soil Protist Community in the Rhizosphere of Morus alba L. at Different Tree Ages[J]. Biotechnology Bulletin, 2022, 38(8): 206-215.

Figures/Tables 8

Table 1 Soil protist data statistics and alpha diversity

树龄 Tree age/a	有效序列数 Number of valid sequences	OTU数 Number of OTUs	Simpson指数Simpson index	Shannon指数Shannon index	Chao指数 Chao index	Pielou_e指数 Pielou_e index	覆盖度 Coverage%
10	315 901	2 135	0.966	6.620	560.453	509.420	99.428
80	288 092	1 578	0.966	6.346	416.783	395.180	99.692
200	355 357	284	0.933	5.334	303.817	285.500	99.740

Fig. 1 Principal component analysis（PCA）of rhizosph-ere soil protist Each dot represents a sample,and dots of different colors indicate different groups

Fig. 2 Statistics at phylum level The numbers in the outer circle are species richness,the inner circle numbers are for different age groups,the same below

Fig. 3 Statistics at genus level

Fig. 4 Number of unique and common protist species

Fig. 5 Significance analysis of differences in soil physical and chemical indicators The significance level of the mean difference is 0.05. AN is alkaline decomposition nitrogen（mg/kg）,AK is potassium ion（mg/kg）,Cl is chloride ion（mg/kg）,AP is availabfe phosphorus（mg/kg）,SOM is organic matter（mg/kg）,Na is sodium ion（mg/kg）,and TDS is total salt（mg/kg）

Table 2 Correlation analysis between soil physical and chemical properties and biodiversity indexes of soil protist

	Tree age	AK	AP	AN	SOM	TDS	Na	Cl	pH
Chao1	-0.737**	-0.374	0.092	-0.502	-0.077	-0.265	0.126	0.387	0.280
Simpson	-0.858**	0.115	0.238	-0.215	-0.091	-.538*	0.163	0.695**	-0.161
Shannon	-0.808**	-0.021	0.146	-0.311	-0.242	-0.469	0.218	0.643**	0.030
Pielou_e	-0.732**	0.262	0.193	-0.118	-0.270	-0.512	0.225	0.702**	-0.171
Goods_coverage	0.599*	0.556*	-0.035	0.572*	0.022	0.023	-0.179	-0.158	-0.416

Fig. 6 Correlation diagram of protis,fungi and bacteria network The inter-node linkage（edge）indicates that there is a correlation between the two nodes being connected,with the orange line indicating a positive correlation and the green line indicating a negative correlation

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