Effects of Soil Types on Bacterial Community Diversity on the Rhizosphere Soil of Arachis hypogaea and Yield

doi:10.13560/j.cnki.biotech.bull.1985.2021-0912

Abstract

Abstract:

The objectives of this work are to understand the relationship between bacterial community diversity of the rhizosphere soil and the yield of peanut（Arachis hypogaea）in different soil types,and to define the regional dominance of peanut production characteristics in various soil types. Using 6 soil types from the main peanut-producing areas,pot experiments were conducted to study the bacterial community structure and changing characteristics on the rhizosphere soil and non-rhizosphere soil through high-throughput sequencing technology. As results,bacterial community diversity and richness of the six soil samples showed that the bacterial richness and diversity of Shaoyang red soil of Hunan province were lower,while those of Luanxian brown soil of Hebei province were relatively higher. Actinobacteria,Proteobacteria,Chloroflexi,Acidobacteria,and Gemmatimonadetes were the common dominant phyla,whereas significant differences of the bacterial abundance were found in different soil types,among which Chlorobacteria was the most abundant in Shaoyang red soil,while Actinobacteria and Proteobacteria were enriched in the other soil types. Moreover,bacterial abundances in the rhizosphere and non-rhizosphere soils were different,and the abundances of all bacteria（including Actinobacteria,Proteobacteria,Chlorobacteria,Acidobacteria,Gemmatimonadetes,and Firmicutes）in Shaoyang red soil of Hunan province varied more than that in other areas. Redundancy analysis showed that soil physical and chemical factors of different soil types such as soil acidity（pH）,organic matter（ORM）,available nitrogen（N）,available phosphorus（P）,and available potassium（K）had different influences and positive and negative correlation with bacterial community abundance and species abundance. There was a significantly positive correlation between the bacterial community and soil factors in Jiaozuo tide brown soil of Henan province,and a significantly negative correlation between the bacterial community and soil factors in Shaoyang red soil of Hunan province. Statistical analysis demonstrated that the pod yields of Gongzhuling black soil in Jilin province,of Shihezi brown calcium soil in Xinjiang province,and of Yinan brown soil in Shandong provice were higher,while the pod yield of Shaoyang red soil in Hunan province was lower. The abundance of dominant Proteobacteria in Gongzhuling black soil of Jilin province,Shihezi brown calcium soil of Xinjiang province,and Yinan brown soil of Shandong was higher than that in Shaoyang red soil of Hunan province,and Sphingomonas was also higher in Yinan brown soil of Shandong province and Gongzhuling black soil of Jilin province. It was inferred that Proteobacteria and Sphingomonas were beneficial to peanut growth and the increase of pod yield. In conclusion,although the bacterial composition of peanut rhizosphere in different soil types was similar to some extent,the differences were obvious,and the abundance of some bacteria showed unique characteristics. Proteobacteria and Sphingomonas may be beneficial phylum and genus contributed to the increase of peanut pod yield,respectively. The peanut pod yield in Shaoyang red soil of Hunan province was the lowest,and the soil endowment advantage of peanut production was lower.

Key words: Arachis hypogaea, rhizosphere, the main peanut-producing areas, soil type, diversity of bacterial community, 16S rRNA

XU Yang, ZHANG Guan-chu, DING Hong, QIN Fei-fei, ZHANG Zhi-meng, DAI Liang-xiang. Effects of Soil Types on Bacterial Community Diversity on the Rhizosphere Soil of Arachis hypogaea and Yield[J]. Biotechnology Bulletin, 2022, 38(6): 221-234.

Figures/Tables 9

Table 1 Different soils latitude and longitude,annual average temperature,and annual mean precipitation

土壤类型 Soil type	纬度 Latitude	经度 Longitude	年平均气温 Annual average temperature/℃	年平均降水量 Annual mean precipitation/ mm
河南焦作潮褐土	34°32'04″-34°45'07″	112°16'36″-112°37'20″	12.8-14.8	528-800
山东沂南褐土	35°18'26″-35°46'15″	118°6'36″-118°43'31″	12.8-14.0	900
河北滦县褐土	39°34'52″-39°58'27″	118°14'06″-118°49'34″	12.5	697.2
吉林公主岭黑土	43°11'08″-44°09'32″	124°02'09″-125°18'22″	5.6	594.8
新疆石河子棕钙土	43°26'26″-45°20'17″	84°58'32″-86°24'04″	7.5-8.2	180-270
湖南邵阳红壤	26°40'11″-27°6'23″	110°59'02″-110°40'12″	16.1-17.1	1 300

Table 2 Analysis of nutrient components and physicochemical factors in different soil types

土壤类型 Soil type	土壤有机质 Soil organic matter（ORM）/（g·kg^-1）	碱解氮 Alkali-hydrolyzable nitrogen（N）/（mg·kg^-1）	速效磷 Available phosphorous（P）/（mg·kg^-1）	速效钾 Available potassium （K）/（mg·kg^-1）	pH
河南焦作潮褐土	14.46	92.00	17.90	102.20	7.2
山东沂南褐土	13.10	92.37	27.75	95.98	6.3
河北滦县褐土	14.62	71.00	19.32	104.00	7.15
吉林公主岭黑土	26.30	133.00	15.10	116.20	6.9
新疆石河子棕钙土	18.10	51.23	18.32	126.30	8.12
湖南邵阳红壤	12.47	67.85	6.31	72.3	4.8

Table 3 Effects of soil types on pod yield of peanut

土壤类型 Soil type	百果重 Weight of 100 pods/g	百仁重 Weight of 100 seeds/g	果产量 Pod yield/g
河南焦作潮褐土	201.31±1.44d	69.22±0.89e	285.75±3.33e
山东沂南褐土	216.90±2.69b	76.09±0.81c	354.44±3.53c
河北滦县褐土	207.93±2.10c	74.76±0.39d	310.66±1.74d
吉林公主岭黑土	233.68±3.44a	82.48±0.63a	383.80±1.50a
新疆石河子棕钙土	231.96±0.49a	81.13±0.81b	370.75±1.52b
湖南邵阳红壤	192.05±0.53e	67.31±0.13f	275.52±3.86f

Fig.1 α diversity analysis A：Rarefaction curve analysis. B：Rank abundance curve. C：Chao index analysis. D：Shannon index analysis

Fig.2 Bacterial community structure of each sample at the phylum level A：Percentage of bacterial community abundance at the phylum level. B：Kruskal-wallis H test was used to analyze bacterial community differences at the phylum level. * indicate significant differences,* P<0.05,** P<0.01,and*** P<0.001. The same below

Fig.3 Structure of bacterial community at the class,order and family level A：Percentage of bacterial community abundance on the rhizosphere of A. hypogaea at the class level. B：Percentage of bacterial community abundance on the rhizosphere of A. hypogaea at the order level. C：Percentage of bacterial community abundance on the rhizosphere of A. hypogaea at the family level

Fig.4 Bacterial community structure of each sample at the genus level A：Percentage of bacterial community abundance on the rhizosphere of A. hypogaea at the genus level;B：Kruskal-wallis H test was used to analyze bacterial community differences among multiple samples at the genus level

Fig.5 β diversity analysis of bacterial communities on the rhizosphere of A. hypogaea A：Principal co-ordinates analysis（PCoA）. B：Sample hierarchical clustering, 1-3：3 repeats. C：ANOSIM analysis

Fig.6 Correlation analysis between soil bacterial commu-nity and soil physical and chemical factors A：Spearman correlation heatmap. B：RDA analysis of soil bacterial community structure and soil physical and chemical factors

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