Effects of Temperature on the Growth,Total Lipid and Eicosapentaenoic Acid Synthesis of Eustigmatos sp.

doi:10.13560/j.cnki.biotech.bull.1985.2021-1293

Abstract

Abstract:

Eustigmatos sp. has received great attention due to its high content of eicosapentaenoic acid（EPA）. Temperature is an important factor affecting the synthesis of polyunsaturated fatty acids. In this study,Eustigmatos sp. JHsu-01 was used as the experimental strain and cultured in the 2 conditions,high temperature（30℃）and low temperature（15℃）. The growth,lipid accumulation,fatty acid composition and the expressions of key genes involved in lipid synthesis were measured and the effects of temperature on the EPA synthesis patterns were investigated. The results showed that the low-temperature enhanced the synthesis of membrane lipids and EPA in Eustigmatos sp.,and the highest EPA content was 2.78% DW. Glycolipids were the main carrier of EPA,but temperature changed the distribution of EPA between glycolipids and neutral lipids. The results of transcriptome demonstrated that under low temperature conditions,the expression of key genes in de novo fatty acid synthesis,triacylglycerols（GPAT,plsC,PLPP and DGAT）,glycolipids（MGD and DGD）,sulfolipids（SQD1 and SQD2）and ω-3 synthesis pathways（Δ5 Des,Δ6 Des and Δ15 Des）were up-regulated. In conclusion,low temperature is an important culture condition to promote the synthesis of EPA in Eustigmatos,and it is also an optimal cultivation approach for obtaining high-content glycolipid EPA. The research results may provide a theoretical and technical guidance to increase the production of EPA.

Key words: polyunsaturated fatty acids, Eustigmatos, EPA, temperature, transcriptome

XU Jin, LI Tao, LI Chu-lin, ZHU Shun-ni, WANG Zhong-ming, XIANG Wen-zhou. Effects of Temperature on the Growth,Total Lipid and Eicosapentaenoic Acid Synthesis of Eustigmatos sp.[J]. Biotechnology Bulletin, 2022, 38(6): 261-271.

Figures/Tables 8

References 30

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	基因功能Gene function	简写Abbreviation	变化情况Changes
脂肪酸合成 Fatty acid synthesis	乙酰辅酶A羧化酶羧基转移酶Acetyl-CoA carboxylase carboxyl transferase	accA	上调 Up
	3-酮酰基-ACP合成酶 3-oxoacyl-ACP synthase	fabF	上调 Up
	3-酮酰基-ACP还原酶 3-oxoacyl-ACP reductase	fabG	上调 Up
	3-酮酰基-ACP脱水酶 3-hydroxyacyl- ACP dehydratase	fabZ	上调 Up
	烯酯酰ACP还原酶I Enoyl-ACP reductase I	fabI	上调 Up
脂肪酸去饱和 Fatty acid desaturation	delta-5脂肪酸去饱和酶 Delta-5 fatty acid desaturase	Δ5 Des	上调 Up
	delta-6脂肪酸去饱和酶 Delta-6 fatty acid desaturase	Δ6 Des	上调 Up
	delta-15脂肪酸去饱和酶 Delta-15 fatty acid desaturase	Δ15 Des	上调 Up
三酰甘油合成 Triacylglycerol synthesis	3-磷酸甘油酰基转移酶 Glycerol-3-phosphate O-acyltransferase	GPAT	上调 Up
	甘油磷酸脱氢酶 Glycerophosphatedehydrogenase	GPDH	无变化 No
	1-酰基甘油-3-磷酸酰基转移酶 1-acyl-glycerol-3-phosphate acyltransferase	plsC	上调 Up
	磷脂酸磷酸酶 Phosphatidate phosphatase	PLPP	上调 Up
	二酰基甘油酰基转移酶 Diacylglycerol O-acyltransferase	DGAT	上调 Up
硫脂合成 Sulfolipid synthesis	硫代异鼠基转移酶 Sulfoquinovosyltransferase	SQD2	上调 Up
硫脂合成 Sulfolipid synthesis	UDP-硫代异鼠糖合酶 UDP-sulfoquinovose synthase	SQD1	上调 Up
糖脂合成 Glycolipid synthesis	二酰基甘油3-β-半乳糖基转移酶 1,2-diacylglycerol 3-beta-galactosyltransferase	MGD	上调 Up
糖脂合成 Glycolipid synthesis	二半乳糖二酰基甘油合酶 Digalactosyldiacylglycerol synthase	DGD	上调 Up
磷脂合成 Phospholipid synthesis	磷脂酰胞苷转移酶 Phosphatidate cytidylyltransferase	CDS1	无变化 No
	乙醇胺磷酸转移酶 Ethanolaminephosphotransferase	EPT1	下调 Down
	二酰基甘油胆碱磷酸转移酶 Diacylglycerol cholinephosphotransferase	CPT1	下调 Down
	磷脂酰丝氨酸合酶2 phosphatidylserine synthase 2	PTDSS2	下调 Down
	CDP-二酰基甘油-甘油-3-磷酸磷脂酰转移酶 CDP-diacylglycerol-glycerol-3-phosphate 3-phosphatidyltransferase	pgsA	无变化 No
	磷脂酰甘油磷酸酶 Phosphatidylglycerophosphatase	GEP4	无变化 No
	CDP-二酰基甘油-肌醇3-磷脂酰转移酶 CDP-diacylglycerol-inositol 3-phosphatidyltransferase	CDIPT	上调 Up

	基因功能Gene function	简写Abbreviation	变化情况Changes
脂肪酸合成 Fatty acid synthesis	乙酰辅酶A羧化酶羧基转移酶Acetyl-CoA carboxylase carboxyl transferase	accA	上调 Up
	3-酮酰基-ACP合成酶 3-oxoacyl-ACP synthase	fabF	上调 Up
	3-酮酰基-ACP还原酶 3-oxoacyl-ACP reductase	fabG	上调 Up
	3-酮酰基-ACP脱水酶 3-hydroxyacyl- ACP dehydratase	fabZ	上调 Up
	烯酯酰ACP还原酶I Enoyl-ACP reductase I	fabI	上调 Up
脂肪酸去饱和 Fatty acid desaturation	delta-5脂肪酸去饱和酶 Delta-5 fatty acid desaturase	Δ5 Des	上调 Up
	delta-6脂肪酸去饱和酶 Delta-6 fatty acid desaturase	Δ6 Des	上调 Up
	delta-15脂肪酸去饱和酶 Delta-15 fatty acid desaturase	Δ15 Des	上调 Up
三酰甘油合成 Triacylglycerol synthesis	3-磷酸甘油酰基转移酶 Glycerol-3-phosphate O-acyltransferase	GPAT	上调 Up
	甘油磷酸脱氢酶 Glycerophosphatedehydrogenase	GPDH	无变化 No
	1-酰基甘油-3-磷酸酰基转移酶 1-acyl-glycerol-3-phosphate acyltransferase	plsC	上调 Up
	磷脂酸磷酸酶 Phosphatidate phosphatase	PLPP	上调 Up
	二酰基甘油酰基转移酶 Diacylglycerol O-acyltransferase	DGAT	上调 Up
硫脂合成 Sulfolipid synthesis	硫代异鼠基转移酶 Sulfoquinovosyltransferase	SQD2	上调 Up
硫脂合成 Sulfolipid synthesis	UDP-硫代异鼠糖合酶 UDP-sulfoquinovose synthase	SQD1	上调 Up
糖脂合成 Glycolipid synthesis	二酰基甘油3-β-半乳糖基转移酶 1,2-diacylglycerol 3-beta-galactosyltransferase	MGD	上调 Up
糖脂合成 Glycolipid synthesis	二半乳糖二酰基甘油合酶 Digalactosyldiacylglycerol synthase	DGD	上调 Up
磷脂合成 Phospholipid synthesis	磷脂酰胞苷转移酶 Phosphatidate cytidylyltransferase	CDS1	无变化 No
	乙醇胺磷酸转移酶 Ethanolaminephosphotransferase	EPT1	下调 Down
	二酰基甘油胆碱磷酸转移酶 Diacylglycerol cholinephosphotransferase	CPT1	下调 Down
	磷脂酰丝氨酸合酶2 phosphatidylserine synthase 2	PTDSS2	下调 Down
	CDP-二酰基甘油-甘油-3-磷酸磷脂酰转移酶 CDP-diacylglycerol-glycerol-3-phosphate 3-phosphatidyltransferase	pgsA	无变化 No
	磷脂酰甘油磷酸酶 Phosphatidylglycerophosphatase	GEP4	无变化 No
	CDP-二酰基甘油-肌醇3-磷脂酰转移酶 CDP-diacylglycerol-inositol 3-phosphatidyltransferase	CDIPT	上调 Up