Biotechnology Bulletin ›› 2022, Vol. 38 ›› Issue (6): 93-102.doi: 10.13560/j.cnki.biotech.bull.1985.2021-1058
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NIE Li-bin1,2(
), YI Ling-xin1,2, DENG Yan1,2, SHENG Qi1,2, WU Xiao-yu1,2, ZHANG Bin1,2()
Received:2021-08-18
Online:2022-06-26
Published:2022-07-11
Contact:
ZHANG Bin
E-mail:857693610@qq.com;zhangbin2919@jxau.edu.cn
NIE Li-bin, YI Ling-xin, DENG Yan, SHENG Qi, WU Xiao-yu, ZHANG Bin. Pathway Engineering Modification of Corynebacterium glutamicum for Shikimic Acid Production[J]. Biotechnology Bulletin, 2022, 38(6): 93-102.
| Strain | Relevant genotype | Reference |
|---|---|---|
| Escherichia coli DH5α | Cloning host | Lab stock |
| Corynebacterium. glutamicum CICC 20189 | Wild type,L-phenylalanine producing strain | Purchased from CICC |
| SKA01 | CICC 20189 with deletion of aroK | This study |
| SKA02 | CICC 20189 with deletion of aroK and a strong PNCgl0824 promoter insertion in the upstream region of aroG | This study |
| SKA03 | CICC 20189 with deletion of aroK and a strong Psod promoter insertion in the upstream region of aroG | This study |
| SKA04 | CICC 20189 with deletion of aroK,a strong PNCgl0824 promoter insertion in the upstream region of aroG,and deletion of qsuB | This study |
| SKA05 | CICC 20189 with deletion of aroK,a strong PNCgl0824 promoter insertion in the upstream region of aroG,deletion of qsuB,and insertion of SaroGE.coli at the △qsuB site. | This study |
| SKA06 | CICC 20189 with deletion of aroK,a strong PNCgl0824 promoter insertion in the upstream region of aroG,deletion of qsuB,and insertion of ParoGE.coli at the △qsuB site. | This study |
Table 1 Corynebacterium glutamicum and Escherichia coli strains used in this study
| Strain | Relevant genotype | Reference |
|---|---|---|
| Escherichia coli DH5α | Cloning host | Lab stock |
| Corynebacterium. glutamicum CICC 20189 | Wild type,L-phenylalanine producing strain | Purchased from CICC |
| SKA01 | CICC 20189 with deletion of aroK | This study |
| SKA02 | CICC 20189 with deletion of aroK and a strong PNCgl0824 promoter insertion in the upstream region of aroG | This study |
| SKA03 | CICC 20189 with deletion of aroK and a strong Psod promoter insertion in the upstream region of aroG | This study |
| SKA04 | CICC 20189 with deletion of aroK,a strong PNCgl0824 promoter insertion in the upstream region of aroG,and deletion of qsuB | This study |
| SKA05 | CICC 20189 with deletion of aroK,a strong PNCgl0824 promoter insertion in the upstream region of aroG,deletion of qsuB,and insertion of SaroGE.coli at the △qsuB site. | This study |
| SKA06 | CICC 20189 with deletion of aroK,a strong PNCgl0824 promoter insertion in the upstream region of aroG,deletion of qsuB,and insertion of ParoGE.coli at the △qsuB site. | This study |
| Plasmid | Characteristics | Reference |
|---|---|---|
| pK18mobsacB | Mobilizable vector,allows for selection of double crossover in C. glutamicum,KmR,sacB | Lab stock |
| pK18-△aroK | A derivative of pK18mobsacB,harboring △aroK fragment | This study |
| pK18-ParoG | A derivative of pK18mobsacB,harboring ParoG fragment | This study |
| pK18-SaroG | A derivative of pK18mobsacB,harboring SaroG fragment | This study |
| pK18-△qsuB | A derivative of pK18mobsacB,harboring △qsuB fragment | This study |
| pK18-△qsuB-SaroGE.coli | A derivative of pK18mobsacB,harboring △qsuB-SaroGE.coli fragment | This study |
| pK18-△qsuB-ParoGE.coli | A derivative of pK18mobsacB,harboring △qsuB-ParoGE.coli fragment | This study |
Table 2 Plasmids used in this study
| Plasmid | Characteristics | Reference |
|---|---|---|
| pK18mobsacB | Mobilizable vector,allows for selection of double crossover in C. glutamicum,KmR,sacB | Lab stock |
| pK18-△aroK | A derivative of pK18mobsacB,harboring △aroK fragment | This study |
| pK18-ParoG | A derivative of pK18mobsacB,harboring ParoG fragment | This study |
| pK18-SaroG | A derivative of pK18mobsacB,harboring SaroG fragment | This study |
| pK18-△qsuB | A derivative of pK18mobsacB,harboring △qsuB fragment | This study |
| pK18-△qsuB-SaroGE.coli | A derivative of pK18mobsacB,harboring △qsuB-SaroGE.coli fragment | This study |
| pK18-△qsuB-ParoGE.coli | A derivative of pK18mobsacB,harboring △qsuB-ParoGE.coli fragment | This study |
| Primer name | Sequence（5'-3'） | Size/bp |
|---|---|---|
| aroK-up-F | aacgacggccagtgccaagctCAATCGATGATTCCCCAGTTC | 42 |
| aroK -up-R | GAAAAGCTTGGGAAACGTCTAGAGCGTCGGAGTCGACGAGTTCAGTG | 47 |
| aroK -down-F | GCTCTAGACGTTTCCCAAGCTTTTCAAGCACTGAAATCCTCCGGAGT | 47 |
| aroK -down-R | cggtacccggggatcctctagCGGTGTCAATGAATACTGCGTC | 43 |
| aroK -check-F | AAAAGCCTGTGGCGCCGTGTT | 21 |
| aroG-up-F | aacgacggccagtgccaagctCTTGTGAGCGCTTCTTTGATC | 42 |
| aroG-up-R（sod） | CAAGCCCGGAATAATTGGCAATGGGATGGGGTGAATTTAGG | 41 |
| aroG-up-R（P） | CTATCCGTATTAGTGGCACAGTTATGGGATGGGGTGAATTTAGG | 44 |
| sod-F | TGCCAATTATTCCGGGCTTG | 20 |
| sod-R | TCCGCACCGAGCATATACATCTT | 23 |
| PNCgl0824-F | AACTGTGCCACTAATACGGATAG | 23 |
| PNCgl0824-R | CAATTTCGCCTGCTTCCGATT | 21 |
| aroG-down-F（sod） | AAGATGTATATGCTCGGTGCGGATGCATAGCCCTGAAAGGCAAG | 44 |
| aroG-down-F（P） | AATCGGAAGCAGGCGAAATTGTGCATAGCCCTGAAAGGCAAG | 42 |
| aroG-down-R | cggtacccggggatcctctagTCGTCGGAGGTTCCGAAGAAG | 42 |
| qsuB-up-F | aacgacggccagtgccaagctGCTCGCGTTGCTATTGCTG | 40 |
| qsuB-up-R | GAAAAGCTTGGGAAACGTCTAGAGCGAAACCACCAAGTCCTGCTCG | 46 |
| qsuB-down-F | GCTCTAGACGTTTCCCAAGCTTTTCATCTGTTCCAGCCGTTTCGAG | 46 |
| qsuB-down-R | cggtacccggggatcctctagCACCCAAGCGGAAACCCAATT | 42 |
| qsuB-check-F | GGCTCAGGATTTGGGATTAAC | 21 |
| Sod-F（qsuB） | ACTTGGTGGTTTCGCTCTAGATGCCAATTATTCCGGGCTTG | 41 |
| Sod-R（aroGE. coli） | AAATCGTCGTTCTGATAATTCATTCCGCACCGAGCATATACATCTT | 46 |
| P-F（qsuB） | ACTTGGTGGTTTCGCTCTAGAAACTGTGCCACTAATACGGATAG | 44 |
| P-R（aroGE. coli） | AAATCGTCGTTCTGATAATTCATCAATTTCGCCTGCTTCCGATT | 44 |
| aroGE. coli-F1 | ATGAATTATCAGAACGACGATTT | 23 |
| aroGE. coli-R1 | CGACCGGACAAAAAAGCCCTGATGCCAGTTCG | 32 |
| aroGE. coli-F2 | GCATCAGGGCTTTTTTGTCCGGTCGGCTTCAAAAATG | 37 |
| aroGE. coli-R2 | ACGGCTGGAACAGATGAAAAGCTTTTACCCGCGACGCGCTTTTACT | 46 |
Table 3 Primers used in this study
| Primer name | Sequence（5'-3'） | Size/bp |
|---|---|---|
| aroK-up-F | aacgacggccagtgccaagctCAATCGATGATTCCCCAGTTC | 42 |
| aroK -up-R | GAAAAGCTTGGGAAACGTCTAGAGCGTCGGAGTCGACGAGTTCAGTG | 47 |
| aroK -down-F | GCTCTAGACGTTTCCCAAGCTTTTCAAGCACTGAAATCCTCCGGAGT | 47 |
| aroK -down-R | cggtacccggggatcctctagCGGTGTCAATGAATACTGCGTC | 43 |
| aroK -check-F | AAAAGCCTGTGGCGCCGTGTT | 21 |
| aroG-up-F | aacgacggccagtgccaagctCTTGTGAGCGCTTCTTTGATC | 42 |
| aroG-up-R（sod） | CAAGCCCGGAATAATTGGCAATGGGATGGGGTGAATTTAGG | 41 |
| aroG-up-R（P） | CTATCCGTATTAGTGGCACAGTTATGGGATGGGGTGAATTTAGG | 44 |
| sod-F | TGCCAATTATTCCGGGCTTG | 20 |
| sod-R | TCCGCACCGAGCATATACATCTT | 23 |
| PNCgl0824-F | AACTGTGCCACTAATACGGATAG | 23 |
| PNCgl0824-R | CAATTTCGCCTGCTTCCGATT | 21 |
| aroG-down-F（sod） | AAGATGTATATGCTCGGTGCGGATGCATAGCCCTGAAAGGCAAG | 44 |
| aroG-down-F（P） | AATCGGAAGCAGGCGAAATTGTGCATAGCCCTGAAAGGCAAG | 42 |
| aroG-down-R | cggtacccggggatcctctagTCGTCGGAGGTTCCGAAGAAG | 42 |
| qsuB-up-F | aacgacggccagtgccaagctGCTCGCGTTGCTATTGCTG | 40 |
| qsuB-up-R | GAAAAGCTTGGGAAACGTCTAGAGCGAAACCACCAAGTCCTGCTCG | 46 |
| qsuB-down-F | GCTCTAGACGTTTCCCAAGCTTTTCATCTGTTCCAGCCGTTTCGAG | 46 |
| qsuB-down-R | cggtacccggggatcctctagCACCCAAGCGGAAACCCAATT | 42 |
| qsuB-check-F | GGCTCAGGATTTGGGATTAAC | 21 |
| Sod-F（qsuB） | ACTTGGTGGTTTCGCTCTAGATGCCAATTATTCCGGGCTTG | 41 |
| Sod-R（aroGE. coli） | AAATCGTCGTTCTGATAATTCATTCCGCACCGAGCATATACATCTT | 46 |
| P-F（qsuB） | ACTTGGTGGTTTCGCTCTAGAAACTGTGCCACTAATACGGATAG | 44 |
| P-R（aroGE. coli） | AAATCGTCGTTCTGATAATTCATCAATTTCGCCTGCTTCCGATT | 44 |
| aroGE. coli-F1 | ATGAATTATCAGAACGACGATTT | 23 |
| aroGE. coli-R1 | CGACCGGACAAAAAAGCCCTGATGCCAGTTCG | 32 |
| aroGE. coli-F2 | GCATCAGGGCTTTTTTGTCCGGTCGGCTTCAAAAATG | 37 |
| aroGE. coli-R2 | ACGGCTGGAACAGATGAAAAGCTTTTACCCGCGACGCGCTTTTACT | 46 |
Fig.1 Pathway of shikimic acid in C. glutamicum PEP：phosphoenolpyruvate;E4P：erythrose-4-phosphate;DAHP：3-deoxy-D-arabinoheptulosonate-7-phosphate;DHQ：3-dehydro-quinate;DHS：3-dehydroshikimate;PCA：protocatechuate;S3P：shikimate-3-phosphate SA,shikimic acid;aroG：encoding DAHP synthetase;aroGE. coli：encoding DAHP synthetase from E. coli;aroB：encoding DHQ synthase;aroD：encoding DHQ dehydratase;aroE：encoding shikimate dehydrogenase;aroK：encoding shikimate kinase;qsuB：encoding DHS dehydratase;qsuD：encoding QA/ shikimate dehydrogenase
Fig.2 Construction and fermentation evaluation of aroK-deleted strain A：PCR identification of positive strain with aroK deleted. M：DNA marker;1：amplifying C. glutamicum CICC 20189 using aroK-check-F and aroK-down-R as primers. 4：Negative control. 2,3,5,6：amplifying C. glutamicum SKA01 using aroK-check-F and aroK-down-R as primers. B：Shikimic acid production at fermented 48 h and 72 h of strain C. glutamicum CICC 20189 and SKA01.C：HPLC analysis of shikimic acid produced by different strains in contrast with standard sample
Fig.3 PCR identification and shake flask fermentation evaluation of recombinant strain C. glutamicum SKA02 and SKA03 A：PCR identification of recombinant strain SKA02. M：DNA marker;1,2：amplifying C. glutamicum SKA02 using PNCgl0824-F and aroG-down-R as primers;3：amplifying C. glutamicum SKA01 using PNCgl0824-F and aroG-down-R as primers. B：PCR identification of recombinant strain SKA03. M：DNA marker;1-5：amplifying C. glutamicum SKA03 using sod-F and aroG-down-R;6：amplifying C. glutamicum SKA01 using sod-F and aroG-down-R. C：Shikimic acid production curves of C. glutamicum strain SKA02 and SKA03. D：Cell growth curves of C. glutamicum strain SKA02 and SKA03
Fig.4 Construction and fermentation evaluation of recom-binant C. glutamicum strain SKA04 A：PCR identification of recombinant SKA04. M：DNA marker;1-3：amplifying C. glutamicum SKA04 using qsuB-check-F and qsuB-down-R as primers;4：amplifying C. glutamicum SKA02 using qsuB-check-F and qsuB-down-R as primers. B：Shikimic acid production curves of C. glutamicum strain SKA02 and SKA04. C：Cell growth curves of C. glutamicum strain SKA02 and SKA04
Fig. 5 Construction and shake flask fermentation evaluation of recombinant C. glutamicum strain SKA04,SKA05,and SKA06 A：PCR identification of SKA05. M：DNA marker;1：amplifying C. glutamicum SKA04 using PNCgl0824-F and aroGE. coli-R1 as primers;2-8：amplifying C. glutamicum SKA05 using PNCgl0824-F and aroGE. coli-R1 as primers. B：PCR identification of SKA06. M：DNA marker;1,2：amplifying C. glutamicum SKA06 using sod-F and aroGE. coli-R1 as primers;3：amplifying C. glutamicum SKA04 using sod-F and aroGE. coli-R1 as primers. C：Shikimic acid production curves of C. glutamicum strain SKA04,SKA05,and SKA06. D：Cell growth curves of C. glutamicum strain SKA04,SKA05,and SKA06
| [1] | 江晶洁, 刘涛, 林双君. 基于莽草酸途径微生物合成芳香族化合物及其衍生物的研究进展[J]. 生命科学, 2019, 31(5):430-448. |
| Jiang JJ, Liu T, Lin SJ. Research progress on the biosynthesis of aromatic compounds by microorganisms[J]. Chin Bull Life Sci, 2019, 31(5):430-448. | |
| [2] |
Gong J, Xu W. Different synthetic strategies of oseltamivir phosphate:a potent influenza neuraminidase inhibitor[J]. Curr Med Chem, 2008, 15(30):3145-3159.
doi: 10.2174/092986708786848497 URL |
| [3] |
Jiang M, Zhang H. Engineering the shikimate pathway for biosynthesis of molecules with pharmaceutical activities in E. coli[J]. Curr Opin Biotechnol, 2016, 42:1-6.
doi: 10.1016/j.copbio.2016.01.016 URL |
| [4] | Adelfo Escalante A, Carmona SB, Diaz Quiroz DC, et al. Current perspectives on applications of shikimic and aminoshikimic acids in pharmaceutical chemistry[J]. Res Rep Med Chem, 2014, 4:35-46. |
| [5] |
Gibson JM, Thomas PS, Thomas JD, et al. Benzene-free synthesis of phenol[J]. Angew Chem Int Ed, 2001, 40(10):1945-1948.
pmid: 11385681 |
| [6] |
Candeias NR, Assoah B, Simeonov SP. Production and synthetic modifications of shikimic acid[J]. Chem Rev, 2018, 118(20):10458-10550.
doi: 10.1021/acs.chemrev.8b00350 pmid: 30350584 |
| [7] |
Sui R. Separation of shikimic acid from pine needles[J]. Chem Eng Technol, 2008, 31(3):469-473.
doi: 10.1002/ceat.200700413 URL |
| [8] | 何邵平, 禹琪芳, 段杰林, 等. 莽草酸的制备及其生物学功能研究进展[J]. 饲料博览, 2014(10):32-36. |
| He SP, Yu QF, Duan JL, et al. Advance on preparation and biological function of shikimic acid[J]. Feed Rev, 2014(10):32-36. | |
| [9] |
She DJ, Singh G. Chemical synthesis of shikimic acid and its analogues[J]. Tetrahedron, 1998, 54(19):4697-4753.
doi: 10.1016/S0040-4020(98)00016-7 URL |
| [10] |
Ghosh S, Chisti Y, Banerjee UC. Production of shikimic acid[J]. Biotechnol Adv, 2012, 30(6):1425-1431.
doi: 10.1016/j.biotechadv.2012.03.001 URL |
| [11] |
Rawat G, Tripathi P, Saxena RK. Expanding horizons of shikimic acid[J]. Appl Microbiol Biotechnol, 2013, 97(10):4277-4287.
doi: 10.1007/s00253-013-4840-y URL |
| [12] | McCrindle R, Overton KH, Raphael RA. 315. A stereospecific total synthesis ofD-(-)-shikimic acid[J]. J Chem Soc, 1960:1560-1565. |
| [13] |
Box JM, Harwood LM, Humphreys JL, et al. Dehydration of quinate derivatives:synthesis of a difluoromethylene homologue of shikimic acid[J]. Synlett, 2002, 2002(2):358-360.
doi: 10.1055/s-2002-19772 URL |
| [14] |
Zhang B, Liu ZQ, Liu C, et al. Application of CRISPRi in Corynebacterium glutamicum for shikimic acid production[J]. Biotechnol Lett, 2016, 38(12):2153-2161.
pmid: 27623797 |
| [15] |
Cui YY, Ling C, Zhang YY, et al. Production of shikimic acid from Escherichia coli through chemically inducible chromosomal evolution and cofactor metabolic engineering[J]. Microb Cell Fact, 2014, 13:21.
doi: 10.1186/1475-2859-13-21 URL |
| [16] |
Cortés-Tolalpa L, Gutiérrez-Ríos RM, Martínez LM, et al. Global transcriptomic analysis of an engineered Escherichia coli strain lacking the phosphoenolpyruvate:carbohydrate phosphotransferase system during shikimic acid production in rich culture medium[J]. Microb Cell Fact, 2014, 13(1):28.
doi: 10.1186/1475-2859-13-28 pmid: 24559297 |
| [17] |
Chen K, Dou J, Tang S, et al. Deletion of the aroK gene is essential for high shikimic acid accumulation through the shikimate pathway in E. coli[J]. Bioresour Technol, 2012, 119:141-147.
doi: 10.1016/j.biortech.2012.05.100 URL |
| [18] |
Chen X, Li M, Zhou L, et al. Metabolic engineering of Escherichia coli for improving shikimate synthesis from glucose[J]. Bioresour Technol, 2014, 166:64-71.
doi: 10.1016/j.biortech.2014.05.035 URL |
| [19] |
Liu DF, Ai GM, Zheng QX, et al. Metabolic flux responses to genetic modification for shikimic acid production by Bacillus subtilis strains[J]. Microb Cell Fact, 2014, 13(1):40.
doi: 10.1186/1475-2859-13-40 URL |
| [20] | Licona-Cassani C, Lara AR, Cabrera-Valladares N, et al. Inactivation of pyruvate kinase or the phosphoenolpyruvate:sugar phosphotransferase system increases shikimic and dehydroshikimic acid yields from glucose in Bacillus subtilis[J]. J Mol Microbiol Biotechnol, 2014, 24(1):37-45. |
| [21] |
Rawat G, Tripathi P, Yadav S, et al. An interactive study of influential parameters for shikimic acid production using statistical approach, scale up and its inhibitory action on different lipases[J]. Bioresour Technol, 2013, 144:675-679.
doi: 10.1016/j.biortech.2013.06.113 URL |
| [22] |
Tripathi P, Rawat G, Yadav S, et al. Fermentative production of shikimic acid:a paradigm shift of production concept from plant route to microbial route[J]. Bioprocess Biosyst Eng, 2013, 36(11):1665-1673.
doi: 10.1007/s00449-013-0940-4 URL |
| [23] |
Tripathi P, Rawat G, Yadav S, et al. Shikimic acid, a base compound for the formulation of swine/avian flu drug:statistical optimization, fed-batch and scale up studies along with its application as an antibacterial agent[J]. Antonie Van Leeuwenhoek, 2015, 107(2):419-431.
doi: 10.1007/s10482-014-0340-z pmid: 25563634 |
| [24] |
Kogure T, Kubota T, Suda M, et al. Metabolic engineering of Corynebacterium glutamicum for shikimate overproduction by growth-arrested cell reaction[J]. Metab Eng, 2016, 38:204-216.
doi: 10.1016/j.ymben.2016.08.005 URL |
| [25] |
Zhang B, Zhou N, Liu YM, et al. Ribosome binding site libraries and pathway modules for shikimic acid synthesis with Corynebacterium glutamicum[J]. Microb Cell Fact, 2015, 14:71.
doi: 10.1186/s12934-015-0254-0 pmid: 25981633 |
| [26] |
Liu C, Zhang B, Liu YM, et al. New intracellular shikimic acid biosensor for monitoring shikimate synthesis in Corynebacterium glutamicum[J]. ACS Synth Biol, 2018, 7(2):591-601.
doi: 10.1021/acssynbio.7b00339 URL |
| [27] |
Zhang B, Jiang CY, Liu YM, et al. Engineering of a hybrid route to enhance shikimic acid production in Corynebacterium glutamicum[J]. Biotechnol Lett, 2015, 37(9):1861-1868.
doi: 10.1007/s10529-015-1852-y pmid: 25967037 |
| [28] |
Rawat G, Tripathi P, Jahan F, et al. A natural isolate producing shikimic acid:isolation, identification, and culture condition optimization[J]. Appl Biochem Biotechnol, 2013, 169(8):2290-2302.
doi: 10.1007/s12010-013-0150-1 URL |
| [29] |
Ghosh S, Banerjee UC. Generation of aroE overexpression mutant of Bacillus megaterium for the production of shikimic acid[J]. Microb Cell Fact, 2015, 14:69.
doi: 10.1186/s12934-015-0251-3 URL |
| [30] |
Wu XY, Guo XY, Zhang B, et al. Recent advances of L-ornithine biosynthesis in metabolically engineered Corynebacterium glutamicum[J]. Front Bioeng Biotechnol, 2019, 7:440.
doi: 10.3389/fbioe.2019.00440 URL |
| [31] |
Schäfer A, Tauch A, Jäger W, et al. Small mobilizable multi-purpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19:selection of defined deletions in the chromosome of Corynebacterium glutamicum[J]. Gene, 1994, 145(1):69-73.
doi: 10.1016/0378-1119(94)90324-7 pmid: 8045426 |
| [32] |
Park SH, Kim HU, Kim TY, et al. Metabolic engineering of Corynebacterium glutamicum for L-arginine production[J]. Nat Commun, 2014, 5:4618.
doi: 10.1038/ncomms5618 URL |
| [33] | Man Z, Xu M, Rao Z, et al. Systems pathway engineering of Corynebacterium crenatum for improved L-arginine production[J]. Sci Rep, 2016, 6:28629. |
| [34] |
Xu M, Li J, Shu Q, et al. Enhancement of L-arginine production by increasing ammonium uptake in an AmtR-deficient Corynebacterium crenatum mutant[J]. J Ind Microbiol Biotechnol, 2019, 46(8):1155-1166.
doi: 10.1007/s10295-019-02204-3 URL |
| [35] |
Zhang B, Gao G, Chu XH, et al. Metabolic engineering of Corynebacterium glutamicum S9114 to enhance the production of l-ornithine driven by glucose and xylose[J]. Bioresour Technol, 2019, 284:204-213.
doi: 10.1016/j.biortech.2019.03.122 URL |
| [36] |
Zhang J, Qian F, Dong F, et al. De novo engineering of Corynebacterium glutamicum for l-proline production[J]. ACS Synth Biol, 2020, 9(7):1897-1906.
doi: 10.1021/acssynbio.0c00249 pmid: 32627539 |
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| [11] | Wu Xinyang,Pei Guangsheng,Zheng Xiaomei,Cao Guoqiang,Liu Jiao,Zheng Ping,Wang Min,Sun Jibin. Phosphoenolpyruvate Carboxylase from Corynebacterium glutamicum Inhibited by Diverse Biochemicals [J]. Biotechnology Bulletin, 2013, 0(7): 172-178. |
| [12] | Shi Zengxiu, Cui Wenjing, Zhou Li, Zhou Zhemin . Cloning and Characterization of L-aspartate-α-decarboxylase from Corynebacterium glutamicum [J]. Biotechnology Bulletin, 2013, 0(4): 110-115. |
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