Metabolic Engineering for Modifying Corynebacterium glutamicum to Produce More Pyruvate

doi:10.13560/j.cnki.biotech.bull.1985.2016.08.033

Abstract

Abstract: In order to increase the accumulation of pyruvate while decrease the production of by-products during the metabolic process of Corynebacterium glutamicum,the homologous recombination was employed to knock out the key genes of pyruvate：quinone oxidoreductase （pqo）,pyruvate dehydrogenase（pdh）and L-lactate dehydrogenase（lldh）in the tributary metabolic pathways,thus the gene deletion mutation strains were constructed. Using compound medium with 4.5% glucose as carbon sources,the concentration of pyruvate with mutation strain at shake flask fermentation for 48 hours reached 14.6 g/L,while that with the wild type only was 0.45 g/L. The conversion rate of sugar to acid with the mutation strain was 33.18% and 32.5 times higher than the wild type.

Key words: pyruvate, Corynebacterium glutamicum, pyruvate：quinone oxidoreductase, pyruvate dehydrogenase, L-lactate dehydrogenase

YU Ying, XU Mei-xue ,LIU Jin-lei, FAN Rong ,FENG Hui-yong, LI Tian-ming. Metabolic Engineering for Modifying Corynebacterium glutamicum to Produce More Pyruvate[J]. Biotechnology Bulletin, 2016, 32(8): 226-232.

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