Isolation of Halophilic Heterotrophic Nitrification-aerobic Denitrification Bacterium Bacillus megatherium N07 and Its Denitrification Characteristics

doi:10.13560/j.cnki.biotech.bull.1985.2021-1111

Abstract

Abstract:

Biological nitrogen-removing technology based on heterotrophic nitrification and aerobic denitrification bacteria may effectively remove nitrogen in sewage. There are many shortcomings in the treatment of high salt wastewater,such as inhibiting microbial growth and low nitrogen removal efficiency. In order to solve the problem of difficult nitrogen removal from high salt wastewater,a strain of heterotrophic nitrification and aerobic denitrification with high salt concentration of 8%（W/V）was selected from chemical wastewater of agricultural pharmaceutical factory and named N07. The denitrification rate of the strain was measured under different salinity and dissolved oxygen conditions,and the effects of single environmental factors on the denitrification performance of the strain was investigated. The results showed that strain N07 was identified as Bacillus megatherium. It tolerated 8% salinity and reproduced vigorously. The removal rates of ammonia nitrogen and nitrite nitrogen were up to 73.61% and 83.56%,belonging to moderate halophilic bacteria. The bacterium showed high denitrification ability under aerobic conditions,with nitrate and nitrite removal rates of 11.87 mg/（L· h）and 8.26 mg/（L· h）at 12 h. The best nitrification capacity was obtained when carbon source was sucrose,C/N was 25,pH was 7,rotation speed was 160 r/min and temperature was 28℃. B. megatherium N07 can degrade nitrogen in high salinity environment,which provides reference for the practical application of heterotrophic nitrification and aerobic denitrification bacteria in the treatment of high salinity nitrogen wastewater such as coastal aquaculture industry.

Key words: heterotrophic nitrification aerobic denitrification, biological nitrogen removal, high salt wastewater, Bacillus megatherium

GAO Yu-xuan, JIN Jing-chen, XU Li-shan, GAO Ya-Juan, ZHANG Wen-tian, LI Chen-chen, ZHANG Guo-wei, JIN Yong-sheng. Isolation of Halophilic Heterotrophic Nitrification-aerobic Denitrification Bacterium Bacillus megatherium N07 and Its Denitrification Characteristics[J]. Biotechnology Bulletin, 2022, 38(7): 247-257.

Figures/Tables 12

Table 1 Comparison of nitrogen removal performance of three denitrification strains

编号No.	氨氮Ammonia nitrogen		总氮Total nitrogen
编号No.	去除率 Removal rate/%	降解速率 Degradation rate/（mg·（L·h）^-1）	去除率 Removal rate/%	降解速率 Degradation rate/（mg·（L·h）^-1）
N01	55.53	4.63	33.78	2.81
N04	50.21	4.16	24.77	2.06
N07	70.95	5.91	63.84	5.33

Fig. 1 Morphological identification of strain N07 A：Colony morphology of solid medium. B：Gram staining results. C：SEM cell morphology

Fig. 2 Phylogenetic tree of strain N07

Fig.3 Growth of strain N07 under different salinities A：Ammonia nitrogen was the only nitrogen source in the medium. B：Nitrite nitrogen was the only nitrogen source in the medium. The error line in the figure represents the standard deviation,different letters indicated significant difference,P <0.05（n=3）. The same below

Fig. 4 Nitrogen removal effect of strain N07 at different salinities A：Ammonia nitrogen was the only nitrogen source in the medium. B：Nitrite nitrogen was the only nitrogen source in the medium

Fig. 5 Degradation performance of strain N07 under different DO A：Aerobic conditions;B：Micro-anaerobic conditions

Fig. 6 Degradation of nitrite nitrogen by strain N07 under different DO A：Aerobic conditions;B：Micro-anaerobic conditions

Fig.7 Effect of carbon source on the nitrification capacity of strain N07

Fig. 8 Effects of C/N ratio on the denitrification capacity of strain N07

Fig.9 Effect of initial pH value on the denitrification capacity of strain N07

Fig.10 Effect of rotation speed on the denitrification capacity of strain N07

Fig.11 Effect of temperature on the denitrification capacity of strain N07

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