Biosafety and Nitrogen Removal Performance of a Safe and Efficient Aerobic Denitrifying Pseudomonas stutzeri DZ11

doi:10.13560/j.cnki.biotech.bull.1985.2021-1084

Abstract

Abstract:

The aerobic denitrifying bacteria separated and screened from the bottom sludge and sewage of the aquaculture farm in Minzhong town，Zhongshan city，were studied，aiming to lay the foundation for the development of the denitrification process of aquaculture wastewater. The high-performance bacterium DZ11 was selected from the samples，and its morphological observation，physiological and biochemical determination and 16S rDNA gene sequence analysis were performed to determine the species of the strain. Besides，drug susceptibility test and biological safety test were conducted to confirm the safety of strains. Then the influences of carbon source types，temperature，pH value and carbon-nitrogen ratio on the nitrogen removal efficiency were studied to improve the denitrification efficiency of strains’ performance. The identification results showed that the strain DZ11 was identified as Pseudomonas stutzeri and named Pseudomonas stutzeri DZ11. Drug susceptibility test and biological safety test demonstrated that the strain had no obvious drug resistance and had high biological safety. The result of single factor optimization is that the optimal carbon source of strain DZ11 was sodium citrate，the optimal temperature was 30.0-35.0℃，the optimal pH was 7，and the optimal C/N was in the range of 10-20. When NH₄-N，NO₃-N，and NO₂-N were the only substrates，the transformation rates were 84.39%，99.0% and 97.65%，respectively，indicating that the strain had a good denitrification effect. In sum，the strain DZ11 has high biological properties and high-efficiency denitrification performance，and has great application potential in the denitrification process of aquaculture wastewater.

Key words: aerobic denitrifying bacterium, Pseudomonas, biological removal of nitrogen, bio-safety

ZHAO Yang, SUN Hui-ming, LIN Hao-peng, LUO Ping-ting, ZHU Ya-ting, CHEN Qiong-hua, SHU Hu. Biosafety and Nitrogen Removal Performance of a Safe and Efficient Aerobic Denitrifying Pseudomonas stutzeri DZ11[J]. Biotechnology Bulletin, 2022, 38(10): 226-234.

Figures/Tables 10

References 33

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项目 Item	结果 Result	项目 Item	结果 Result
氧化酶反应Oxidase reaction	+	反硝化Denitrification	+
明胶液化Gelatin liquefaction	-	蔗糖发酵Sucrose fermentation	-
淀粉水解Starch hydrolysis	+	D-（+）-葡萄糖发酵 D-（+）-Glucose fermentation	+
吲哚生产Indole production	-	乳糖发酵Lactose fermentation	-
甲基红试验Methyl red	-	D-（+）-麦芽糖发酵 D-（+）-Maltose fermentation	+
V-P试验Vogs-Proskauer text	-	甘油Glycerol	-
Tween80	+	肌酸Creatine	₊
4℃生长Growth at 4℃	-	甘露醇Mannitol	-
41℃生长Growth at 41℃	+	D-半乳糖发酵D-Galactose fermentation	-

项目 Item	结果 Result	项目 Item	结果 Result
氧化酶反应Oxidase reaction	+	反硝化Denitrification	+
明胶液化Gelatin liquefaction	-	蔗糖发酵Sucrose fermentation	-
淀粉水解Starch hydrolysis	+	D-（+）-葡萄糖发酵 D-（+）-Glucose fermentation	+
吲哚生产Indole production	-	乳糖发酵Lactose fermentation	-
甲基红试验Methyl red	-	D-（+）-麦芽糖发酵 D-（+）-Maltose fermentation	+
V-P试验Vogs-Proskauer text	-	甘油Glycerol	-
Tween80	+	肌酸Creatine	₊
4℃生长Growth at 4℃	-	甘露醇Mannitol	-
41℃生长Growth at 41℃	+	D-半乳糖发酵D-Galactose fermentation	-

药品 Drug	抑菌环大小 Size of inhibition ring/mm	敏感等级 Sensitive level	药品 Drug	抑菌环大小 Size of inhibition ring/mm	敏感等级 Sensitive levels
诺氟沙星 Norfloxacin	44±1.73	S	环丙沙星Ciprofloxacin	41±1.73	S
氯霉素 Chloramphenicol	19.67±0.58	S	头孢他啶Ceftazidime	24.33±0.57	S
硫酸庆大霉素Gentamicin sulfate	23.67±0.58	S	左氧氟沙星Levofloxacin	32±1	S
盐酸四环素Tetracycline hydrochloride	24±1	S	链霉素 Streptomycin	19.33±0.58	S

药品 Drug	抑菌环大小 Size of inhibition ring/mm	敏感等级 Sensitive level	药品 Drug	抑菌环大小 Size of inhibition ring/mm	敏感等级 Sensitive levels
诺氟沙星 Norfloxacin	44±1.73	S	环丙沙星Ciprofloxacin	41±1.73	S
氯霉素 Chloramphenicol	19.67±0.58	S	头孢他啶Ceftazidime	24.33±0.57	S
硫酸庆大霉素Gentamicin sulfate	23.67±0.58	S	左氧氟沙星Levofloxacin	32±1	S
盐酸四环素Tetracycline hydrochloride	24±1	S	链霉素 Streptomycin	19.33±0.58	S