Biotechnology Bulletin ›› 2022, Vol. 38 ›› Issue (11): 104-111.doi: 10.13560/j.cnki.biotech.bull.1985.2022-0004
Previous Articles Next Articles
ZHANG Lin1,2(), WEI Zhen-zhen2, SONG Cheng-wei2, GUO Li-li2, GUO Qi2, HOU Xiao-gai2, WANG Hua-fang1()
Received:
2022-01-02
Online:
2022-11-26
Published:
2022-12-01
Contact:
WANG Hua-fang
E-mail:ayzhanglin@126.com;hfwang@bjfu.edu.cn
ZHANG Lin, WEI Zhen-zhen, SONG Cheng-wei, GUO Li-li, GUO Qi, HOU Xiao-gai, WANG Hua-fang. Cloning and Expression Analysis of PoFD Gene from Paeonia ostii ‘Fengdan’[J]. Biotechnology Bulletin, 2022, 38(11): 104-111.
引物名称 Primer name | 序列 Sequence(5'-3') | 扩增目的 Amplification purpose |
---|---|---|
PoFD-F | TTTACCGGACCTGAACAC | 克隆PoFD基因 Cloning of PoFD gene |
PoFD-R | ACCCAACACCCTCATACA | |
PoFD-RT-F | ATTGTAGGCGGTAGGAGCAC | PoFD基因RT-qPCR分析 RT-qPCR assay of PoFD gene |
PoFD-RT-R | TATCAAGCACGGTCGCTGT | |
Actin-F Actin-R | GGTCTATTCTTGCTTCCCTCAG GAACTCACTATCAAACCCTCCAG | RT-qPCR内参基因 RT-qPCR reference gene |
Table 1 Primer sequence
引物名称 Primer name | 序列 Sequence(5'-3') | 扩增目的 Amplification purpose |
---|---|---|
PoFD-F | TTTACCGGACCTGAACAC | 克隆PoFD基因 Cloning of PoFD gene |
PoFD-R | ACCCAACACCCTCATACA | |
PoFD-RT-F | ATTGTAGGCGGTAGGAGCAC | PoFD基因RT-qPCR分析 RT-qPCR assay of PoFD gene |
PoFD-RT-R | TATCAAGCACGGTCGCTGT | |
Actin-F Actin-R | GGTCTATTCTTGCTTCCCTCAG GAACTCACTATCAAACCCTCCAG | RT-qPCR内参基因 RT-qPCR reference gene |
Fig. 6 Expression analysis of PoFD gene in different tissues of ‘Fengdan’ Different lowercases indicate significant differences at the 0.05 level. The same below
[1] | 洪德元, 潘开玉. 芍药属牡丹组的分类历史和分类处理[J]. 植物分类学报, 1999, 37(4):351-368. |
Hong DY, Pan KY. Taxonomical history and revision of Paeonia sect. Moutan(Paeoniaceae)[J]. Acta Phytotaxon Sin, 1999, 37(4):351-368. | |
[2] |
Zhang JJ, Shu QY, Liu ZA, et al. Two EST-derived marker systems for cultivar identification in tree peony[J]. Plant Cell Rep, 2012, 31(2):299-310.
doi: 10.1007/s00299-011-1164-1 pmid: 21987120 |
[3] | 杨礼通, 张文静, 雷映雪, 等. 推迟或提前牡丹花期的调控研究进展[J]. 四川农业科技, 2021(8):80-81, 85. |
Yang LT, Zhang WJ, Lei YX, et al. Research progress on the regulation of delaying or advancing the flowering period of tree peony[J]. Sichuan Agric Sci Technol, 2021(8):80-81, 85. | |
[4] | 石丰瑞. 牡丹长日照途径关键基因PsFT以及其上、下游基因PsCO、PsSOC1的克隆与表达分析[D]. 北京: 中国农业科学院, 2013. |
Shi FR. Cloning and expression analysis of key gene PsFT of long-day photoperiod pathway and its upstream and downstream genes PsCO, PsSOCl in tree peony(Paeonia suffruticosa andr. )[D]. Beijing: Chinese Academy of Agricultural Sciences, 2013. | |
[5] | 康圣楠, 陈曦, 张雪敏, 等. 百合花期调控研究进展[J/OL]. 分子植物育种, 2021. https://kns.cnki.net/kcms/detail/46.1068.S.20210930.1435.008.html. |
Kang SN, Chen X, Zhang XM, et al. Research progress on regulation of flowering stage of Lilium brownii var[J/OL]. Mol Plant Breed, 2021. https://kns.cnki.net/kcms/detail/46.1068.S.20210930.1435.008.html. | |
[6] | 李瑞英, 任崇勇, 张婷. 影响牡丹花期的主要气象因素及预测方法[J]. 现代农业科技, 2016(13):244-245. |
Li RY, Ren CY, Zhang T. Influencing meteorological factors on peony flowering and prediction method[J]. Mod Agric Sci Technol, 2016(13):244-245. | |
[7] | 周华. 基于转录组比较的牡丹开花时间基因发掘[D]. 北京: 北京林业大学, 2015. |
Zhou H. Discovery of genes associated with flowering time in tree peonies based on transcriptome comparison[D]. Beijing: Beijing Forestry University, 2015. | |
[8] | 张彦楠, 于秀立, 吕新华, 等. 棉花bZIP转录因子FD基因的发掘和GhFD基因的组织表达分析[J]. 分子植物育种, 2016, 14(9):2250-2260. |
Zhang YN, Yu XL, Lv XH, et al. Genome-wide identification of bZIP transcription factor FD genes and expression patterns analysis of GhFD genes in cotton[J]. Mol Plant Breed, 2016, 14(9):2250-2260. | |
[9] |
Varkonyi-Gasic E, Moss SMA, Voogd C, et al. Homologs of FT, CEN and FD respond to developmental and environmental signals affecting growth and flowering in the perennial vine kiwifruit[J]. New Phytol, 2013, 198(3):732-746.
doi: 10.1111/nph.12162 pmid: 23577598 |
[10] |
Ryu JY, Lee HJ, Seo PJ, et al. The Arabidopsis floral repressor BFT delays flowering by competing with FT for FD binding under high salinity[J]. Mol Plant, 2014, 7(2):377-387.
doi: 10.1093/mp/sst114 URL |
[11] |
Kawamoto N, Sasabe M, Endo M, et al. Calcium-dependent protein kinases responsible for the phosphorylation of a bZIP transcription factor FD crucial for the florigen complex formation[J]. Sci Rep, 2015, 5:8341.
doi: 10.1038/srep08341 pmid: 25661797 |
[12] | 蔡芳芳. 二球悬铃木FT和FD同源基因的功能分析及选择性剪切初探[D]. 武汉: 华中农业大学, 2019. |
Cai FF. Functional and alternative splicing analysis of FT and FD homologous genes in Platanus acerifolia[D]. Wuhan: Huazhong Agricultural University, 2019. | |
[13] | 曹青霞. 茶树CsFD基因克隆及其与CsFT基因在成花转变中的功能研究[D]. 西安: 陕西师范大学, 2018. |
Cao QX. The CsFD gene cloning of tea plant and the study of its function with CsFT gene in flowering transition[D]. Xi’an: Shaanxi Normal University, 2018. | |
[14] |
袁敏, 葛伟娜, 王莉, 等. 拟南芥蛋白激酶SnRK1. 1对转录因子FD的磷酸化分析[J]. 华北农学报, 2017, 32(4):37-41.
doi: 10.7668/hbnxb.2017.04.006 |
Yuan M, Ge WN, Wang L, et al. Protein kinase SnRK1. 1 phosphorylates transcriptional factor FD in Arabidopsis thaliana[J]. Acta Agric Boreali Sin, 2017, 32(4):37-41. | |
[15] |
Taoka KI, Ohki I, Tsuji H, et al. 14-3-3 proteins act as intracellular receptors for rice Hd3a florigen[J]. Nature, 2011, 476(7360):332-335.
doi: 10.1038/nature10272 URL |
[16] |
Li ZC, Ou Y, Zhang ZC, et al. Brassinosteroid signaling recruits histone 3 lysine-27 demethylation activity to FLOWERING LOCUS C chromatin to inhibit the floral transition in Arabidopsis[J]. Mol Plant, 2018, 11(9):1135-1146.
doi: 10.1016/j.molp.2018.06.007 URL |
[17] | 侯倩倩. MdFD基因的功能验证及苹果早花相关基因的表达分析[D]. 北京: 中国农业科学院, 2018. |
Hou QQ. Functional verification of MdFD gene and expression analysis of early flower related genes in apple[D]. Beijing: Chinese Academy of Agricultural Sciences, 2018. | |
[18] |
Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method[J]. Methods, 2001, 25(4):402-408.
doi: 10.1006/meth.2001.1262 pmid: 11846609 |
[19] |
Blümel M, Dally N, Jung C. Flowering time regulation in crops—what did we learn from Arabidopsis?[J]. Curr Opin Biotechnol, 2015, 32:121-129.
doi: 10.1016/j.copbio.2014.11.023 URL |
[20] |
Vollrath P, Chawla HS, Schiessl SV, et al. A novel deletion in FLOWERING LOCUS T modulates flowering time in winter oilseed rape[J]. Theor Appl Genet, 2021, 134(4):1217-1231.
doi: 10.1007/s00122-021-03768-4 pmid: 33471161 |
[21] |
Alves MS, Dadalto SP, Gonçalves AB, et al. Plant bZIP transcription factors responsive to pathogens:a review[J]. Int J Mol Sci, 2013, 14(4):7815-7828.
doi: 10.3390/ijms14047815 URL |
[22] |
Lee BJ, Park CJ, Kim SK, et al. In vivo binding of hot pepper bZIP transcription factor CabZIP1 to the G-box region of pathogenesis-related protein 1 promoter[J]. Biochem Biophys Res Commun, 2006, 344(1):55-62.
doi: 10.1016/j.bbrc.2006.03.153 URL |
[23] | 段亚宾. 牡丹开花相关基因的克隆和表达分析[D]. 洛阳: 河南科技大学, 2015. |
Duan YB. Cloning and expression of flowering genes in tree peony[D]. Luoyang: Henan University of Science and Technology, 2015. | |
[24] | 罗轩, 丛汉卿, 李丽, 等. 蜻蜓凤梨FLD同源基因的克隆及表达分析[J]. 分子植物育种, 2013, 11(3):371-378. |
Luo X, Cong HQ, Li L, et al. Cloning and expression analysis of FLD homologous gene from Aechmea fasciata[J]. Mol Plant Breed, 2013, 11(3):371-378. | |
[25] | 罗玉秀, 张生萍, 许唱唱, 等. 特早熟春性甘蓝型油菜sBnFLD基因的克隆及表达[J]. 西北农林科技大学学报:自然科学版, 2016, 44(11):90-96. |
Luo YX, Zhang SP, Xu CC, et al. Cloning and expression of sBnFLD gene from spring rapeseed(Brassica napus L.)[J]. J Northwest A&F Univ Nat Sci Ed, 2016, 44(11):90-96. | |
[26] |
Tsuji H, Nakamura H, Taoka KI, et al. Functional diversification of FD transcription factors in rice, components of florigen activation complexes[J]. Plant Cell Physiol, 2013, 54(3):385-397.
doi: 10.1093/pcp/pct005 pmid: 23324168 |
[27] |
Wigge PA, Kim MC, Jaeger KE, et al. Integration of spatial and temporal information during floral induction in Arabidopsis[J]. Science, 2005, 309(5737):1056-1059.
doi: 10.1126/science.1114358 URL |
[28] |
Li CX, Dubcovsky J. Wheat FT protein regulates VRN1 transcription through interactions with FDL2[J]. Plant J, 2008, 55(4):543-554.
doi: 10.1111/j.1365-313X.2008.03526.x URL |
[29] |
Andrés F, Coupland G. The genetic basis of flowering responses to seasonal cues[J]. Nat Rev Genet, 2012, 13(9):627-639.
doi: 10.1038/nrg3291 pmid: 22898651 |
[30] |
Dutta S, Deb A, Biswas P, et al. Identification and functional characterization of two bamboo FD gene homologs having contrasting effects on shoot growth and flowering[J]. Sci Rep, 2021, 11(1):7849.
doi: 10.1038/s41598-021-87491-6 URL |
[31] | Jang S, Choi SC, Li HY, et al. Functional characterization of Phalaenopsis aphrodite flowering genes PaFT1 and PaFD[J]. PLoS One, 2015, 10(8):e0134987. |
[32] | 张玲. 枇杷花期调控的分子生物学研究[D]. 广州: 华南农业大学, 2016. |
Zhang L. Studies on molecular mechanism of loquat flowering time regulation[D]. Guangzhou: South China Agricultural University, 2016. |
[1] | LYU Qiu-yu, SUN Pei-yuan, RAN Bin, WANG Jia-rui, CHEN Qing-fu, LI Hong-you. Cloning, Subcellular Localization and Expression Analysis of the Transcription Factor Gene FtbHLH3 in Fagopyrum tataricum [J]. Biotechnology Bulletin, 2023, 39(8): 194-203. |
[2] | WANG Jia-rui, SUN Pei-yuan, KE Jin, RAN Bin, LI Hong-you. Cloning and Expression Analyses of C-glycosyltransferase Gene FtUGT143 in Fagopyrum tataricum [J]. Biotechnology Bulletin, 2023, 39(8): 204-212. |
[3] | SUN Ming-hui, WU Qiong, LIU Dan-dan, JIAO Xiao-yu, WANG Wen-jie. Cloning and Expression Analysis of CsTMFs Gene in Tea Plant [J]. Biotechnology Bulletin, 2023, 39(7): 151-159. |
[4] | ZHAO Xue-ting, GAO Li-yan, WANG Jun-gang, SHEN Qing-qing, ZHANG Shu-zhen, LI Fu-sheng. Cloning and Expression of AP2/ERF Transcription Factor Gene ShERF3 in Sugarcane and Subcellular Localization of Its Encoded Protein [J]. Biotechnology Bulletin, 2023, 39(6): 208-216. |
[5] | JIANG Qing-chun, DU Jie, WANG Jia-cheng, YU Zhi-he, WANG Yun, LIU Zhong-yu. Expression and Function Analysis of Transcription Factor PcMYB2 from Polygonum cuspidatum [J]. Biotechnology Bulletin, 2023, 39(5): 217-223. |
[6] | YAO Zi-ting, CAO Xue-ying, XIAO Xue, LI Rui-fang, WEI Xiao-mei, ZOU Cheng-wu, ZHU Gui-ning. Screening of Reference Genes for RT-qPCR in Neoscytalidium dimidiatum [J]. Biotechnology Bulletin, 2023, 39(5): 92-102. |
[7] | WANG Yi-qing, WANG Tao, WEI Chao-ling, DAI Hao-min, CAO Shi-xian, SUN Wei-jiang, ZENG Wen. Identification and Interaction Analysis of SMAS Gene Family in Tea Plant(Camellia sinensis) [J]. Biotechnology Bulletin, 2023, 39(4): 246-258. |
[8] | LIU Si-jia, WANG Hao-nan, FU Yu-chen, YAN Wen-xin, HU Zeng-hui, LENG Ping-sheng. Cloning and Functional Analysis of LiCMK Gene in Lilium ‘Siberia’ [J]. Biotechnology Bulletin, 2023, 39(3): 196-205. |
[9] | PING Huai-lei, GUO Xue, YU Xiao, SONG Jing, DU Chun, WANG Juan, ZHANG Huai-bi. Cloning and Expression of PdANS in Paeonia delavayi and Correlation with Anthocyanin Content [J]. Biotechnology Bulletin, 2023, 39(3): 206-217. |
[10] | WANG Tao, QI Si-yu, WEI Chao-ling, WANG Yi-qing, DAI Hao-min, ZHOU Zhe, CAO Shi-xian, ZENG Wen, SUN Wei-jiang. Expression Analysis and Interaction Protein Validation of CsPPR and CsCPN60-like in Albino Tea Plant(Camellia sinensis) [J]. Biotechnology Bulletin, 2023, 39(3): 218-231. |
[11] | PANG Qiang-qiang, SUN Xiao-dong, ZHOU Man, CAI Xing-lai, ZHANG Wen, WANG Ya-qiang. Cloning of BrHsfA3 in Chinese Flowering Cabbage and Its Responses to Heat Stress [J]. Biotechnology Bulletin, 2023, 39(2): 107-115. |
[12] | MIAO Shu-nan, GAO Yu, LI Xin-ru, CAI Gui-ping, ZHANG Fei, XUE Jin-ai, JI Chun-li, LI Run-zhi. Functional Analysis of Soybean GmPDAT1 Genes in the Oil Biosynthesis and Response to Abiotic Stresses [J]. Biotechnology Bulletin, 2023, 39(2): 96-106. |
[13] | GE Wen-dong, WANG Teng-hui, MA Tian-yi, FAN Zhen-yu, WANG Yu-shu. Genome-wide Identification of the PRX Gene Family in Cabbage(Brassica oleracea L. var. capitata)and Expression Analysis Under Abiotic Stress [J]. Biotechnology Bulletin, 2023, 39(11): 252-260. |
[14] | YANG Xu-yan, ZHAO Shuang, MA Tian-yi, BAI Yu, WANG Yu-shu. Cloning of Three Cabbage WRKY Genes and Their Expressions in Response to Abiotic Stress [J]. Biotechnology Bulletin, 2023, 39(11): 261-269. |
[15] | CHEN Chu-yi, YANG Xiao-mei, CHEN Sheng-yan, CHEN Bin, YUE Li-ran. Expression Analysis of the ZF-HD Gene Family in Chrysanthemum nankingense Under Drought and ABA Treatment [J]. Biotechnology Bulletin, 2023, 39(11): 270-282. |
Viewed | ||||||
Full text |
|
|||||
Abstract |
|
|||||