Expression Analysis and Interaction Protein Validation of CsPPR and CsCPN60-like in Albino Tea Plant（Camellia sinensis）

doi:10.13560/j.cnki.biotech.bull.1985.2022-0802

Abstract

Abstract:

Our group screened out two genes（CSS0013384 and CSS0036305）related to the albino leaf color of Camellia sinensis Baijiguan through the transcriptome, in order to explore the expression patterns of CSS0013384 and CSS0036305 in albino tea plants interacting proteins. Using cultivar Baijiguan as material, the full-length cDNA sequences of CSS0013384 and CSS0036305 were cloned. Bioinformatics, yeast one-hybrid and yeast two-hybrid were used to analyze the protein physicochemical properties, the phylogenetic tree, the chromosome location, the gene structure, the protein structure, the protein regulation and interaction network, and gene expression pattern. Bioinformatics analysis showed that CSS0013384 and CSS0036305 belonged to the pentatricopeptide repeat protein（PPR）and chaperone（CPN60-like）families, respectively. The lengths of their protein coding sequence（CDS）was 1 893 bp and 1 752 bp, the number of encoded amino acids was 631 and 575, the protein mass was 71.87 kD and 60.79 kD, the isoelectric points were 8.93 and 6.21, the subcellular localization prediction results indicated that CSS0013384 was localized in chloroplast, and CSS0036305 was localized in mitochondria. The RT-qPCR results of the second leaf of Baijiguan under shading and restored light treatment and tea plant varieties with different leaf colors revealed that CSS0013384 and CSS0036305 were highly expressed in albino leaves. CSS0002807 belonged to the PIF transcription factor family, and the yeast one-hybrid results indicated that CSS0002807 bound to the CSS0013384 promoter. CSS0013384 and CSS0036305 may be involved in the development of chloroplast and mitochondria in albino tea leaves, and play an important role in the process of leaf albino. The results may provide a reference for further research on the mechanism of tea leaf albino.

Key words: Camellia sinensis, albino, pentatricopeptide repeat protein, chaperone, expression analysis, interacting proteins

WANG Tao, QI Si-yu, WEI Chao-ling, WANG Yi-qing, DAI Hao-min, ZHOU Zhe, CAO Shi-xian, ZENG Wen, SUN Wei-jiang. Expression Analysis and Interaction Protein Validation of CsPPR and CsCPN60-like in Albino Tea Plant（Camellia sinensis）[J]. Biotechnology Bulletin, 2023, 39(3): 218-231.

Figures/Tables 11

References 48

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基因名称 Gene name	上游引物 Sense primer（5'-3'）	下游引物 Anti-sense primer（5'-3'）	备注 Note
CSS0013384	ATGGCCATCCCCAGTTCTCCAGACTGGTCTGTG	TTACATGGTGCCAAAGCTCTGTTGCAACCATTTTGC	基因克隆引物
CSS0036305	ATGTACAGAGTTGGTGCAGCCTTCGCTTCATCGAT	TCAGTAACCCATATCATCCAAATTTGGCATTCGAT	基因克隆引物
qCSS0013384	TGGGGAAAACAGAAGGGCTAA	CAACACCACCACCTGGAACAA	荧光定量引物
qCSS0036305	CCAAAATGAAGGAAAGAGGGTG	TCAAAGCGGAGCGATAGAGC	荧光定量引物
β-Actin	GCCATCTTTGATTGGAATGG	GGTGCCACAACCTTGATCTT	RNA验证
pAbAi- CSS0013384pro	AAATGATGAATTGAAAAGCTTAAGAAATTTTTTGTTGAG	ATACAGAGCACATGCCTCGAGAATTTTGCCTTGCATCAT	酵母单杂交
AD-CSS0002807	GCCATGGAGGCCAGTGAATTCATGAAAGGAATCATGAA	CTCGATGGATCTCACTTACTTGAGCTAGAA	酵母单杂交
BK-CSS0003881	ATGGCCATGGAGGCCGAATTCATGGCATCAACTTTGGCT	CCGCTGCAGGTCGACGGATCTTAGTAACCGTATCCTGA	酵母双杂交
BK-CSS0041773	ATGGCCATGGAGGCCGAATTCATGGCATCAACTTTGGCT	CCGCTGCAGGTCGACGGATCTTAGTAACCGTATCCTGA	酵母双杂交
BK-CSS0043610	ATGGCCATGGAGGCCGAATTCATGTACCGCTTCGCAG	CCGCTGCAGGTCGACGGATCTCAAAAATCCATACCACC	酵母双杂交
AD-CSS0036305	GCCATGGAGGCCAGTGAATTCATGTACAGAGTTGGTGC	CAGCTCGAGCTCGATGGATCTCAGTAACCCATATCATCC	酵母双杂交
BK-CSS0036305	ATGGCCATGGAGGCCGAATTCATGTACAGAGTTGGTGCA	CCGCTGCAGGTCGACGGATCTCAGTAACCCATATCATCC	酵母双杂交
AD-CSS0003881	GCCATGGAGGCCAGTGAATTCATGGCATCAACTTTGGCT	CAGCTCGAGCTCGATGGATCTTAGTAACCGTATCCTGA	酵母双杂交
AD-CSS0041773	GCCATGGAGGCCAGTGAATTCATGGCATCAACTTTGGCT	CAGCTCGAGCTCGATGGATCTTAGTAACCGTATCCTGA	酵母双杂交
AD-CSS0043610	GCCATGGAGGCCAGTGAATTCATGTACCGCTTCGCAG	CAGCTCGAGCTCGATGGATCTCAAAAATCCATACCAC	酵母双杂交

基因名称 Gene name	上游引物 Sense primer（5'-3'）	下游引物 Anti-sense primer（5'-3'）	备注 Note
CSS0013384	ATGGCCATCCCCAGTTCTCCAGACTGGTCTGTG	TTACATGGTGCCAAAGCTCTGTTGCAACCATTTTGC	基因克隆引物
CSS0036305	ATGTACAGAGTTGGTGCAGCCTTCGCTTCATCGAT	TCAGTAACCCATATCATCCAAATTTGGCATTCGAT	基因克隆引物
qCSS0013384	TGGGGAAAACAGAAGGGCTAA	CAACACCACCACCTGGAACAA	荧光定量引物
qCSS0036305	CCAAAATGAAGGAAAGAGGGTG	TCAAAGCGGAGCGATAGAGC	荧光定量引物
β-Actin	GCCATCTTTGATTGGAATGG	GGTGCCACAACCTTGATCTT	RNA验证
pAbAi- CSS0013384pro	AAATGATGAATTGAAAAGCTTAAGAAATTTTTTGTTGAG	ATACAGAGCACATGCCTCGAGAATTTTGCCTTGCATCAT	酵母单杂交
AD-CSS0002807	GCCATGGAGGCCAGTGAATTCATGAAAGGAATCATGAA	CTCGATGGATCTCACTTACTTGAGCTAGAA	酵母单杂交
BK-CSS0003881	ATGGCCATGGAGGCCGAATTCATGGCATCAACTTTGGCT	CCGCTGCAGGTCGACGGATCTTAGTAACCGTATCCTGA	酵母双杂交
BK-CSS0041773	ATGGCCATGGAGGCCGAATTCATGGCATCAACTTTGGCT	CCGCTGCAGGTCGACGGATCTTAGTAACCGTATCCTGA	酵母双杂交
BK-CSS0043610	ATGGCCATGGAGGCCGAATTCATGTACCGCTTCGCAG	CCGCTGCAGGTCGACGGATCTCAAAAATCCATACCACC	酵母双杂交
AD-CSS0036305	GCCATGGAGGCCAGTGAATTCATGTACAGAGTTGGTGC	CAGCTCGAGCTCGATGGATCTCAGTAACCCATATCATCC	酵母双杂交
BK-CSS0036305	ATGGCCATGGAGGCCGAATTCATGTACAGAGTTGGTGCA	CCGCTGCAGGTCGACGGATCTCAGTAACCCATATCATCC	酵母双杂交
AD-CSS0003881	GCCATGGAGGCCAGTGAATTCATGGCATCAACTTTGGCT	CAGCTCGAGCTCGATGGATCTTAGTAACCGTATCCTGA	酵母双杂交
AD-CSS0041773	GCCATGGAGGCCAGTGAATTCATGGCATCAACTTTGGCT	CAGCTCGAGCTCGATGGATCTTAGTAACCGTATCCTGA	酵母双杂交
AD-CSS0043610	GCCATGGAGGCCAGTGAATTCATGTACCGCTTCGCAG	CAGCTCGAGCTCGATGGATCTCAAAAATCCATACCAC	酵母双杂交

基因 Gene	分子量 Mw/kD	等电点 pI	不稳定系数 Instability index	脂肪族氨基酸指数Aliphatic index	平均疏水性 GRAVY	信号肽 Signal peptide	跨膜结构 Transmembrane domain	亚细胞定位预测 Subcellular localization
CSS0013384	71.87	8.93	40.73	85.90	-0.167	无	无	叶绿体
CSS0036305	60.79	6.21	21.85	103.99	0.002	无	无	线粒体

基因 Gene	分子量 Mw/kD	等电点 pI	不稳定系数 Instability index	脂肪族氨基酸指数Aliphatic index	平均疏水性 GRAVY	信号肽 Signal peptide	跨膜结构 Transmembrane domain	亚细胞定位预测 Subcellular localization
CSS0013384	71.87	8.93	40.73	85.90	-0.167	无	无	叶绿体
CSS0036305	60.79	6.21	21.85	103.99	0.002	无	无	线粒体

类型Type	基因名称Gene name		注释Function
类型Type	CSS0013384	CSS0036305	注释Function
光响应 Light response	A-box（2）、ATCT-motif（1）、Box 4（6）、Box Ⅱ（1）、GA-motif（1）、GATA-motif（1）、G-box（5）、I-box（1）	Box 4（1）、Box Ⅱ（1）、GATA-motif（3）、G-box（2）、GT1-motif（2）、TCCC-motif（1）、TCT-motif（2）、	参与光反应的顺式作用元件cis-acting element involved in light responsiveness
转录因子结合位点 Transcription factor binding site	MRE（1）、MBS（1）、MBSI（1）、Myb-binding site（1）	HD-Zip3（1）、MRE（1）、MBS（2）	转录因子结合位点 Transcription factor binding site
激素响应 Hormone response	AAGAA-motif（1）、ABRE（2）、ABRE2（1）	—	参与脱落酸反应的顺式作用元件cis-acting element involved in the abscisic acid responsiveness
	ERE（3）	—	参与乙烯反应的顺式作用元件 cis-acting element involved in ethylene responsiveness
	TCA-element（1）	TCA-element（2）	与水杨酸反应有关的顺式作用元件 cis-acting element involved in salicylic acid responsiveness
	—	as-1（3）、CGTCA-motif（3）、TGACG-motif（3）	参与MeJA反应的顺式作用元件 cis-acting element involved in MeJA responsiveness
组织特异性 Tissue specificity	CAT-box（2）、CCGTCC motif（4）、GCN4 motif（1）	—	组织特异性元件 Tissue specific element
胁迫响应 Stress response	ARE（1）、STRE（1）、WUN-motif（4）	ACE（1）、ARE（4）、STRE（2）、WRE3（2）、WUN-motif（2）	胁迫响应元件Stress response element