Semi-lethal Low Temperature and Taxane Content of Taxus Under Low Temperature Stress

doi:10.13560/j.cnki.biotech.bull.1985.2022-0762

Abstract

Abstract:

To determine the electrical impedance parameters suitable for the determination of semi-lethal low temperature of Taxus and explore the effects of low temperature on the taxane biosynthesis in Taxus, the electrical impedance parameters and taxane content changes of Taxus under low temperature stress were examined. It will lay a theoretical foundation for expanding the introduction range of Taxus and using low temperature conditions to increase the contents of taxane content in Taxus. Using T. yunnanensis, T. chinensis var. mairei （Changzhi, Shanxi）, T. chinensis, T. cuspidata and T. × media as materials, the semi-lethal low temperature of these trees were determined by conductivity method and electrical impedance method respectively. Moreover, the taxane contents and abscisic acid contents in the leaves of Taxus under low-temperature stress were determined by LC-MS method. The results showed that T. chinensis, T. chinensis var. mairei（Changzhi, Shanxi）, T. cuspidata and T. × media all tolerated low temperature of -14.668- -9.106℃. The T. yunnanensis tolerated -8.802- -3.521℃, which was higher than the ons of other four Taxus species. The semi-lethal low temperature of one-year-old branches of five Taxus measured by electrical impedance τ_m parameter was significantly correlated with that measured by conductivity method, and the correlation coefficient was 0.912. The taxol content in the leaves of one-year-old Taxus was higher than that in the current-year under low temperature stress. The taxol content in the leaves of one-year-old T. yunnanensis and T. cuspidata significantly increased with the decrease of treatment temperature, while the baccatin III content and 10-deacetyltaxol content in the leaves of one-year-old T. chinensis significantly increased. Moreover, ABA content in the leaves of Taxus increased with the decrease of temperature, and ABA content in the current-year leaves was higher than that in the one-year-old leaves. Compared with T. yunnanensis, T. chinensis, T. chinensis var. mairei（Changzhi, Shanxi）, T. cuspidata and T. × media tolerated lower temperature. Low temperature is beneficial to the synthesis of taxol in the leaves of one-year-old T. yunnanensis and T. cuspidata, and the synthesis of baccatin III and 10-deacetyltaxol in the leaves of current-year T. chinensis.

Key words: Taxus, low temperature stress, electrical impedance, semi-lethal low temperature, taxane

JIANG Lu-yuan, FENG Mei-jing, DU Yu-qing, DI Bao, CHEN Duan-fen, QIU De-you, YANG Yan-fang. Semi-lethal Low Temperature and Taxane Content of Taxus Under Low Temperature Stress[J]. Biotechnology Bulletin, 2023, 39(3): 232-242.

Figures/Tables 10

References 35

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编号 Code number	种名 Species	拉丁名 Latin name	来源 Resource
TY	云南红豆杉	T. yunnanensis	云南腾冲
TCV	南方红豆杉	T. chinensis var. mairei	山西长治
TC	中国红豆杉	T. chinensis（Pilger）Rehd.	甘肃陇南
TM	曼地亚红豆杉	T. × media cv. Hicksii	山东济南
TCU	东北红豆杉	T. cuspidata Sieb. et Zucc	黑龙江牡丹江

编号 Code number	种名 Species	拉丁名 Latin name	来源 Resource
TY	云南红豆杉	T. yunnanensis	云南腾冲
TCV	南方红豆杉	T. chinensis var. mairei	山西长治
TC	中国红豆杉	T. chinensis（Pilger）Rehd.	甘肃陇南
TM	曼地亚红豆杉	T. × media cv. Hicksii	山东济南
TCU	东北红豆杉	T. cuspidata Sieb. et Zucc	黑龙江牡丹江

叶/枝Leaves/branches	种Species	回归方程Logistic equation	决定系数R²	半致死温度 Semi-lethal low temperature LT₅₀/℃
当年生叶 Current-year leaves	云南红豆杉T. yunnanensis	y=75.01/（1+e^-（-3.00-^x^））+24.12	0.989	-3.521
	中国红豆杉T. chinensis（Pilger）Rehd	y=63.37/（1+e^-（-12.00-^x^））+34.25	0.993	-11.600
	南方红豆杉T. chinensis var. mairei	y=72.81/（1+e^-（-12.00-^x^））+21.70	0.996	-11.426
	东北红豆杉 T. cuspidata Sieb. et Zucc	y=63.35/（1+e^-（-12.00-^x^））+36.18	0.992	-9.611
一年生叶 One-year-old leaves	云南红豆杉T. yunnanensis	y=72.15/（1+e^-（-7.00-^x^））+26.41	0.998	-8.802
	中国红豆杉T. chinensis（Pilger）Rehd	y=82.18/（1+e^-（-12.00-^x^））+16.05	0.997	-9.673
	南方红豆杉T. chinensis var. mairei	y=76.03/（1+e^-（-12.50-^x^））+22.12	0.999	-11.225
	东北红豆杉 T. cuspidata Sieb. et Zucc	y=72.79/（1+e^-（-12.00-^x^））+23.22	0.997	-10.937
	曼地亚红豆杉T. × media cv. Hicksii	y=71.34/（1+e^-（-12.00-^x^））+46.00	0.963	-9.106
当年生枝 Current-year branches	云南红豆杉T. yunnanensis	y=30.41/（1+e^-（-3.00-^x^））+69.59	0.981	-6.806
	中国红豆杉T. chinensis（Pilger）Rehd	y=18.59/（1+e^-（-12.00-^x^））+77.98	0.998	-11.103
	南方红豆杉T. chinensis var. mairei	y=16.08/（1+e^-（-10.00-^x^））+78.74	0.994	-11.385
	东北红豆杉 T. cuspidata Sieb. et Zucc	y=16.32/（1+e^-（-12.00-^x^））+82.36	0.940	-12.731
一年生枝 One-year-old branches	云南红豆杉T. yunnanensis	y=29.94/（1+e^-（-2.00-^x^））+68.86	0.915	-7.479
	中国红豆杉T. chinensis（Pilger）Rehd	y=25.11/（1+e^-（-12.00-^x^））+74.89	0.967	-9.626
	南方红豆杉T. chinensis var. mairei	y=17.52/（1+e^-（-11.00-^x^））+82.48	0.987	-12.536
	东北红豆杉 T. cuspidata Sieb. et Zucc	y=19.94/（1+e^-（-12.00-^x^））+80.06	0.934	-14.191
	曼地亚红豆杉T. × media cv. Hicksii	y=24.47/（1+e^-（-15.00-^x^））+73.12	0.917	-14.572

叶/枝Leaves/branches	种Species	回归方程Logistic equation	决定系数R²	半致死温度 Semi-lethal low temperature LT₅₀/℃
当年生叶 Current-year leaves	云南红豆杉T. yunnanensis	y=75.01/（1+e^-（-3.00-^x^））+24.12	0.989	-3.521
	中国红豆杉T. chinensis（Pilger）Rehd	y=63.37/（1+e^-（-12.00-^x^））+34.25	0.993	-11.600
	南方红豆杉T. chinensis var. mairei	y=72.81/（1+e^-（-12.00-^x^））+21.70	0.996	-11.426
	东北红豆杉 T. cuspidata Sieb. et Zucc	y=63.35/（1+e^-（-12.00-^x^））+36.18	0.992	-9.611
一年生叶 One-year-old leaves	云南红豆杉T. yunnanensis	y=72.15/（1+e^-（-7.00-^x^））+26.41	0.998	-8.802
	中国红豆杉T. chinensis（Pilger）Rehd	y=82.18/（1+e^-（-12.00-^x^））+16.05	0.997	-9.673
	南方红豆杉T. chinensis var. mairei	y=76.03/（1+e^-（-12.50-^x^））+22.12	0.999	-11.225
	东北红豆杉 T. cuspidata Sieb. et Zucc	y=72.79/（1+e^-（-12.00-^x^））+23.22	0.997	-10.937
	曼地亚红豆杉T. × media cv. Hicksii	y=71.34/（1+e^-（-12.00-^x^））+46.00	0.963	-9.106
当年生枝 Current-year branches	云南红豆杉T. yunnanensis	y=30.41/（1+e^-（-3.00-^x^））+69.59	0.981	-6.806
	中国红豆杉T. chinensis（Pilger）Rehd	y=18.59/（1+e^-（-12.00-^x^））+77.98	0.998	-11.103
	南方红豆杉T. chinensis var. mairei	y=16.08/（1+e^-（-10.00-^x^））+78.74	0.994	-11.385
	东北红豆杉 T. cuspidata Sieb. et Zucc	y=16.32/（1+e^-（-12.00-^x^））+82.36	0.940	-12.731
一年生枝 One-year-old branches	云南红豆杉T. yunnanensis	y=29.94/（1+e^-（-2.00-^x^））+68.86	0.915	-7.479
	中国红豆杉T. chinensis（Pilger）Rehd	y=25.11/（1+e^-（-12.00-^x^））+74.89	0.967	-9.626
	南方红豆杉T. chinensis var. mairei	y=17.52/（1+e^-（-11.00-^x^））+82.48	0.987	-12.536
	东北红豆杉 T. cuspidata Sieb. et Zucc	y=19.94/（1+e^-（-12.00-^x^））+80.06	0.934	-14.191
	曼地亚红豆杉T. × media cv. Hicksii	y=24.47/（1+e^-（-15.00-^x^））+73.12	0.917	-14.572

种 Species	EL测得的半致死温度 Semi-lethal temperature by EL/℃	EIS-r_e得到的半致死温度 Semi-lethal temperature by EIS-r_e/℃	EIS-τ_m得到的半致死温度 Semi-lethal temperature by EIS-τ_m/℃	EIS-β得到的半致死温度 Semi-lethal temperature by EIS-β/℃
云南红豆杉T. yunnanensis	-7.479	-7.538	-7.287	-8.346
中国红豆杉T. chinensis（Pilger）Rehd	-9.626	-9.923	-9.305	-10.493
南方红豆杉T. chinensis var. mairei	-12.536	-12.073	-12.312	-11.603
东北红豆杉T. cuspidata Sieb. et Zucc	-14.191	-12.043	-11.061	-17.285
曼地亚红豆杉T. × media cv. Hicksii	-14.572	-10.290	-12.087	-11.422
相关系数Correlation coefficent	1	0.801	0.912*	0.734