Biotechnology Bulletin ›› 2023, Vol. 39 ›› Issue (6): 208-216.doi: 10.13560/j.cnki.biotech.bull.1985.2022-1400

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Cloning and Expression of AP2/ERF Transcription Factor Gene ShERF3 in Sugarcane and Subcellular Localization of Its Encoded Protein

ZHAO Xue-ting1(), GAO Li-yan2, WANG Jun-gang2, SHEN Qing-qing1, ZHANG Shu-zhen2(), LI Fu-sheng1()   

  1. 1. College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201
    2. Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101
  • Received:2022-11-14 Online:2023-06-26 Published:2023-07-07
  • Contact: ZHANG Shu-zhen, LI Fu-sheng E-mail:1421389929@qq.com;zhangsz2007@163.com;lfs810@sina.com

Abstract:

AP2/ERF transcription factors play an important role on regulating plant growth and development and involving in biotic and abiotic stresses resistances in plants. Cloning and gene function analysis of ShERF3 may provide gene resource for sugarcane resistance genetic improvement. The ShERF3 gene was cloned from‘ROC22’sugarcane plants based on the transcriptome data. Then the structure, subcellular location and gene expression pattern of ShERF3 were analyzed with real time PCR, bioinformation and rice protoplast subcellular location methods. The results showed that 1 142 bp cDNA sequence of ShERF3 was obtained. It contained a 1 053 bp integral opening reading frame encoding 350 amino acids. The predicted encoding protein of ShERF3 containing a conserved AP2 domain belonged to the ERF subgroup of AP2/ERF family. The homology analysis indicated that ShERF3 was highly homologous with ERF3 from Sorghum bicolor and Panicum virgatum and ERF118 from Panicum hallii and Setaria italica. It predicted that ShERF3 is a unstable hydrophobic protein. The subcellular location analysis showed that ShERF3 located in the nulear of rice protoplast cells. ShERF3 mainly expressed in sugarcane mature internodes and relatively lower expressed in the leaves and roots. In addition, the expression of ShERF3 was firstly down-regulated and then up-regulated under PEG treatment. And the expression of ShERF3 decreased with the extension of stress time under NaCl treatment. These results indicates that ShERF3 actively responds to draught and salt stresses. It may play a key role on regulating sugarcane stem development, draught and salt stresses responses.

Key words: sugarcane, ShERF3, abiotic stress, expression analysis