Biotechnology Bulletin ›› 2023, Vol. 39 ›› Issue (6): 199-207.doi: 10.13560/j.cnki.biotech.bull.1985.2022-0758

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Physiological Characteristics and Transcriptome Analysis of Sorghum bicolor × S. Sudanense Seedlings Under Salt-alkali Stress

KONG De-zhen1(), DUAN Zhen-yu1, WANG Gang1, ZHANG Xin1(), XI Lin-qiao2   

  1. 1. Institute of Crop Research, Xinjiang Academy of Agri-Reclamation Sciences, Key Lab of Xinjiang Production and Construction Corps for Cereal Quality Research and Genetic Improvement, Shihezi 832000
    2. Tarim University, Husbandry Science and Technology Key Laboratory of Production and Construction Corps of Tarim in Xinjiang, Alar 843300
  • Received:2022-06-22 Online:2023-06-26 Published:2023-07-07
  • Contact: ZHANG Xin E-mail:kongdezhen1746@163.com;zhangx0322@126.com

Abstract:

Sorghum bicolor × S. sudanense has the characteristics of drought resistance and salt-alkali tolerance, and has gradually become an important feed crop in animal husbandry. It is of great significance to clarify the molecular regulation mechanism of salt-tolerant and alkali-tolerant grass for molecular-assisted breeding. In this paper, the seeds of S. bicolor × S. sudanense were treated with different concentration of NaCl and Na2CO3 stress, and the germination rates of the seeds were analyzed under different concentrations. The seedlings were treated with 200 mmol/L NaCl and Na2CO3 stress. The physiological indexes of soluble sugar, proline(PRO), catalase(CAT), peroxidase(POD)and total superoxide dismutase(T-SOD)were determined and transcriptome expression was analyzed in different time periods. The results showed that the germination rate and root length of S. bicolor × S. sudanense under the same concentration of neutral salt stress were higher than those under alkaline salt stress. With the time prolonging, POD and CAT showed a gradually decreasing trend under neutral salt stress, but gradually increasing trend under alkaline salt stress. PRO, soluble sugar and T-SOD increased gradually under neutral salt stress, but decreased gradually under alkaline salt stress. Transcriptome analysis showed that 241, 293 and 149 DEGs were identified after 6, 12 and 24 h of neutral salt stress, and 664, 641 and 728 DEGs were identified under alkaline salt stress. GO and KEGG cluster analysis revealed that DEGs involved in oxidative reductase synthesis, osmotic stress, cell membrane composition, cell oxidation and detoxification played a key role in the responses to salt-alkali stress the grass in the seedling. DEG mainly focused on hormone signal transduction, photosynthetic metabolism, redox, glucose metabolism, nucleic acid repair, phenylpropane biosynthesis and other processes related to abiotic stress or stress. Under saline-alkali stress, the grass seedlings responded to environmental stimuli through hormone signal transduction and oxidoreductase detoxification. Glucose metabolism and reductase synthesis and transport play an important role in salt-tolerant varieties.

Key words: Sorghum bicolor × S. sudanense, salt-alkali stress, transcriptome