Biotechnology Bulletin ›› 2023, Vol. 39 ›› Issue (9): 192-201.doi: 10.13560/j.cnki.biotech.bull.1985.2023-0258

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In Situ Screening of Carotenoid Degrading Strains and the Application in Improving Quality and Aroma of Cigar

WU Qiao-yin1,2,3(), SHI You-zhi4, LI Lin-lin4, PENG Zheng1,3, TAN Zai-yu4, LIU Li-ping4, ZHANG Juan1,3(), PAN Yong4()   

  1. 1. Key Laboratory of Industrial Biotechnology of Ministry of Education, Jiangnan University, Wuxi 214122
    2. School of Biotechnology, Jiangnan University, Wuxi 214122
    3. Science Center for Future Foods, Jiangnan University, Wuxi 214122
    4. China Tobacco Hubei Industry Co., Ltd., Wuhan 430040
  • Received:2023-03-21 Online:2023-09-26 Published:2023-10-24
  • Contact: ZHANG Juan, PAN Yong E-mail:6200201045@stu.jiangnan.edu.cn;zhangj@jiangnan.edu.cn;sx_panyong@sx.hbtobacoo.cn

Abstract:

Endophyte degrading carotenoids were screened and applied to domestic cigar leaves to increase domestic cigar tobacco leaves(CTLs)aroma and improve sensory quality. CTLs powder was added into in situ medium to simulate the growth environment of tobacco endophytes, and enrichment culture combined with flow cytometry technology was used to screen strains with β-carotene-degrading ability from high-quality cigar tobacco endophytes. The strains inoculated into domestic CTLs, and the contents of carotenoid degradation products were detected and the sensory quality of CTLs was analyzed. Total 21 endophytes had β-carotene degradation rate over 50%, mainly including Agrobacterium, Rhizobium, Sphingobacterium, Enterobacter, etc. These strains made different effects on CTLs sensory quality, among which strains C31, C11 and H4 increased the aroma type, significantly improved the aroma quality, aroma amount, miscellaneous gas, irritation and other CTLs sensory index. The carotenoid-degrading product content from strain C31, C11 and H4 CTLs was 1.38, 1.28 and 1.43 times compared with CK, respectively. The farnesyl acetone, geranyl acetone and megastigmatrienone strengthened the burnt sweet, flowery and woody aroma of CTLs. The three endophytes C31, C11 and H4 obtained by in situ screening can better improve the domestic CTLs sensory quality, and can be further developed and applied as microbial preparations of new cigar industry and characteristic aromatic cigar.

Key words: carotenoids, degradation, endophyte, situ screening, cigar tobacco leaves, fermentation, quality improvement, aroma enhancement