Isolation, Identification and Degradation Characteristics of Functional Bacteria for NH3 and H2S Degradation

doi:10.13560/j.cnki.biotech.bull.1985.2022-1202

Abstract

Abstract:

In order to reduce the emission of malodorous gas during the stacking of kitchen waste, two strains CN5 and CS2 with high degradation of NH₃ and H₂S were isolated and screened from kitchen waste by enrichment and domestication, qualitative primary screening and quantitative secondary screening methods. They were identified as Enterococcus sp. and Pseudomonas sp. by 16S rDNA sequence analysis. The optimal deodorizing conditions of microbial deodorant were determined by single factor experiment: culture temperature was 35℃, pH was 7, carbon nitrogen ratio was 25∶1, carbon source was sucrose, nitrogen source was ammonium chloride, and the degradation rate of NH₃ and H₂S could be maintained at about 85%. Strains CN5 and CS2 grew best in the mixed state of 2∶3, and the maximum value of OD₆₀₀ was 1.342. The determination of ammonia nitrogen content and sulfate content, it was found that the final ammonia nitrogen content was 50.5 mg/L, and the degradation rate of bacteria to ammonia nitrogen was 85.3%. The sulfate content was 302.7 mg/L, and the sulfate production rate of bacterial agent was 89.2%. The results showed that the two strains screened from the kitchen waste had good ability to degrading NH₃ and H₂S, and the two strains had synergistic effect with each other.

Key words: NH₃ degrading bacteria, H₂S degrading bacteria, screening, identification, optimization

JIANG Hai-rong, CUI Ruo-qi, WANG Yue BAI, Miao ZHANG, Ming-lu , REN Lian-hai. Isolation, Identification and Degradation Characteristics of Functional Bacteria for NH₃ and H₂S Degradation[J]. Biotechnology Bulletin, 2023, 39(9): 246-254.

Figures/Tables 10

Table 1 Experiment design of optimal deodorizing conditions

测试项目Test item	接种量Inoculation amount/%
测试项目Test item	5	10	15	20	—	—	—
pH	6.0	6.5	7.0	7.5	8.0	8.5	—
C/N	1∶15	1∶20	1∶25	1∶30	1∶35	—	—
碳源 Carbon source	无水葡萄糖 Anhydrous glucose	柠檬酸钠 Sodium citrate	乙酸钠 Sodium acetate	蔗糖 Sucrose	丁二酸钠 Sodium succinate	—	—
氮源 Nitrogen source	尿素 Carbamide	氯化铵 Ammonium chloride	硫酸铵 Ammonium sulphate	酵母提取物 Yeast extract	蛋白胨 Peptone	硝酸钾 Potassium nitrate	亚硝酸 Nitrous acid

Table 2 Screening results and degradation rates

NH₃降解菌株编号NH₃ degrading strain	降解率Degradation rate/%	H₂S降解菌株编号H₂S degrading strain	降解率Degradation rate/%
CN10	71.72	CS2	70.66
CN5	77.75	CS4	62.30
PN1	65.75	CS6	66.90
PN3	67.77	CS7	60.32

Fig. 1 Transformation of ammonia nitrogen（A）and sulfate（B）by strains

Fig. 2 Morphological characteristics of strains（10×100） under microscope

Fig. 3 Phylogenetic tree derived from 16S rDNA sequences of the CN5 and CS2 strains

Fig. 4 OD600 value of strains cultured at different temperatures A: OD600 value of strain CN5; B: OD600 value of strain CS2

Fig. 5 Changes in degradation rates of strain CN5 and strain CS2 under different culture conditions

Fig. 6 Changes of OD600 under different mixing ratios of two strains

Fig. 7 Changes of pH under different mixing ratios of two strains

Fig. 8 Variation curve of ammonium nitrogen and sulfate ion concentration under mixed conditions of two strains

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Isolation, Identification and Degradation Characteristics of Functional Bacteria for NH₃ and H₂S Degradation

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