Biotechnology Bulletin ›› 2024, Vol. 40 ›› Issue (6): 260-270.doi: 10.13560/j.cnki.biotech.bull.1985.2023-1101
Previous Articles Next Articles
YU Xin-lei1(), HE Jie-wang2, LIN Guo-ping3, LI Jin-hai3, WANG Da-ai1, YUAN Yue-bin1, LIU Sheng-gao1, LI Zhi-hao1, TAO De-xin3
Received:
2023-11-22
Online:
2024-06-26
Published:
2024-06-24
YU Xin-lei, HE Jie-wang, LIN Guo-ping, LI Jin-hai, WANG Da-ai, YUAN Yue-bin, LIU Sheng-gao, LI Zhi-hao, TAO De-xin. Metabolome Difference Analysis of Fermented Cigar Tobacco Leaves in Summer and Winter[J]. Biotechnology Bulletin, 2024, 40(6): 260-270.
Fig. 3 Overview of global metabolic profiles in fermented cigar tobacco samples of summer and winter A: Metabolites content categories. B: Clustering analysis. C: Principal component analysis score. D: Principal component analysis scatter(X: First principal component;Y: Second principal component). SF: Tobacco fermentation in summer. CK:Tobacco before fermentation. WF: Tobacco fermentation in winter. QC: Quality control sample. The same below
Fig. 4 Comparison of metabolite ion intensity in the fermented cigar tobacco leaves of summer and winter Different lowercase letters indicate Tukey HSD multiple comparison exists significant differences at the P< 0.05 level
编号 No. | 代谢通路 Metabolic pathway | 关联代谢物 Associated metabolites | 通路ID Pathway ID |
---|---|---|---|
1 | 代谢途径 Metabolic pathways | 胍丁胺、N-甲基-5-羟基色胺、顺式-5'-(2-氧代丙基)-烟碱、2-苯乙胺、去甲基烟碱、苯基乙醇胺、烟胺、3-吲哚乙腈 | ko01100 |
2 | 甘氨酸、丝氨酸和苏氨酸代谢 Glycine, serine and threonine metabolism | 乙酸胍 | ko00260 |
3 | 半胱氨酸和甲硫氨酸代谢 Cysteine and methionine metabolism | 烟胺 | ko00270 |
4 | 精氨酸和脯氨酸代谢Arginine and proline metabolism | 胍丁胺、N-乙酰腐胺、阿魏酰腐胺 | ko00330 |
5 | 酪氨酸代谢Tyrosine metabolism | L-酪胺 | ko00350 |
6 | 苯丙氨酸代谢 Phenylalanine metabolism | 2-苯乙胺 | ko00360 |
7 | 色氨酸代谢 Tryptophan metabolism | N-甲基-5-羟基色胺、甲氧基吲哚乙酸、3-吲哚乙腈 | ko00380 |
8 | 核黄素代谢Riboflavin metabolism | 7,8-二甲基咯嗪 | ko00740 |
9 | 烟酸和烟酰胺代谢Nicotinate and nicotinamide metabolism | 1,4-二氢-1-甲基-4-氧代-3-吡啶甲酰胺、烟酸甲酯 | ko00760 |
10 | 异喹啉生物碱生物合成Isoquinoline alkaloid biosynthesis | L-酪胺 | ko00950 |
11 | 莨菪烷、哌啶和吡啶生物碱生物合成 Tropane, piperidine and pyridine alkaloid biosynthesis | 去甲基烟碱 | ko00960 |
12 | 植物次生代谢产物的生物合成 Biosynthesis of various plant secondary metabolites | 烟胺、3-吲哚乙腈、阿魏酰腐胺、1-甲基胍、对香豆酰酪胺 | ko00999 |
Table 1 Metabolic pathways associated with differential alkaloids
编号 No. | 代谢通路 Metabolic pathway | 关联代谢物 Associated metabolites | 通路ID Pathway ID |
---|---|---|---|
1 | 代谢途径 Metabolic pathways | 胍丁胺、N-甲基-5-羟基色胺、顺式-5'-(2-氧代丙基)-烟碱、2-苯乙胺、去甲基烟碱、苯基乙醇胺、烟胺、3-吲哚乙腈 | ko01100 |
2 | 甘氨酸、丝氨酸和苏氨酸代谢 Glycine, serine and threonine metabolism | 乙酸胍 | ko00260 |
3 | 半胱氨酸和甲硫氨酸代谢 Cysteine and methionine metabolism | 烟胺 | ko00270 |
4 | 精氨酸和脯氨酸代谢Arginine and proline metabolism | 胍丁胺、N-乙酰腐胺、阿魏酰腐胺 | ko00330 |
5 | 酪氨酸代谢Tyrosine metabolism | L-酪胺 | ko00350 |
6 | 苯丙氨酸代谢 Phenylalanine metabolism | 2-苯乙胺 | ko00360 |
7 | 色氨酸代谢 Tryptophan metabolism | N-甲基-5-羟基色胺、甲氧基吲哚乙酸、3-吲哚乙腈 | ko00380 |
8 | 核黄素代谢Riboflavin metabolism | 7,8-二甲基咯嗪 | ko00740 |
9 | 烟酸和烟酰胺代谢Nicotinate and nicotinamide metabolism | 1,4-二氢-1-甲基-4-氧代-3-吡啶甲酰胺、烟酸甲酯 | ko00760 |
10 | 异喹啉生物碱生物合成Isoquinoline alkaloid biosynthesis | L-酪胺 | ko00950 |
11 | 莨菪烷、哌啶和吡啶生物碱生物合成 Tropane, piperidine and pyridine alkaloid biosynthesis | 去甲基烟碱 | ko00960 |
12 | 植物次生代谢产物的生物合成 Biosynthesis of various plant secondary metabolites | 烟胺、3-吲哚乙腈、阿魏酰腐胺、1-甲基胍、对香豆酰酪胺 | ko00999 |
样品 Sample | 香气质 Aroma quality | 香气量 Aroma quantity | 杂气 Disorder | 刺激性 Thrill | 余味 Aftertaste | 甜度 Sweetness | 燃烧性 Inflammability | 灰色 Grayness | 合计 Total |
---|---|---|---|---|---|---|---|---|---|
发酵初始样 | 12 | 12 | 6 | 7.5 | 8 | 4 | 8 | 7.5 | 65 |
冬季发酵叶 | 13 | 12 | 7 | 8 | 9 | 6.5 | 8.5 | 8 | 72 |
夏季发酵叶 | 15 | 13 | 7.5 | 9 | 10 | 7 | 8.5 | 8 | 78 |
Table 2 Sensory quality of fermented cigar tobacco leaves from summer and winter
样品 Sample | 香气质 Aroma quality | 香气量 Aroma quantity | 杂气 Disorder | 刺激性 Thrill | 余味 Aftertaste | 甜度 Sweetness | 燃烧性 Inflammability | 灰色 Grayness | 合计 Total |
---|---|---|---|---|---|---|---|---|---|
发酵初始样 | 12 | 12 | 6 | 7.5 | 8 | 4 | 8 | 7.5 | 65 |
冬季发酵叶 | 13 | 12 | 7 | 8 | 9 | 6.5 | 8.5 | 8 | 72 |
夏季发酵叶 | 15 | 13 | 7.5 | 9 | 10 | 7 | 8.5 | 8 | 78 |
[1] | 李爱军, 秦艳青, 代惠娟, 等. 国产雪茄烟叶科学发展刍议[J]. 中国烟草学报, 2012, 18(1): 112-114. |
Li AJ, Qin YQ, Dai HJ, et al. On scientific development of China's cigar leaf[J]. Acta Tabacaria Sin, 2012, 18(1): 112-114. | |
[2] | 寇明钰, 汪长国, 戴亚, 等. 不同产地和等级的雪茄烟叶中游离氨基酸含量分析[J]. 西南农业学报, 2013, 26(3): 963-967. |
Kou MY, Wang CG, Dai Y, et al. Analysis on free amino in cigar tobacco of different habitats and grades[J]. Southwest China J Agric Sci, 2013, 26(3): 963-967. | |
[3] | 时向东, 汪文杰, 王卫武, 等. 遮荫下氮肥用量对雪茄外包皮烟叶光合特性的调控效应[J]. 植物营养与肥料学报, 2007, 13(2): 299-304. |
Shi XD, Wang WJ, Wang WW, et al. Response of photosynthetic characteristics in leaves of cigar wrapper tobacco to nitrogen application under shady condition[J]. J Plant Nutr Fertil, 2007, 13(2): 299-304. | |
[4] | 徐世杰, 王洁, 王慧方, 等. 调制过程中不同温湿度条件对海南雪茄茄衣烟叶质量的影响[J]. 山东农业科学, 2016, 48(1): 29-34. |
Xu SJ, Wang J, Wang HF, et al. Effects of temperature and humidity on cigar- wrapper tobacco quality in Hainan Province during curing[J]. Shandong Agric Sci, 2016, 48(1): 29-34. | |
[5] | 蔡斌, 耿召良, 高华军, 等. 国产雪茄原料生产技术研究现状[J]. 中国烟草学报, 2019, 25(6): 110-119. |
Cai B, Geng ZL, Gao HJ, et al. Research progress of production technologies of cigar tobaccos in China[J]. Acta Tabacaria Sin, 2019, 25(6): 110-119. | |
[6] | 张倩颖, 罗诚, 李东亮, 等. 雪茄烟叶调制及发酵技术研究进展[J]. 中国烟草学报, 2020, 26(4): 1-6. |
Zhang QY, Luo C, Li DL, et al. Research progress in curing and fermentation technology for cigar tobacco leaf production[J]. Acta Tabacaria Sin, 2020, 26(4): 1-6. | |
[7] | 张鸽, 辛玉华, 王娟. 雪茄外包皮烟叶发酵研究进展[C]// 中国烟草学会学术年会优秀论文集. 北京: 中国烟草学会, 2017: 15. |
Zhang G, Xin YH, Wang J, et al. Research advancement of cigar wrapper fermentation[C]// Collection of Excellent Papers at the Academic Annual Meeting of China Tobacco Society. Beijing: China Tobacco Society, 2017: 15. | |
[8] | 雷云康, 谭舒, 曾旸, 等. 德阳雪茄烟叶的农业发酵研究[J]. 智慧农业导刊, 2022, 2(6): 39-41. |
Lei YK, Tan S, Zeng Y, et al. Study on agricultural fermentation of Deyang cigar tobacco[J]. J Smart Agric, 2022, 2(6): 39-41. | |
[9] | 潘勇, 王剑, 李林林, 等. 雪茄优势菌株接种发酵对其烟叶品质的影响[J]. 贵州农业科学, 2023, 51(12): 125-132. |
Pan Y, Wang J, Li LL, et al. Effects of inoculation and fermentation of cigar dominant strains on quality of cigar leaves[J]. Guizhou Agric Sci, 2023, 51(12): 125-132. | |
[10] | 李凌, 章存勇. 不同物料处理对雪茄烟叶发酵后香气成分和抽吸质量的影响[J]. 南方农业, 2016, 10(3): 254-256. |
Li L, Zhang CY. Effects of different material treatments on aroma components and smoking quality of cigar tobacco leaves after fermentation[J]. South China Agric, 2016, 10(3): 254-256. | |
[11] | 郭文龙. 茄芯烟叶发酵过程中颜色参数变化及外源加酶发酵对其质量的影响[D]. 郑州: 河南农业大学, 2022. |
Guo WL. Changes of color parameters during fermentation of eggplant core tobacco leaves and effects of exogenous enzyme fermentation on their quality[D]. Zhengzhou: Henan Agricultural University, 2022. | |
[12] | 杨春雷, 李宗平, 徐恒, 等. 一种雪茄烟茄芯烟叶双称重回潮方法: CN113133539B[P]. 2022-06-21. |
Yang CL, Li ZP, Xu H, et al. Double-weighing moisture regaining method for cigar core tobacco leaves: CN113133539B[P]. 2022-06-21. | |
[13] | 国家质量监督检验检疫总局, 中国国家标准化管理委员会. 雪茄烟第4部分:感官技术要求: GB 15269.4—2011[S]. 北京: 中国标准出版社, 2012. |
General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China. Cigars—Part 4: Technical requirements for sense evaluation: GB 15269.4—2011[S]. Beijing: Standards Press of China, 2012. | |
[14] | 谭绍安, 田煜利, 刘学兵, 等. 雪茄烟叶加湿堆积发酵技术研究与应用[J]. 安徽农业科学, 2017, 45(23): 80-83. |
Tan SA, Tian YL, Liu XB, et al. Research and application of humidifying and stacking fermentation technology of cigar tobacco[J]. J Anhui Agric Sci, 2017, 45(23): 80-83. | |
[15] | 乔保明, 田煜利, 刘学兵, 等. 发酵温度对雪茄烟茄芯烟叶质量影响分析[J]. 中国科技信息, 2018(8): 39-40. |
Qiao BM, Tian YL, Liu XB, et al. Effect of fermentation temperature on the quality of eggplant core leaves of cigars[J]. China Sci Technol Inf, 2018(8): 39-40. | |
[16] | 虞昕磊, 袁跃斌, 饶熊飞, 等. 发酵温度对茄芯烟叶化学成分和感官品质的影响[J]. 中南农业科技, 2023, 44(1): 20-23. |
Yu XL, Yuan YB, Rao XF, et al. Effect of fermentation temperature on the chemical composition and sensory quality of aubergine core tobacco leaves[J]. South-Central Agricultural Science and Technology, 2023, 44(1): 20-23. | |
[17] | 胡婉蓉, 蔡文, 郑召君, 等. 发酵介质和发酵过程对雪茄烟叶品质的影响[J]. 烟草科技, 2023, 56(2): 41-52. |
Hu WR, Cai W, Zheng ZJ, et al. Effects of fermentation media and processes on cigar tobacco quality[J]. Tob Sci Technol, 2023, 56(2): 41-52. | |
[18] | 刘立全, 王月霞. 梅拉德反应在烟草增香中的应用研究进展[J]. 烟草科技, 1994, 27(6): 21-24. |
Liu LQ, Wang YX. Research progress on the application of Maillard reaction in tobacco flavoring[J]. Tob Sci Technol, 1994, 27(6): 21-24. | |
[19] | 刘路路, 郭文龙, 胡希, 等. 雪茄烟叶发酵过程中颜色变化与主要含氮化合物及评吸质量的关系[J]. 南方农业学报, 2022, 53(2): 497-504. |
Liu LL, Guo WL, Hu X, et al. The relationship between the color change of cigar tobacco leaf and the main nitrogen compounds and smoking quality during fermentation[J]. J South Agric, 2022, 53(2): 497-504. | |
[20] | 周迪, 孙雨琦, 史宏志, 等. 16个雪茄烟品种的烟碱转化率及与发酵后烟叶TSNAs含量的关系[J]. 中国烟草学报, 2020, 26(4): 18-25. |
Zhou D, Sun YQ, Shi HZ, et al. Nicotine conversion rate of 16 cigar varieties and its relationship with TSNAs content in fermented tobacco leaves[J]. Acta Tabacaria Sin, 2020, 26(4): 18-25. | |
[21] | 杜咏梅, 郭承芳, 张怀宝, 等. 水溶性糖、烟碱、总氮含量与烤烟吃味品质的关系研究[J]. 中国烟草科学, 2000, 21(1): 7. |
Du YM, Guo CF, Zhang HB, et al. Study on relationship between content of water soluble sugar, alkaloid, total nitrogen and taste quality of flue cured tobacco[J]. Chin Tob Sci, 2000, 21(1): 7. | |
[22] | 韦凤杰, 刘国顺, 杨永锋, 等. 烤烟成熟过程中类胡萝卜素变化与其降解香气物质关系[J]. 中国农业科学, 2005, 38(9): 1882-1889. |
Wei FJ, Liu GS, Yang YF, et al. Relations between cartenoids and aromatic components from the cartenoids in flue-cured tobacco(Nicotinna tobacum L.) leaves at different mature periods[J]. Sci Agric Sin, 2005, 38(9): 1882-1889. | |
[23] | 李晶晶, 史宏志, 杨兴有, 等. 发酵条件对雪茄芯叶TSNAs及其前体物含量变化的影响[J]. 中国烟草学报, 2019, 25(6): 120-125. |
Li JJ, Shi HZ, Yang XY, et al. Effects of fermentation conditions on contents of TSNAs and their precursors in cigar filler tobacco[J]. Acta Tabacaria Sin, 2019, 25(6): 120-125. | |
[24] | 荣仕宾, 李晶晶, 赵园园, 等. 雪茄芯叶人工发酵温湿度控制对化学成分和香味品质的影响[J]. 中国烟草学报, 2021, 27(2): 109-116. |
Rong SB, Li JJ, Zhao YY, et al. Effect of artificial fermentation temperature and humidity on chemical composition and aroma quality of cigar filler tobacco[J]. Acta Tabacaria Sin, 2021, 27(2): 109-116. | |
[25] | 虞昕磊, 袁跃斌, 饶熊飞, 等. 烟叶回潮含水率对雪茄茄芯发酵品质的影响[J]. 农技服务, 2023, 40(3): 59-65. |
Yu XL, Yuan YB, Rao XF, et al. The effect of moisture content of tobacco leaves on the fermentation quality of cigar fillers[J]. Agric Technol Serves, 2023, 40(3): 59-65. |
[1] | YANG Lu, YUAN Yuan, FANG Zhi-kai, LIN Ru, JIANG Hong, ZHOU Jian. Identification of a Streptomyces Strain and Study on the Fermentation Process of Geldanamycin Production [J]. Biotechnology Bulletin, 2024, 40(6): 299-309. |
[2] | ZHANG Mei-yu, ZHAO Yu-bin, WANG Ling-yun, SONG Yuan-da, ZHAO Xin-he, REN Xiao-jie. Research Progress in the Production of Functional Fatty Acid DHA by Microalga Thraustochytrids [J]. Biotechnology Bulletin, 2024, 40(6): 81-94. |
[3] | GUAN Zhi-jing, SUN Chao. Research Progress in the Compartmentalization of Plant Specialized Metabolism [J]. Biotechnology Bulletin, 2024, 40(1): 1-11. |
[4] | ZHANG Jin-wei, WU Yuan-xia, SUN Jing, LI Xiao-kai, LU Lu, LI Zhou-quan, GE Liang-peng. Effects of Commensal Microbiota on Intestinal Development, Metabolism, and Mitochondrial Function in Piglets [J]. Biotechnology Bulletin, 2024, 40(1): 332-343. |
[5] | LI Liang, XU Shan-shan, JIANG Yan-jun. Research Progress in the Production of Ergothioneine by Biosynthesis [J]. Biotechnology Bulletin, 2024, 40(1): 86-99. |
[6] | WU Qiao-yin, SHI You-zhi, LI Lin-lin, PENG Zheng, TAN Zai-yu, LIU Li-ping, ZHANG Juan, PAN Yong. In Situ Screening of Carotenoid Degrading Strains and the Application in Improving Quality and Aroma of Cigar [J]. Biotechnology Bulletin, 2023, 39(9): 192-201. |
[7] | MIAO Yong-mei, MIAO Cui-ping, YU Qing-cai. Properties of Bacillus subtilis Strain BBs-27 Fermentation Broth and the Inhibition of Lipopeptides Against Fusarium culmorum [J]. Biotechnology Bulletin, 2023, 39(9): 255-267. |
[8] | XU Fa-di, XU Kang, SUN Dong-ming, LI Meng-lei, ZHAO Jian-zhi, BAO Xiao-ming. Research Progress in Second-generation Fuel Ethanol Technology Based on Poplar(Populus sp.) [J]. Biotechnology Bulletin, 2023, 39(9): 27-39. |
[9] | ZHANG Yue-yi, LAN She-yi, PEI Hai-run, FENG Di. Process Optimization of Multi-strain Fermented Oat Bran and Hair Efficacy Evaluation [J]. Biotechnology Bulletin, 2023, 39(9): 58-70. |
[10] | CHENG Ya-nan, ZHANG Wen-cong, ZHOU Yuan, SUN Xue, LI Yu, LI Qing-gang. Synthetic Pathway Construction of Producing 2'-fucosyllactose by Lactococcus lactis and Optimization of Fermentation Medium [J]. Biotechnology Bulletin, 2023, 39(9): 84-96. |
[11] | ZHAO Si-jia, WANG Xiao-lu, SUN Ji-lu, TIAN Jian, ZHANG Jie. Modification of Pichia pastoris for Erythritol Production by Metabolic Engineering [J]. Biotechnology Bulletin, 2023, 39(8): 137-147. |
[12] | ZHENG Huan, LIN Dong-mei, LIU Jun-yuan, ZHANG Yin-lian, LIN Biao-sheng, LIN Zhan-xi, LI Jing. Analysis of Amino Acid Metabolism Difference Between Fruiting Body and Mycelium of Taiwanofungus camphoratus by LC-QTOF-MS Metabonomics [J]. Biotechnology Bulletin, 2023, 39(5): 254-266. |
[13] | YAO Jin-dong, TANG Hua-mei, YANG Wen-xiao, ZHANG Li-shan, LIN Xiang-min. Comparative Proteomics Analysis of Aeromonas hydrophila Under Enrofloxacin Stress [J]. Biotechnology Bulletin, 2023, 39(4): 288-296. |
[14] | CUI Ji-jie, CAI Wen-bo, ZHUANG Qing-hui, GAO Ai-ping, HUANG Jian-feng, CHEN Ya-hui, SONG Zhi-zhong. Biological Function of Gene MiISU1 for Fe-S Cluster Assembly in Mangifera indica [J]. Biotechnology Bulletin, 2023, 39(2): 139-146. |
[15] | LI Xin-yue, ZHOU Ming-hai, FAN Ya-chao, LIAO Sha, ZHANG Feng-li, LIU Chen-guang, SUN Yue, ZHANG Lin, ZHAO Xin-qing. Research Progress in the Improvement of Microbial Strain Tolerance and Efficiency of Biological Manufacturing Based on Transporter Engineering [J]. Biotechnology Bulletin, 2023, 39(11): 123-136. |
Viewed | ||||||
Full text |
|
|||||
Abstract |
|
|||||