Genome-wide Identification and Expression Analysis of bZIP Transcription Factor Family in Dendrobium officinale

doi:10.13560/j.cnki.biotech.bull.1985.2025-0094

Abstract

Abstract:

Objective To identify the bZIP gene family of Dendrobium officinale,and clarify the biological function of DobZIP transcription factors through bioinformatics and gene expression analysis, thus aiming to provide theoretical guidance for further exploration of the molecular mechanism of DobZIP transcription factors. Method Based on the genome data of Dendrobium officinale in the IMP database, we identified the members of DobZIP gene family, and performed multiple of bioinformatics analysis. Furthermore, we used transcriptome data and real-time fluorescent quantitative PCR technology to analyze the tissue expression pattern and abiotic stress expression of DobZIP transcription factors. Result A total of 54 DobZIP genes were identified. None of the DobZIP proteins had signal peptides, and only DobZIP54 had a transmembrane domain. The subcellular localization of the DobZIP proteins were mainly in the nucleus. Members of the DobZIP family were classified into 10 subfamilies, and members of the same subfamily contained similar structures and motifs. DobZIP genes were unevenly distributed on 18 chromosomes, with two pairs of genes showing tandem relationships. There were multiple collinear relationships between species, and DobZIP family had a high homology with the OsbZIP family in rice. Cis-acting elements analysis indicated that the DobZIP family members all contained multiple cis-acting elements involved in growth and development and response to abiotic stress. Gene expression pattern analysis showed that the DobZIP family members had tissue-specific expression, and DobZIP4、DobZIP10、DobZIP28、DobZIP32 and DobZIP37 were induced by cold, drought and NaCl stress to varying degrees. In addition to DobZIP32 and DobZIP37, DobZIP4, DobZIP10 and DobZIP28 were also induced by high temperature stress. Conclusion A total of 54 DobZIP genes are identified in the genome of Dendrobium officinale. DobZIP genes was in tissue-specific expression, and DobZIP4, DobZIP10, DobZIP28, DobZIP32 and DobZIP37 are induced to varying degrees under cold, drought, and NaCl stress.

Key words: Dendrobium officinale, transcription factor, bZIP, bioanalysis, gene expression analysis

ZENG Dan, HUANG Yuan, WANG Jian, ZHANG Yan, LIU Qing-xia, GU Rong-hui, SUN Qing-wen, CHEN Hong-yu. Genome-wide Identification and Expression Analysis of bZIP Transcription Factor Family in Dendrobium officinale[J]. Biotechnology Bulletin, 2025, 41(8): 197-210.

Figures/Tables 12

Table 1 Primer sequences for quantitative PCR

引物名称 Primer name	引物序列Primer sequence（5'-3'）
DobZIP4-F	GAATCCGTCGTCTGAGTC
DobZIP4-R	TGTCGCTGGTATGGTTATC
DobZIP10-F	AGAGGATAGCGATGAGGAT
DobZIP10-R	TGTGGCTGTGGATTCAAG
DobZIP28-F	GGTCTGTAAGTGATTCTCTGA
DobZIP28-R	CGCCTTCTTGATGTTCTCT
DobZIP32-F	TCTCAAGGTATGGAAGCATT
DobZIP32-R	AGATTGTCGTGTCGTTAGTA
DobZIP37-F	ACCAGAAGAAGAGGAAGAGA
DobZIP37-R	GGATTGATGAGGAGGAGTTC
DoActin-F	TCCCAAGGCAAACAGAGAAA
DoActin-R	GGCCACTAGCATATAGGGAAAG

Fig. 1 Phylogenetic tree analysis of bZIP gene family in D. officinale and Arabidopsis thaliana

Fig. 2 Multiple alignment of D. officinale bZIP family protein sequencesThe red box indicates the conserved domain of the bZIP family protein of D. officinale

Fig. 3 Conserved motif analysis of bZIP family in D.officinale

Fig. 4 Protein domain analysis of bZIP family in D.officinale

Fig. 5 Gene structure analysis of bZIP family in D.officinale

Fig. 6 Chromosome localization analysis of bZIP family in D. officinale

Fig. 7 Promoter cis-acting elements analysis of bZIP members family in D. officinale

Fig. 8 Chromosomal distribution and collinearity analysis of bZIP transcription factors in D. officinale

Fig. 9 Synteny analysis of bZIP transcription factors between D.officinale, Oryza sativa and Glycine max

Fig. 10 Expression analysis of DobZIP transcription factorsin the roots, stems and leaves of D. officinale

Fig. 11 Relative expression analysis of five bZIP transcription factors under stress treatment in D.officinale

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