Genome-wide Identification of GATA Gene Family of Jindou Kumquat （Fortunella hindsii） and Their Expression Analysis in Fruit Development

doi:10.13560/j.cnki.biotech.bull.1985.2024-0880

Abstract

Abstract:

Objective To explore the regulation of GATA transcription factor gene family in the development of Jindou Kumquat (Fortunella hindsii). Method Bioinformatics was used to identify its GATA transcription factor gene family, and analyze its physicochemical properties, gene structure, phylogenetic tree and expression patterns via combining transcriptome data with RT-qPCR. Result A total of 26 FhGATAs transcription factors were identified in Jindou Kumquat, unevenly distributed on the nine chromosomes. Analysis of the physicochemical properties showed that, this family of proteins were all located in the nucleus, molecular weight in the range from 18 019.89 to 60 342.13 Da, belonging to the hydrophilic proteins, and the pI were 4.62 to 10.33. Cluster analysis with orthologous genes in Arabidopsis thaliana, FhGATA gene family can be divided into four subfamilies, the motif composition and distribution patterns were similar in the same subfamily, and some genes in the Subfamily Ⅲ were missing during evolution. Collinearity analysis showed that, 7 collinear FhGATAs genes in Jindou Kumquat, 24 AtGATAs gene pairs were homologous to the FhGATA gene in A. thaliana, 33 CsGATAs gene were homologous to FhGATA gene in Citrus sinensis. The mechanism of transcriptional regulation of FhGATA genes contained reactive elements that were light-responsive and light, and various hormone responses, stress responses, and growth and development-related. Tissue expression analysis revealed that, the four subfamilies of the FhGATAs gene family showed varying degrees of specific expression in the root, stem, leaf, flower, fruit and seed; moreover, FhGATA24, FhGATA22, FhGATA26, FhGATA18, FhGATA12 and FhGATA5 were highly expressed in the fruit. Further qRT-PCR analysis indicated that, the FhGATA gene was closely related to the single fruit weight, horizontal diameter and vertical diameter of Jindou Kumquat, among them, FhGATA22 and FhGATA18 showed significant differences in the whole fruit development of Jindou kumquat. Conclusion The Jindou kumquat contains 26 FhGATAs genes, of which FhGATA22 and FhGATA18 may be regulators mediating the fruit size.

Key words: Fortunella hindsii, GATA, transcription factor, gene family, fruit development, expression analysis

LUO Si-fang, ZHANG Zu-ming, XIE Li-fang, GUO Zi-jing, CHEN Zhao-xing, YANG Yue-hua, YAN Xiang, ZHANG Hong-ming. Genome-wide Identification of GATA Gene Family of Jindou Kumquat （Fortunella hindsii） and Their Expression Analysis in Fruit Development[J]. Biotechnology Bulletin, 2025, 41(5): 218-230.

Figures/Tables 10

References 40

1	胡红雨, 鲁子贤. “锌指” 结构: 蛋白质和DNA相互作用的一种模式 [J]. 生物化学与生物物理进展, 1992, 19(4): 250-253.
	Hu HY, Lu ZX. “Zinc finger” structure: a mode of interaction between protein and DNA [J]. Prog Biochem Biophys, 1992, 19(4): 250-253.
2	朱本章. Zfhag的锌指结构和同结构域的基因重组、表达和功能研究 [J]. 西安医科大学学报, 1996(3): 271-274.
	Zhu BZ. Studies on the recombination and expression of the zinc-finger homeodomain genes of zfhag and the function of the expressed products [J]. J Xi'an Med Univ Chin, 1996(3): 271-274.
3	周畅, 李麓芸. C₂H₂型锌指蛋白的研究进展 [J]. 生命科学研究, 2004, 8(3): 215-220.
	Zhou C, Li LY. Advances in C₂H₂ zinc finger proteins [J]. Life Sci Res, 2004, 8(3): 215-220.
4	史莹华, 许梓荣. GATA转录因子研究进展 [J]. 生物学通报, 2005, 40(3): 1-2.
	Shi YH, Xu ZR. Recent advances in GATA transcription factor [J]. Bull Biol, 2005, 40(3): 1-2.
5	田春艳, 张令强, 贺福初. KRAB型锌指蛋白(KZNF)的研究进展 [J]. 遗传, 2006, 28(11): 1451-1456.
	Tian CY, Zhang LQ, He FC. Progress in the study of KRAB zinc finger protein [J]. Hereditas, 2006, 28(11): 1451-1456.
6	Shi M, Huang QK, Wang Y, et al. Genome-wide survey of the GATA gene family in camptothecin-producing plant Ophiorrhiza pumila [J]. BMC Genomics, 2022, 23(1): 256.
7	Kim M, Xi H, Park J. Genome-wide comparative analyses of GATA transcription factors among 19 Arabidopsis ecotype genomes: Intraspecific characteristics of GATA transcription factors [J]. PLoS One, 2021, 16(5): e0252181.
8	Lai DL, Yao X, Yan J, et al. Genome-wide identification, phylogenetic and expression pattern analysis of GATA family genes in foxtail millet (Setaria italica) [J]. BMC Genomics, 2022, 23(1): 549.
9	Liu X, Zhu XL, Wei XN, et al. The wheat LLM-domain-containing transcription factor TaGATA1 positively modulates host immune response to Rhizoctonia cerealis [J]. J Exp Bot, 2020, 71(1): 344-355.
10	Reyes JC, Isabel Muro-Pastor M, Florencio FJ. The GATA family of transcription factors in Arabidopsis and rice [J]. Plant Physiol, 2004, 134(4): 1718-1732.
11	Du X, Lu YX, Sun HC, et al. Genome-wide analysis of wheat GATA transcription factor genes reveals their molecular evolutionary characteristics and involvement in salt and drought tolerance [J]. Int J Mol Sci, 2022, 24(1): 27.
12	He FY, Duan SG, Jian YQ, et al. Genome-wide identification and gene expression analysis of the 14-3-3 gene family in potato (Solanum tuberosum L.) [J]. BMC Genomics, 2022, 23(1): 811.
13	Wang TT, Yang Y, Lou ST, et al. Genome-wide characterization and gene expression analyses of GATA transcription factors in moso bamboo (Phyllostachys edulis) [J]. Int J Mol Sci, 2019, 21(1): 14.
14	Chen TT, Peng J, Li MJ, et al. Genetic analysis of the grapevine GATA gene family and their expression profiles in response to hormone and downy mildew infection [J]. Horticulturae, 2022, 8(4): 303.
15	Zheng KH, Lu JY, He XY, et al. Genome-wide identification and expression analysis of GATA family genes in Dimocarpus longan lour [J]. Int J Mol Sci, 2024, 25(2): 731.
16	Fan J, Xian BH, Huang X, et al. Genome-wide identification and characterization of the sweet orange (Citrus sinensis) GATA family reveals a role for CsGATA12 as a regulator of Citrus bacterial canker resistance [J]. Int J Mol Sci, 2024, 25(5): 2924.
17	Schwechheimer C, Schröder PM, Blaby-Haas CE. Plant GATA factors: their biology, phylogeny, and phylogenomics [J]. Annu Rev Plant Biol, 2022, 73: 123-148.
18	Du K, Xia YF, Zhan DJ, et al. Genome-wide identification of the Eucalyptus urophylla GATA gene family and its diverse roles in chlorophyll biosynthesis [J]. Int J Mol Sci, 2022, 23(9): 5251.
19	Liang JH, Wu Z, Xu TF, et al. Overexpression of HANABA TARANU in cultivated strawberry delays flowering and leads to defective flower and fruit development [J]. Plant Sci, 2022, 321: 111307.
20	Nawy T, Bayer M, Mravec J, et al. The GATA factor HANABA TARANU is required to position the proembryo boundary in the early Arabidopsis embryo [J]. Dev Cell, 2010, 19(1): 103-113.
21	Wang T, Chen LL, Shu HJ, et al. Fortunella venosa (champ. Ex Benth.) C. C. Huang and F. Hindsii (champ. Ex Benth.) Swingle as independent species: evidence from morphology and molecular systematics and taxonomic revision of Fortunella (rutaceae) [J]. Front Plant Sci, 2022, 13: 867659.
22	Barreca D, Bellocco E, Caristi C, et al. Kumquat (Fortunella japonica Swingle) juice: flavonoid distribution and antioxidant properties [J]. Food Res Int, 2011, 44(7): 2190-2197.
23	李怀福, 胡小三. 山金柑实生苗童期的研究 [J]. 特产研究, 2005, 27(1): 23-25, 39.
	Li HF, Hu XS. The study on the young period of the seedling tree of Fortunella hindsii Swingle [J]. Spec Wild Econ Anim Plant Res, 2005, 27(1): 23-25, 39.
24	Song XT, Xie YY, Tian XY, et al. CRISPR/Cas9 editing characteristics of multiple transgenic generations in Fortunella hindsii, an early flowering mini-Citrus [J]. Sci Hortic, 2023, 321: 112236.
25	罗嗣芳, 张祖铭, 柳嘉程, 等. 赣南野生山金柑种质资源收集与鉴定 [J]. 植物遗传资源学报, 2024, 25(2):237-248.
	Luo SF, Zhang ZM, Liu JC, et al. Collection and identification of wild kumquat germplasm resources in Gannan [J]. J Plant Genet Resour, 2024, 25(2): 237-248.
26	罗艾, 龚桂芝, 彭祝春, 等. 柑橘果实大小与质量的遗传分析和数量性状位点定位 [J]. 浙江大学学报: 农业与生命科学版, 2021, 47(6): 719-728.
	Luo A, Gong GZ, Peng ZC, et al. Genetic analysis and quantitative trait locus mapping of Citrus fruit size and mass [J]. J Zhejiang Univ Agric Life Sci, 2021, 47(6): 719-728.
27	He W, Xie R, Li H, et al. Evaluation of suitable qRT-PCR normalization genes for various Citrus rootstocks [J]. Plant Biotechnol Rep, 2022, 16(1): 101-111.
28	肖翠, 严佳文, 龙桂友, 等. 柑橘内参基因的稳定性评价 [J]. 果树学报, 2012, 29(6): 978-984.
	Xiao C, Yan JW, Long GY, et al. Stability evaluation of reference genes in Citrus [J]. J Fruit Sci, 2012, 29(6): 978-984.
29	青亚林, 侯炳豪, 张宇航, 等. 茶树GATA基因家族的全基因组鉴定及表达分析 [J]. 江苏农业科学, 2024, 52(9): 42-50.
	Qing YL, Hou BH, Zhang YH, et al. Genome-wide identification and expression analysis of GATA gene family in Camellia sinensis [J]. Jiangsu Agric Sci, 2024, 52(9): 42-50.
30	Song XD, Wang YK, Wang P, et al. GATA-type transcriptional factor Gat1 regulates nitrogen uptake and polymalic acid biosynthesis in polyextremotolerant fungus Aureobasidium pullulans [J]. Environ Microbiol, 2020, 22(1): 229-242.
31	Feng X, Yu Q, Zeng JB, et al. Genome-wide identification and characterization of GATA family genes in wheat [J]. BMC Plant Biol, 2022, 22(1): 372.
32	Robert-Seilaniantz A, Grant M, Jones JDG. Hormone crosstalk in plant disease and defense: more than just jasmonate-salicylate antagonism [J]. Annu Rev Phytopathol, 2011, 49: 317-343.
33	Vision TJ, Brown DG, Tanksley SD. The origins of genomic duplications in Arabidopsis [J]. Science, 2000, 290(5499): 2114-2117.
34	Huang FY, Ye XW, Wang ZJ, et al. The prohibitins (PHB) gene family in tomato: Bioinformatic identification and expression analysis under abiotic and phytohormone stresses [J]. GM Crops Food, 2021, 12(1): 535-550.
35	Zhang LJ, Li XX, Ma B, et al. The Tartary buckwheat genome provides insights into rutin biosynthesis and abiotic stress tolerance [J]. Mol Plant, 2017, 10(9): 1224-1237.
36	Li LZ, He YJ, Ge HY, et al. Functional characterization of SmMYB86, a negative regulator of anthocyanin biosynthesis in eggplant (Solanum melongena L.) [J]. Plant Sci, 2021, 302: 110696.
37	Li SH, Dong YX, Li DL, et al. Eggplant transcription factor SmMYB5 integrates jasmonate and light signaling during anthocyanin biosynthesis [J]. Plant Physiol, 2024, 194(2): 1139-1165.
38	王娟, 兰海燕. GATA转录因子对植物发育和胁迫响应调控的研究进展 [J]. 植物生理学报, 2016, 52(12): 1785-1794.
	Wang J, Lan HY. Advances in regulation of GATA transcription factor to plant development and stress responses [J]. Plant Physiol J, 2016, 52(12): 1785-1794.
39	Wang YQ, Cao XY, Zhang DK, et al. SlGATA17, A tomato GATA protein, interacts with SlHY5 to modulate salinity tolerance and germination [J]. Environ Exp Bot, 2023, 206: 105191.
40	Wang WH, Wang PW, Li XJ, et al. The transcription factor SlHY5 regulates the ripening of tomato fruit at both the transcriptional and translational levels [J]. Hortic Res, 2021, 8(1): 83.

基因名称Gene name	上游引物Forward primer （5′-3′）	下游引物Reverse primer （5′-3′）
UBQ10	GATCCCACCAGACCAGCAA	ACCAAATGAAGGGTTGATTCCTT
FhGATA24	AACATTGTGGCGTTAGTG	GATTGACAGCCTTAGCAG
FhGATA22	GGCCTCAAGTTATCCATG	AGGTCCACTCCTCCAAAG
FhGATA26	TTCTCAAGCGAAAGTCAA	TGCTATGGAACTGGGATG
FhGATA18	TATCCAAGCCAAAGCAAT	CTCCAAAGCGGTGTAGAA
FhGATA12	GCGGAGACCATTTCATTG	GTTGGGATTAGCGTTCGT
FhGATA5	CATACCCACCAGCGAACT	GGATGACGAAGATGAGGC

基因名称Gene name	上游引物Forward primer （5′-3′）	下游引物Reverse primer （5′-3′）
UBQ10	GATCCCACCAGACCAGCAA	ACCAAATGAAGGGTTGATTCCTT
FhGATA24	AACATTGTGGCGTTAGTG	GATTGACAGCCTTAGCAG
FhGATA22	GGCCTCAAGTTATCCATG	AGGTCCACTCCTCCAAAG
FhGATA26	TTCTCAAGCGAAAGTCAA	TGCTATGGAACTGGGATG
FhGATA18	TATCCAAGCCAAAGCAAT	CTCCAAAGCGGTGTAGAA
FhGATA12	GCGGAGACCATTTCATTG	GTTGGGATTAGCGTTCGT
FhGATA5	CATACCCACCAGCGAACT	GGATGACGAAGATGAGGC

基因名称 Gene name	序列ID Sequence ID	氨基酸数 Number of amino acids （aa）	分子量Molecular weight （Da）	等电点 pI	不稳定指数 Instability index	脂肪族指数 Aliphatic index	亲水性 Hydropathicity	亚细胞定位预测 Subcellular localization prediction
FhGATA1	Fh1g02150.1	383	42 038.44	7.6	63.22	63.71	-0.564	细胞核Nucleus
FhGATA2	Fh1g02950.1	255	28 400.27	8.5	69.86	41.37	-0.832	细胞核Nucleus
FhGATA3	Fh1g12080.1	334	36 707.68	5.72	48.19	54.91	-0.618	细胞核Nucleus
FhGATA4	Fh1g20200.1	293	32 046.49	6.21	33.41	65.9	-0.787	细胞核Nucleus
FhGATA5	Fh1g24810.1	174	19 850.51	8.45	48.8	52.13	-0.718	细胞核Nucleus
FhGATA6	Fh1g24830.1	187	21 125.25	9.27	52.56	63.16	-0.663	细胞核Nucleus
FhGATA7	Fh2g01760.1	194	21 244.52	8.36	61.05	49.9	-0.962	细胞核Nucleus
FhGATA8	Fh2g01770.1	235	25 833.13	8.32	43.61	49.15	-1.065	细胞核Nucleus
FhGATA9	Fh3g10640.1	215	24 043.04	8.38	87.62	75.72	-0.713	细胞核Nucleus
FhGATA10	Fh3g25040.1	167	18 019.89	10.01	58.03	61.44	-0.513	细胞核Nucleus
FhGATA11	Fh3g27480.1	376	41 093.1	4.62	47.51	66.14	-0.798	细胞核Nucleus
FhGATA12	Fh3g27490.1	303	32 672.03	5.2	48.69	62.81	-0.637	细胞核Nucleus
FhGATA13	Fh4g04580.1	372	41 136.38	6.13	48.93	53.76	-0.817	细胞核Nucleus
FhGATA14	Fh4g05470.1	319	35 477.63	9.24	50.02	60.28	-0.829	细胞核Nucleus
FhGATA15	Fh4g05480.1	314	35 084.39	9.58	51.19	60.32	-0.8	细胞核Nucleus
FhGATA16	Fh4g10680.1	323	35 563.4	9.6	65.28	63.5	-0.733	细胞核Nucleus
FhGATA17	Fh5g07350.1	542	60 342.13	6.08	57.42	69.23	-0.656	细胞核Nucleus
FhGATA18	Fh5g35260.1	323	35 397.56	7.03	64.95	63.16	-0.733	细胞核Nucleus
FhGATA19	Fh5g42420.1	146	16 120.58	10.33	40.91	60.89	-0.696	细胞核Nucleus
FhGATA20	Fh5g48920.1	263	29 379.13	6.17	59.82	55.29	-0.89	细胞核Nucleus
FhGATA21	Fh7g06080.1	341	37 419.93	6.27	56.8	62.29	-0.61	细胞核Nucleus
FhGATA22	Fh8g08720.1	321	35 679.5	5.41	57.96	50.34	-0.681	细胞核Nucleus
FhGATA23	Fh8g12210.1	401	44 617.78	5.43	51.58	62.82	-0.694	细胞核Nucleus
FhGATA24	Fh8g25310.1	370	42 072.87	9.06	60.54	61.38	-0.663	细胞核Nucleus
FhGATA25	Fh9g01800.1	181	19 719.64	9.26	54.8	85.69	-0.387	细胞核Nucleus
FhGATA26	Fh9g11500.1	262	29 032.8	8.44	46.74	68.02	-0.599	细胞核Nucleus

基因名称 Gene name	序列ID Sequence ID	氨基酸数 Number of amino acids （aa）	分子量Molecular weight （Da）	等电点 pI	不稳定指数 Instability index	脂肪族指数 Aliphatic index	亲水性 Hydropathicity	亚细胞定位预测 Subcellular localization prediction
FhGATA1	Fh1g02150.1	383	42 038.44	7.6	63.22	63.71	-0.564	细胞核Nucleus
FhGATA2	Fh1g02950.1	255	28 400.27	8.5	69.86	41.37	-0.832	细胞核Nucleus
FhGATA3	Fh1g12080.1	334	36 707.68	5.72	48.19	54.91	-0.618	细胞核Nucleus
FhGATA4	Fh1g20200.1	293	32 046.49	6.21	33.41	65.9	-0.787	细胞核Nucleus
FhGATA5	Fh1g24810.1	174	19 850.51	8.45	48.8	52.13	-0.718	细胞核Nucleus
FhGATA6	Fh1g24830.1	187	21 125.25	9.27	52.56	63.16	-0.663	细胞核Nucleus
FhGATA7	Fh2g01760.1	194	21 244.52	8.36	61.05	49.9	-0.962	细胞核Nucleus
FhGATA8	Fh2g01770.1	235	25 833.13	8.32	43.61	49.15	-1.065	细胞核Nucleus
FhGATA9	Fh3g10640.1	215	24 043.04	8.38	87.62	75.72	-0.713	细胞核Nucleus
FhGATA10	Fh3g25040.1	167	18 019.89	10.01	58.03	61.44	-0.513	细胞核Nucleus
FhGATA11	Fh3g27480.1	376	41 093.1	4.62	47.51	66.14	-0.798	细胞核Nucleus
FhGATA12	Fh3g27490.1	303	32 672.03	5.2	48.69	62.81	-0.637	细胞核Nucleus
FhGATA13	Fh4g04580.1	372	41 136.38	6.13	48.93	53.76	-0.817	细胞核Nucleus
FhGATA14	Fh4g05470.1	319	35 477.63	9.24	50.02	60.28	-0.829	细胞核Nucleus
FhGATA15	Fh4g05480.1	314	35 084.39	9.58	51.19	60.32	-0.8	细胞核Nucleus
FhGATA16	Fh4g10680.1	323	35 563.4	9.6	65.28	63.5	-0.733	细胞核Nucleus
FhGATA17	Fh5g07350.1	542	60 342.13	6.08	57.42	69.23	-0.656	细胞核Nucleus
FhGATA18	Fh5g35260.1	323	35 397.56	7.03	64.95	63.16	-0.733	细胞核Nucleus
FhGATA19	Fh5g42420.1	146	16 120.58	10.33	40.91	60.89	-0.696	细胞核Nucleus
FhGATA20	Fh5g48920.1	263	29 379.13	6.17	59.82	55.29	-0.89	细胞核Nucleus
FhGATA21	Fh7g06080.1	341	37 419.93	6.27	56.8	62.29	-0.61	细胞核Nucleus
FhGATA22	Fh8g08720.1	321	35 679.5	5.41	57.96	50.34	-0.681	细胞核Nucleus
FhGATA23	Fh8g12210.1	401	44 617.78	5.43	51.58	62.82	-0.694	细胞核Nucleus
FhGATA24	Fh8g25310.1	370	42 072.87	9.06	60.54	61.38	-0.663	细胞核Nucleus
FhGATA25	Fh9g01800.1	181	19 719.64	9.26	54.8	85.69	-0.387	细胞核Nucleus
FhGATA26	Fh9g11500.1	262	29 032.8	8.44	46.74	68.02	-0.599	细胞核Nucleus

[1]	LI Zhi-qiang, LUO Zheng-qian, XU Lin-li, ZHOU Guo-hui, QU Si-yu, LIU En-liang, XU Dong-ting. Identification of the Soybean R2R3-MYB Gene Family Based on the T2T Genome and Their Expression Analysis under Drought and Salt Stress [J]. Biotechnology Bulletin, 2025, 41(5): 141-152.
[2]	HE Wei, LI Jun-yi, LI Xin-ni, MA Xue-hua, XING Yuan, CAO Xiao-ning, QIAO Zhi-ju, LIU Si-chen. Genome-wide Identification of U-box E3 Ubiquitin Ligase Gene Family in Setaria italica and Response Analysis to Abiotic Stress [J]. Biotechnology Bulletin, 2025, 41(5): 104-118.
[3]	LIU Xin, WANG Jia-wen, LI Jin-wei, MOU Ce, YANG Pan-pan, MING Jun, XU Lei-feng. Cloning and Expression Analysis of Three LdBBXs in Lilium davidii var. willmottiae [J]. Biotechnology Bulletin, 2025, 41(5): 186-196.
[4]	ZHAO Jing, GUO Qian, LI Rui-qi, LEI Ying-yang, YUE Ai-qin, ZHAO Jin-zhong, YIN Cong-cong, DU Wei-jun, NIU Jing-ping. Sequence Analysis and Induced Expression Analysis of GmGST Gene Cluster Genes in Soybean [J]. Biotechnology Bulletin, 2025, 41(5): 129-140.
[5]	WU Ya, YAO Run, YANG Han-ting, LIU Wei, YANG Shuai, SONG Chi, CHEN Shi-lin. Genome-wide Identification and Expression Analysis of SDR Gene Family in Mentha suaveolens ‘Variegata’ [J]. Biotechnology Bulletin, 2025, 41(5): 175-185.
[6]	YANG Chao-jie, ZHANG Lan, CHEN Hong, HUANG Juan, SHI Tao-xiong, ZHU Li-wei, CHEN Qing-fu, LI Hong-you, DENG Jiao. Functional Identification of the Transcription Factor Gene FtbHLH3 in Regulating Flavonoid Biosynthesis in Fagopyrum tataricum [J]. Biotechnology Bulletin, 2025, 41(4): 134-144.
[7]	SUN Tian-guo, YI Lan, QIN Xu-yang, QIAO Meng-xue, GU Xin-ying, HAN Yi, SHA Wei, ZHANG Mei-juan, MA Tian-yi. Genome-wide Identification of the DABB Gene Family in Brassica rapa ssp. pekinensis and Expression Analysis under Saline and Alkali Stress [J]. Biotechnology Bulletin, 2025, 41(4): 156-165.
[8]	WANG Tian-tian, CHANG Xue-rui, HUANG Wan-yang, HUANG Jia-xin, MIAO Ru-yi, LIANG Yan-ping, WANG Jing. Identification and Analysis of GASA Gene Family in Pepper （Capsicum annuum L.） [J]. Biotechnology Bulletin, 2025, 41(4): 166-175.
[9]	WANG Tian-xi, YANG Bing-song, PAN Rong-jun, GAI Wen-xian, LIANG Mei-xia. Identification of the Apple PLATZ Gene Family and Functional Study of the MdPLATZ9 Gene [J]. Biotechnology Bulletin, 2025, 41(4): 176-187.
[10]	SONG Jia-yi, SU Yun-li, ZHENG Xing-yan, XIA Wen-nian, YANG Dong-mei, HU Hui-zhen. Identification of the Snapdragon Expansin Gene Family and Screening of Its Genes Related to Resistance to Sclerotinia sclerotiorum [J]. Biotechnology Bulletin, 2025, 41(4): 227-242.
[11]	HUANG Jin-heng, HUANG Xi, ZHANG Jia-yan, ZHOU Xin-yu, LIAO Pei-ran, YANG Quan. Identification and Expression Analysis of the C3H Gene Family in Grona styracifolia across Different Varieties [J]. Biotechnology Bulletin, 2025, 41(4): 243-255.
[12]	BAN Qiu-yan, ZHAO Xin-yue, CHI Wen-jing, LI Jun-sheng, WANG Qiong, XIA Yao, LIANG Li-yun, HE Wei, LI Ye-yun, ZHAO Guang-shan. Cloning of Phytochrome Interaction Factor CsPIF3a and Its Response to Light and Temperature Stress in Camellia sinensis [J]. Biotechnology Bulletin, 2025, 41(4): 256-265.
[13]	LIU Tao, WANG Zhi-qi, WU Wen-bo, SHI Wen-ting, WANG Chao-nan, DU Chong, YANG Zhong-min. Identification and Expression Analysis of the GRAM Gene Family in Potato [J]. Biotechnology Bulletin, 2025, 41(4): 145-155.
[14]	ZHANG Yi-xuan, MA Yu, WANG Tong-tong, SHENG Su-ao, SONG Jia-feng, LYU Zhao-yan, ZHU Xiao-biao, HOU Hua-lan. Genome-wide Identification and Expression Profiles of DIR Gene Family in Potato [J]. Biotechnology Bulletin, 2025, 41(3): 123-136.
[15]	WANG Chen, LIU Guo-mei, CHEN Chang, ZHANG Jin-long, YAO Lin, SUN Xuan, DU Chun-fang. Genome-wide Identification and Expression Analysis of CCDs Family in Brassia rapa L. [J]. Biotechnology Bulletin, 2025, 41(3): 161-170.