Analysis of Verification Results by PCR Methods for Genetically Modified Double-resistant 12-5 Event-specific Maize

doi:10.13560/j.cnki.biotech.bull.1985.2020-0299

Abstract

Abstract: Insect-resistant and herbicide-tolerant genetically modified maize DR12-5 is a new transgenic strain independently researched and developed in China. It has been approved for the agricultural GMO safety certificate on January 21,2020,and thus has broad application prospects. The event-specific PCR method is one of the most effective detection methods in safety supervision for transgenic organisms,which can identify the genetically modified products. In this study,we organized 8 laboratories to verify the event-specific qualitative and quantitative PCR methods of the double-resistant（DR）12-5 transformants provided by its research and development institutions. The verified results showed that both the qualitative and quantitative PCR detection methods had good stability,strong specificity and high sensitivity. The quantitative PCR method can accurately and quantitatively detect DR 12-5 samples in mass fractions of 5% and 0.5%,and has good repeatability and reproducibility,which conforms the requirements of relevant standards. This study is conducive to the establishment and improvement of subsequent standard methods and provides technical support and decision-making basis for the safety supervision of GMOs in China.

Key words: genetically modified maize, qualitative PCR, quantitative PCR, methods validation

WANG Hao-qian, XIAO Fang, YANG Lei, MIAO Qing-mei, ZHANG Xu-dong, ZHANG Xiu-jie. Analysis of Verification Results by PCR Methods for Genetically Modified Double-resistant 12-5 Event-specific Maize[J]. Biotechnology Bulletin, 2020, 36(5): 48-55.

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