A Modified Method for Quantification of L-lysine

Biotechnology Bulletin ›› 2013, Vol. 0 ›› Issue (2): 190-194.

• Research report • Previous Articles Next Articles

A Modified Method for Quantification of L-lysine

Jiang Anna^1，2 Cao Mingfeng^2，3 Li Qinggang^2，3 Zheng Ping^2，3 Yang Hongjiang¹ Sun Jibin^2，3

（1. School of Biological Engineering，Tianjin University of Science and Technology，Tianjin 300457 ；2. Tianjin Institute of Industrial

Received:2012-07-05 Revised:2013-02-27 Online:2013-02-26 Published:2013-02-27
Contact: 孙际宾，男，研究员，博士生导师，研究方向：微生物代谢工程；E-mail ：sun_jb@tib.cas.cn

Abstract

Abstract: A modified method was established to determine lysine concentration using ninhydrin-ferric reagents. Unlike the traditional method which measures the total amino acids, our modification showed high specificity and favorable linearity to lysine（0-200 mmol/L）. In our protocol the characteristic absorption at 480 nm was measured after the reaction mixture was boiled for 35 minutes in disodium hydrogen phosphate-citrate buffer at pH2.2. The method not only successfully excludes the interference of impurities including proline, ornithine, glycine, arginine and histidine, but offers the accurate and reliable determination of the lysine in the fermentation broth directly.

Key words: Lysine, Ninhydrin-ferric reagents, Color reaction, Specific detection

Jiang Anna, Cao Mingfeng, Li Qinggang, Zheng Ping, Yang Hongjiang, Sun Jibin. A Modified Method for Quantification of L-lysine[J]. Biotechnology Bulletin, 2013, 0(2): 190-194.

References

［1] Zelder O, Klopprogge C, Sch?ner H, et al. L-lysine fermentation［J]. Microbial Technolol, 2005, 1(1)：211-240.
[2] Rao D, Razak SA, Praveena B, Swamy A. Dissolved oxygen concentration analysis of L-Lysine fermentation production by Corynebacterium glutamicum［J]. The Internet Journal of Pharmacology, 2008, 6(1), DOI:10.5580/19f0.
[3] Anastassiadis S. L-Lysine fermentation［J]. Microbial Technolol, 2007, 1(1)：11-24.
[4] Nelofer R, Syed Q, Nadeem M. Statistical optimization of process variables for L-lysine production by Corynebacterium glutamicum［J]. Türk Biyokimya Dergisi, 2008, 33(2)：50-57.
[5] Debatosh D, Akshay B, Vidya C. Lysine:Is it worth more?［J]. Cytotechnology, 2001, 36:3-32.
[6] Oh JW, Kim SJ, Cho YJ, et al. Strain of corynebacterium glutamicum and method for producing L-lysine:US, 5268293［P]. 1993-12-7.
[7] Nakazawa M, Takahashi D, Onishi N, et al. L-Lysine fermentation: US, 20067012152［P]. 2006.
[8] Nakazawa M, Takahashi D, Onishi N, et al. Method for producing lysine derivative:US, 20067012152［P]. 2006.
[9] Emmert JL, Douglas MW, Boling SD, et al. Bioavailability of lysine from a liquid lysine source in chick［J]. Poultry Science, 1999, 78 (3): 383-386.
[10] 周伟. 浅谈赖氨酸行业现状和发展趋势［J]. 发酵科技通讯, 2007(6)：31-35.
[11] 闫洪颖, 柳晓峰. 2010 年中国赖氨酸市场回顾及2011 年展望［J]. 中国畜牧杂志, 2011, 47(4)：23-30.
[12] 王镜岩, 朱圣庚, 徐长法. 生物化学［M]. 北京：高等教育出版社, 2002:138-139.
[13] Friedman M. Applications of the ninhydrin reaction for analysis of amino acids, peptides, and proteins to agricultural and biomedical sciences［J]. Agric Food Chem, 2004, 52:385-406.
[14] Dietzen DJ, Weindel AL, Carayannopoulos MO, et al. Rapid comprehensive amino acid analysis by liquid chromatography/ tandem mass spectrometry:comparison to cation exchange with post-column ninhydrin detection［J]. Rapid Commun Mass Spectrom, 2008, 22(22)：3481-3488.
[15] Chinard FP. Photometric estimation of proline and ornithine［J]. J Biol Chem, 1952, 91(5)：91-95.
[16] Hsieh CL, Hsiung KP, Su JC. Determination of lysine with ninhydrin-ferric reagent［J]. Analytical Biochemistry, 1995, 224 (1)：187-189.
[17] 杨菁, 孙黎光, 白秀珍, 周海涛. 异硫氰酸苯酯柱前衍生化反相高效液相色谱法同时测定18 种氨基酸［J]. 色谱, 2002, 20(4)：369-371.
[18] Beckwith AC, Paulis JW, Wall JS. Direct estimation of lysine in corn meals by the ninhydrin color reaction［J]. Food Chem, 1975, 23(2)：194-196.
[19] 刘飞飞, 李群, 于岚. 茚三酮比色法定量检测赖氨酸条件的研究［J]. 中国食品添加剂, 2010, 5:223-234.

[1]	SHI Cheng-long, WANG Xi-wu, LI An-qi, QIAN Sen-he, WANG Zhou, ZHAO Shi-guang, LIU Yan, XUE Zheng-lian. Effect of ε-Polylysine on the Cell Structure and Biofilm Formation of Cronobacter sakazakii [J]. Biotechnology Bulletin, 2022, 38(9): 147-157.
[2]	TANG Qian-qian, LIN Chu-yu, TAO Zeng. Research Progress in Histone Demethylase in Plant [J]. Biotechnology Bulletin, 2022, 38(7): 13-22.
[3]	FENG Cui-lian, WAN Yue, WANG Jun-gang, FENG Xiao-yan, ZHAO Ting-ting, WANG Wen-zhi, SHEN Lin-bo, ZHANG Shu-zhen. Establishment of a Transformant-specific Detection Method for Cry1Ac-2A-gna Transgenic Sugarcane BCG-17 [J]. Biotechnology Bulletin, 2021, 37(5): 248-258.
[4]	ZHENG Lu, SHEN Ren-fang, LAN Ping. Research Progress of Plant Lysine Acetylproteome Modified in Non-histone Protein [J]. Biotechnology Bulletin, 2021, 37(1): 77-89.
[5]	ZHOU Wei, WáNG Yu, XIE Li-nán. ádvánces in the Estáblishment ánd Inheritánce of Plánt Histone Lysine Methylátion [J]. Biotechnology Bulletin, 2020, 36(4): 117-130.
[6]	JIN Yong-mei, MA Rui, YU Zhi-jing, LIN Xiu-feng. Integration Analysis of Exogenous Gene and Line-Specific Detection in the Insect-Resistant cry1C-Transgenic Rice Jishengjing3 [J]. Biotechnology Bulletin, 2019, 35(3): 6-12.
[7]	XU De-yu, ZHENG Xiao-mei, ZHAO Jing, ZHENG Ping, ZHAO Shu-xin. Aspartokinase G359D from Corynebacterium glutamicum Relieves the Synergistic Inhibition of Lysine and Threonine [J]. Biotechnology Bulletin, 2017, 33(11): 143-152.
[8]	Lü Binna, Liang Wenxing. The Research Progress of Protein Acetylation [J]. Biotechnology Bulletin, 2015, 31(4): 166-174.
[9]	Li Xiaofei,Tan Xiaoli,Li Jun,Wu Yuhua,Cao Yinglong. Construction and Application of a Plasmid Reference Molecule for Detecting Transgenic Rice [J]. Biotechnology Bulletin, 2013, 0(7): 71-77.

A Modified Method for Quantification of L-lysine

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 9

Recommended Articles

Metrics

Comments