Biotechnology Bulletin ›› 2013, Vol. 0 ›› Issue (2): 195-200.

• Research report • Previous Articles    

Investigation of Microbial Species in Domestic Refrigerator

Yang Songzhen1，2 Feng Guangda1 Yao Qing2 Wang Yonghong1 Yao Yuxin3 Zhu Honghui1   

  1. （1. Guangdong Institute of Microbiology，State Key Laboratory of Applied Microbiology（Ministry—Guangdong Province Jointly Breeding Base），South China，Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application，Guangdong Open Laboratory of Applied Microbiology，Guangzhou 510070 ；2. College of Horticulture，South China Agricultural University，Guangzhou 510642 ；3.Primary School of Xianlie Middle Road，Guangzhou 510070）
  • Received:2012-08-16 Revised:2013-02-27 Online:2013-02-26 Published:2013-02-27

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Abstract: It was to understand microbial species and their pathogenic risk in domestic refrigerator. Five domestic refrigerators were randomly selected, from different positions of which bacteria and fungi were isolated using media of nutrition agar（NA）, potato dextrose agar （PDA）and Gause’s No.1. The isolates were identified by 16S rRNA gene, 26S rRNA gene or ITS sequences analysis and morphological characteristics observation using the scanning electron microscopy（SEM）. Total of 84 bacterial and 74 fungal strains were isolated. Most of bacteria belonged to the genera of Bacillus, Staphylococcus, Kocuria and Pseudomonas. Fungal strains mainly belonged to the genera of Cladosporium, Aspergillus and Penicillium. Pathogenic microbes in domestic refrigerators were mainly composed of the strains in Staphylococcus, while others distributed randomly. Variety of bacteria and fungi existed in domestic refrigerators. Domestic refrigerators were mainly contaminated by Staphylococcus. The safe problem of domestic refrigerator should be taken seriously enough.

Key words: Domestic refrigerator, Bacteria Fungi, Staphylococcus, Pathogenic