Biotechnology Bulletin ›› 2013, Vol. 0 ›› Issue (3): 102-106.

• Research report • Previous Articles     Next Articles

Molecular Identification of Hybrid Clones Between Populus deltoides Bartr. and P. ussuriensis Kom.

Jiang Xibing1, 2, Zhang Zhiyi2, Gong Bangchu1   

  1. 1. Research Institute of Subtropical Forestry,CAF,Fuyang 311400 ；2. National Engineering Laboratory for Tree Breeding,NDRC ；Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants,MOE,Beijing Forestry University,Beijing 100083）
  • Received:2012-06-26 Revised:2013-03-21 Online:2013-03-20 Published:2013-03-21

PDF (PC)

583

Abstract: Molecular identification of 12 hybrid clones were carried out using primer combinations of EcoR I + 2 / Mse I + 3 by adopting AFLP marker technology. The results were as followeds: 10 primer combinations with more amplification bands were selected from 32 primer combinations, the total number of bands were between 7 and 52, number of polymorphism bands were from 2 to 28, polymorphism level of those primer combinations were from 23.5% to 41.6%, and lower level of polymorphism bands and proportion showed that the relationships of the samples were closed. The results of UPGMA cluster analysis and pedigree relations of 12 clones were the same, which indicated that clones with the same parents had higher genetic similarity coefficient. The fingerprinting map of 12 hybrid clones were draw using 9 polymorphism bands from 6 primer combinations, which would provide bases of identification and protection for hybrid clones.

Key words: Poplar, Hybrid, AFLP, Molecular identification, Fingerprinting map