Effects of Co-culture with Helper Bacteria on the Secondary Metabolites of Myxococcus fulvus

Abstract

Abstract: The research of secondary metabolites of myxobacteria was restricted due to it's low content under the routine laboratory culture conditions. This study discussed the effect of two helper bacteria to Myxococcus fulvus producing secondary metabolites in liquid fermentation culture conditions. The results showed that the yield and diversity of secondary metabolites were improved by adding the two helper bacteria. After the co-culture of M. fulvus with 42 or 43 helper bacterium, the yield of the secondary metabolites increased from 0.05 g/L to 0.40 g/L and from 0 g/L to 0.45 g/L, respectively. The maximum yield was obtained for 42 (0.64 g/L) and 43 (0.69 g/L) helper bacterium, when the former was inoculated between 72 to 84 h or the later was cultured with M. fulvus simultaneously. However, the effect of the two helper bacteria on diversity of the secondary metabolites was different. The original secondary metabolites component of M. fulvus was changed by adding 43 helper bacteria but not 42. In conclusion, co-culture with helper bacteria can improve the secondary metabolism of M. fulvus, which would help analyze the secondary metabolites of myxobacteria.

Key words: Myxococcus fulvus, Helper bacteria, Co-culture, Secondary metabolites

Huang Yan, Hu Jianwei, Zhu Honghui, . Effects of Co-culture with Helper Bacteria on the Secondary Metabolites of Myxococcus fulvus[J]. Biotechnology Bulletin, 2013, 0(5): 184-189.

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