Construction of Recombinant E. coli JM109 Capable of Producing 3-Hydroxypropionic Acid and Its Fermentation Condition Optimization

Abstract

Abstract: The expression vector pEtac harboring aldh gene and dhaB gene was transformed into E.coli JM109 to construct recombinant E.coli JM109(pEtac-dhaB-aldh). And the response surface method(RSM)was firstly applied to determine and optimize the fermentation condition for the novel recombinant E.coli JM109(pEtac-dhaB-aldh)capable of producing 3- hydroxypropionic acid. A mathematical model was then developed to show the effect of each medium composition and their interactions on the production of 3- hydroxypropionic acid. The model estimated that, a maximal yield of 3-hydroxypropionic acid(5.4 g/L)could be obtained when the concentrations of glycerol, VB₁₂, yeast extract KH₂PO₄ were set at 62 g/L, 0.05 g/L, 6.2 g/L and 7.4 g/L, respectively. These predicted values were also verified by validation experiments. Compared with the values obtained by other runs in the experimental design.The yield and productivity under the optimal parameters and process can reach 5.8 g/L.

Key words: Recombinant, E. coli, Construction 3- hydroxypropionic acid, Response surface methodology, Optimization

Zhang Xiaomei, Li Xinsheng, Xu Zhenghong. Construction of Recombinant E. coli JM109 Capable of Producing 3-Hydroxypropionic Acid and Its Fermentation Condition Optimization[J]. Biotechnology Bulletin, 2013, 0(6): 200-208.

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