Genetic Diversity of Different Geographical Populations of Simulium quinquestriatum Based on ISSR Analysis

Abstract

Abstract: In this paper, the genetic diversity of geographical populations of S. quinquestriatum from China was investigated with ISSR method using eight selective primers in order to clarify thegenetic diversity of eight different geographic populations of S. quinquestriatum. Establishment of ISSR fingerprint of S. quinquestriatum from eight populations of 160 individuals in this study. The results showed that total 111 bands were generated, of which 96 bands were polymorphic bands（the percentage of polymorphic band, P= 86.47％）. The characteristic ISSR pattern for each individual was detected. S. quinquestriatum displayed the higher degree of genetic diversity at the species level（P=86.4%, A=1.991 0±0.094 9, A _E=1.553 2±0.334 5, H=0.323 1±0.157 9, I=0.484 7±0.203 7）than the population level（P=78.37%, A=1.492 1±0.499 7, A _E=1.316 6±0.370 0, H=0.180 1±0.206 6, I=0.265 5±0.295 0）. Results of AMOVA analysis demonstrated that the among-population component had a high degree which accounted for 59% of the total genetic differentiation；while the within-population component accounted for 41%. The gene flow that among-population is 0.347 5. It is indicated that the main factors for the high degree of genetic differentiation among different geographic populations of S. quinquestriatum are geographic barrier（mountains and plains）and habitat fragmentation.

Key words: Simulium quinquestriatum, Populations ISSR, Genetic diversity, Genetic differentiation

Xiu Jiangfan, Zhang Chunlin, Chen Hanbin. Genetic Diversity of Different Geographical Populations of Simulium quinquestriatum Based on ISSR Analysis[J]. Biotechnology Bulletin, 2013, 0(7): 107-113.

References

[1] Adler PH, Crosskey RW. World Blackflies（Diptera：Simuliidae）：A Comprehensive Revision of the Taxonomic and Geographical Inventory [2010]. http：//entweb.clemson.edu/biomia/pdfs/blackflyinventory.pdf
[2] 陈汉彬, 安继尧.中国黑蝇（双翅目：蚋科）[M].北京：科学出版社, 2003：258-259.
[3] Fukuda M, Takaoka H, Uni S, et al. Infective Larve of five Onchocerca species from experimentally infected Simulium species in an area of Zoontic Onchocerciasis in Janpan [J]. Parasite, 2008, 15（2）：111-119.
[4] Zietkiewicz E, Rafslski A, Labuda D. Genome fingerprinting by simple sequence repeat（SSR）anchored polymerasechain re-action amplification [J]. Genomics, 1994, 20：176-183.
[5] 周延清. DNA分子标记技术在植物研究中的应用[M]. 北京：化工工业出版社, 2005：68-70.
[6] Agostini G, Echeverrigaray S, Souza-Chies TT. Geneticrela-tionships among South American species of Cunila D. Royen ex L. based on ISSR [J]. Plant Syst Evol, 2008, 274：135-141.
[7] Dje Y, Tahi CG, Bi AI, et al. Use of ISSR markers to assess genetic diversityof African edibleseeded Citrullus lanatus landraces [J]. Scientia Horticulturae, 2010, 124（2）：159-164.
[8] Du XY, Zhang QL, Luo ZR. Development of retrotransposon primers and their utilization for germplasm identification in Diospyros spp.（Ebenaceae）[J]. Tree Genetics & Genomes, 2009, 5：235-245.
[9] Gupta S, Pandey-Rai S, Srivastava S, et al. Construction of genetic linkage map of the medicinal and ornamental plant Catharanthus roseus [J]. Journal of Genetics, 2007, 86（3）：259-268.
[10] Dusinsky R, Kudela M, Stloukalova V, et al. Use of inter-simple sequence respeat（ISSR）markers for discrimination between and within species of blackflies（Diptera：Simuliidae）[J]. Section Cellular and Molecular Biology Biologia, 2006, 61（3）：299-304.
[11] 廉国盛.山西蚋类区系研究和中国8亚属23蚋种ISSR分子进化研究[D].贵阳：贵阳医学院，2009.
[12] 范海荣, 夏永静.四种全血基因组DNA提取方法的比较[J].中国动脉硬化杂志, 2002, 10（6）：535-536.
[13] Yeh FC, Yang RC, Boyle T, et al. POPGENE, the user friendly shareware for population genetic analysis [M]. Edmonton：University of Alberta, 1997.
[14] Nei M, Li WH. Mathematical model for studying genetic variation in terms of restriction endonucleases [J]. Proc Natl Acad Sci USA, 1979, 76（10）：5269-5273.
[15] Kumar S, Tamura K, Jakobsen IB, et al. MEGA2：Molecular evoutionary genetics analysis software [J]. Bioinformatics, 2001, 17（12）：1244-1245.
[16] 陈俊秋, 慈秀芹.樟科濒危植物思茅木姜子遗传多样性的ISSR分析[J].生物多样性, 2006, 14（5）：410-420.
[17] 王晓明, 赖燕玲.深圳梅林仙湖苏铁野生种群遗传多样性ISSR分析[J].中山大学学报：自然科学版, 2006, 45（3）：82-85.
[18] 朱勋, 杨家强.小菜蛾不同地理种群遗传多样性的ISSR标记研究[J].昆虫学报, 2012, 55（8）：981-987.
[19] 陈灵芝.中国的生物多样性—现状及其保护对策[M].北京：科学出版社, 1993：11-15.
[20] 王定江, 杨汉远, 钟扬, 任竹梅.贵州省八个种群角倍蚜ISSR遗传多样性[J].生态学杂志, 2008, 27（10）：1729-1733.
[21] 张颖, 李菁, 王振营.中国桃蛀螟不同地理种群的遗传多样性, 昆虫学报, 2010, 53（9）：1022-1029.
[22] Wright S. Evolution and the genetics of populations.//Variability within and among natural populations [M]. Chicago：University of Chicago Press, 1978.
[23] Slatkin M. Gene flow in natural populations [J]. Annual Review of Ecology & Systematics, 1985（16）：393-430.
[24] Peter H, Adler A. Evolution, epidemiology, and population genetics of black flies（Diptera：Simuliidae）[J]. Genetics and Evolution, 2010（10）：846-865.