Biotechnology Bulletin ›› 2016, Vol. 32 ›› Issue (4): 159-167.doi: 10.13560/j.cnki.biotech.bull.1985.2016.04.021

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Genetic Diversity Analysis of Populus yunnanensis by SRAP Markers

YAN Lu-xi1，2, LI Jia-man1，2, YUAN Tao1，2 ,ZHOU An-pei1，2 ,ZONG Dan1，2, LI Dan3, XIN Pei-yao1，2，4 ,HE Cheng-zhong1，2，4   

  1. 1. Key Laboratory for Forest Genetic and Tree Improvement & Propagation in Universities of Yunnan Province，Southwest Forestry University，Kunming 650224;
    2. Key Laboratory of Biodiversity Conservation in Southwest China，State Forestry Administration，Southwest Forestry University，Kunming 650224;
    3. Yunnan Academy of Biodiversity，Southwest Forestry University，Kunming 650224;
    4. Key Laboratory for Forest Resources Conservation and Use in the Southwest Mountains of China，Ministry of Education，Southwest Forestry University， Kunming 650224
  • Received:2015-04-22 Online:2016-04-25 Published:2016-04-26

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Abstract: To figure out the genetic diversity and genetic structure of Populus yunnanensis，208 samples from 7 populations were analyzed by sequence-related amplified polymorphism（SRAP）markers. With selected 7 pairs of primers，146 bands were obtained，in which 73 fragments（50%）were polymorphic. The values at the species level were 1.500 0 for observed alleles number（Na），1.230 9 for effective alleles number（Ne），0.136 6 for Nei’s genetic diversity index（H），and 0.210 0 for Shannon’s information index（I）. The genetic differentiation coefficient（Gst）was 0.529 4 and gene flow（Nm）was 0.444 4 and on intermediate stage，indicating that genetic variability of P. yunnanensis mainly took place among populations. Similarly，AMOVA showed that the genetic diversity among populations accounted for 55.61% of total. Clustering analysis by UPGMA，PCoA and Bayesian constantly revealed that population Lijiang and Qujing were in close relationship，and the same for population Chuxiong and Zhaotong. In addition，no correlation between the genetic distance and geographical distance was found by Mantel Test.

Key words: Populus yunnanensis, genetic diversity, genetic structure, SRAP marker