Screening and Identification of a High-yield Glucoamylase Bacteria

Abstract

Abstract:

An high-yield glucoamylase bacterial strain of ZWL-1（GenBank accession number of the 18S rDNA gene：KC433410）used dilution plate method was isolated from a soy processing plant in Jilin sewage outfall at soil samples . The species identification was made on it, on the basis of morphological observation and through 18S rDNA sequence analysis further to the bacterial strain. The 18S rDNA sequence shows that the homology of this bacterial strain and the18S rDNA of Aspergillus niger was above 99.0%. As shown in the phylogenetic tree, strain ZWL-1 and Aspergillus sp. LQ21 constituted a branch and they constituted a high-order branch together with Aspergillus niger strain.CS1-1. Thus, the primary identification proved that this bacterial strain is Aspergillus niger.

Key words: 18S rDNA Screening Identification, Aspergillus niger, Glucoamylase

Zhang Wenli,Yu Hansong,Piao Chunhong,Wang Shuting,Hu Yaohui. Screening and Identification of a High-yield Glucoamylase Bacteria[J]. Biotechnology Bulletin, 2013, 0(7): 131-135.

References

[1] 王凤寰, 孟青青.嗜热糖化酶基因在重组黑曲霉工程菌中的表达及酶学性质初步研究[J].生物技术通报, 2012（7）：119-125.
[2] Dohmen RJ. Cloning of the Schwanniomuces occidentalis glucoamyl-ase gene GAM1 and its expression in Saccharomyces cerevisiae [J].Gene, 1990, 95（1）：111-121.
[3] Neffs JM, Van De Peer Y, Hendiuks L, et al. Compilation of small ribosomal subunit RNA sequences [J]. Nucleic Acids Res, 1990, 18（Suppl.）：2237-2317.
[4] 李祖明, 张洪勋, 薛文通.一株高产碱性内切聚半乳糖醛酸酶生产菌的筛选与鉴定[J].生物技术通报, 2010（2）：178-183.
[5] 孙俊良, 李新华, 梁新红.不同碳源对黑曲霉产糖化酶活力的影响[J].食品科学, 2008（8）：433-436.
[6] 中华人民共和国国家质量监督检验检疫总局, 中国国家标准化管理委员会.GB8267—2006食品添加剂糖化酶制剂[S].北京：中国标准出版社, 2006.
[7] 何毅婷, 杨汝德.以18S rDNA 序列鉴定一株产纤维素酶真菌[J].现代食品科技, 2008（7）：638-640.
[8] 奥斯伯FM, 布伦特R, 等.精编分子生物学实验指南[M].第5版.北京：科学出版社, 2008.
[9] 胡耀辉, 徐云, 于寒松, 等.北豆腐中主要腐败微生物的分离与鉴定[J].食品工程, 2010（9）：108-110.
[10] Thompson JD, Gibson TJ, Plewniak F, et al. The CLUSTAL X windows interface：flexible strategies for multiple sequence alignment aided by quality analysis tools [J]. Nucleic Acids Res, 1997, 25（24）：4876-4882.
[11] Kumar S, Tamura K, Jakobsen IB, et al. MEGA2：molecular evolutionary genetics analysis software [J]. Bioinformatics, 2001, 17（12）：1244-1245.
[12] 刘洋, 赵婷, 姚粟.一株芝麻香型白酒高温大曲嗜热放线菌的分离与鉴定[J].生物技术通报, 2012（10）：210-216.
[13] 王瑞礼.医学真菌学-实验室检验指南[M].北京：人民卫生出版社, 2005：0-69.
[14] Saitou M, Nei M. The Neighbour-joining method：a new method for reconstructing phylogenetic trees [J]. Mol Biol Evol, 1987（4）：406-425.
[15] Felsentein J. Confidence limits on phylogenies：an approach using the Bootstrap [J]. Evolution, 1985, 39（4）：783-791.