Abstract:

Bioinformatic method was used for the comparative analysis of CBF1 gene sequences in cold acclimation, conservative domains among the sequences were determined for degenerate primer designing, the defined PCR conditions and Reverse PCR clone were adopted to clone the full length CBF1 genes from genes of Vitis amurensis which is in cold acclimation. Sequence analysis showed that the ORF lengths of CBF1 gene is 753 bp and has no intron in coding domain, and encoded proteins of 251 residues. Furthermore, their molecular weights and isoelectric points of 27.503 kD and 3.11 were predicted from the calculated pI values. Similarity of amino acids to many plant which is Hordeum vulquare and Oryza sativa is 80.7%, it have a highly conserved sequence in AP2/EREBP, this demonstrate we clone transcription factor CBF1 genes of Vitis amurensis . It is named ViCBF1 which is registered in GenBank, number is DQ517296.

Key words: Wild grape(Vitis amurensis), Cold acclimation, Transcription factor, CBF1 gene, COR gene, Cloning