Transcriptome Library Construction and Sequencing from Chemically Induced Aquilaria sinensis

Abstract

Abstract: Modified guanidinium isothiocyanate-CTAB method was used to isolate the total RNA from five-year-old Aquilaria sinensis treated by chemical induction one year ago. The mRNA of A. sinensis was enriched from the total RNA and broken into short fragments, and then the cDNA library was established for RNA-Seq. As a result, 54 685 634 clean reads was obtained after sequencing with a total length of 4 921 707 060 nt. The value of Q20 was up to 97.45%, exhibiting good sequencing quality. After the initial assembly of sequence data, these clean reads were assembled to 190 109 contigs, which were then assembled to 83 467 unigenes with a total length of 58 569 625 nt and an average length of 702 nt after the further assembly. The value of N50 was up to 1 120. There were 1 691 unigenes longer than 3 000 nt, accounted for 2.03% of all unigenes. The good quality of assembly showed the information on the transcriptome of A. sinensis was well preserved, which laid the foundation for digital gene expression analysis associated with agarwood formation of A. sinensis.

Key words: Aquilaria sinensis, Chemical induction, Transcriptome library, Sequencing, Assembly, Unigenes

Wu Hongqing, Wang Lei, Tao Meihua, Gao Xiaoxia, Bai Ling, Zhang Weimin. Transcriptome Library Construction and Sequencing from Chemically Induced Aquilaria sinensis[J]. Biotechnology Bulletin, 2013, 0(8): 63-67.

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