鳜不同孵化时期miRNA转录组分析及生长相关miRNA鉴定

doi:10.13560/j.cnki.biotech.bull.1985.2018-0261

生物技术通报 ›› 2018, Vol. 34 ›› Issue (8): 181-189.doi: 10.13560/j.cnki.biotech.bull.1985.2018-0261

鳜不同孵化时期miRNA转录组分析及生长相关miRNA鉴定

赵岩, 曹晓颖, 周昊天, 宋凌元, 涂翰卿, 黄思颖, 赵金良

1. 上海海洋大学农业部淡水产种质资源重点实验室,上海 201306;
2. 上海海洋大学水产动物遗传育种中心上海市协同创新中心,上海 201306;
3. 上海海洋大学水产科学国家级实验教示范中心,上海 201306

收稿日期:2018-03-19 出版日期:2018-08-26 发布日期:2018-09-04
作者简介:赵岩,男,博士,研究方向：水产动物遗传与育种;E-mail：y_zhao@shou.edu.cn
基金资助:
上海市自然科学基金项目（16ZR1415300）,国家现代农业产业技术体系专项（CARS-46）,国家自然科学基金项目（31602128）

Analysis of miRNA Transcriptome in Early Developmental Stage and Identification of Growth-related miRNA of Siniperca chuatsi

ZHAO Yan, CAO Xiao-ying, ZHOU Hao-tian, SONG Ling-yuan, TU Han-qing, HUANG Si-ying, ZHAO Jin-liang

1. Key Laboratory of Freshwater Aquatic Genetic Resources,Ministry of Agriculture,Shanghai Ocean University,Shanghai 201306;
2. Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding,Shanghai Ocean University,Shanghai 201306;
3. National Demonstration Center for Experimental Fisheries Science Education,Shanghai Ocean University,Shanghai 201306

Received:2018-03-19 Published:2018-08-26 Online:2018-09-04

摘要/Abstract

摘要： MicroRNA是一类20-24 nt核苷酸长度的参与基因转录后水平调控的非编码小分子RNA。利用高通量测序技术获得鳜（Siniperca chuatsi）孵化后第3天、第17天和第28天全鱼miRNA转录组,共鉴定出1 084个miRNAs,其中432个已知、624个保守、28个新发现。第17天时鉴定出的miRNA个数最多,为926个。在3个发育时期都表达的有628个miRNAs,只在各时期中特异表达的分别有71、104和70个miRNAs。一些与鳜发育相关的miRNAs在上述3个时期呈现持续上调或下调的表达特点。进一步的实验表明,miR-199-5p和miR-203可能与鳜生长发育相关,相应的靶基因分别是WnT 和 MyoD。

关键词: 鳜, 高通量测序, microRNA, 发育, 生长

Abstract: MicroRNA（miRNA）is a kind of non-coding small RNA molecules with the length of about 20-22 nt,and they are involved in regulating the expression of target genes at the post-transcriptional level. High-throughput sequencing technology was employed to analyze the miRNA transcriptome of the whole larvae of Siniperca chuatsi at day 3,day 17 and day 28 after hatching. A total of 1084 miRNAs were identified,including 432 known,624 conserved,and 28 new miRNAs. miRNAs of the 17-day larvae were identified the most with 926. The 628 miRNAs were ubiquitously expressed at all the days sampled,while 71,104 and 70 miRNAs were specially expressed at day 3,day 17 and day 28,respectively. Several miRNAs that were involved in development showed sustained up or down regulation patterns. Further results suggested that miR-199-5p/miR-203 were correlated with the growth and development of mandarin fish,and the corresponding target genes were WnT and MyoD,respectively.

Key words: mandarin fish, high-throughput sequencing, microRNA, development, growth

赵岩, 曹晓颖, 周昊天, 宋凌元, 涂翰卿, 黄思颖, 赵金良. 鳜不同孵化时期miRNA转录组分析及生长相关miRNA鉴定[J]. 生物技术通报, 2018, 34(8): 181-189.

ZHAO Yan, CAO Xiao-ying, ZHOU Hao-tian, SONG Ling-yuan, TU Han-qing, HUANG Si-ying, ZHAO Jin-liang. Analysis of miRNA Transcriptome in Early Developmental Stage and Identification of Growth-related miRNA of Siniperca chuatsi[J]. Biotechnology Bulletin, 2018, 34(8): 181-189.

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鳜不同孵化时期miRNA转录组分析及生长相关miRNA鉴定

Analysis of miRNA Transcriptome in Early Developmental Stage and Identification of Growth-related miRNA of Siniperca chuatsi

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