关键词: 生长期, 麻山药, 微生物多样性, 群落结构, 高通量测序

Abstract: This work is to study the dynamic change law of microbial diversity of soil samples at different reproductive periods from yam rhizosphere. By adopting the method of high-throughput sequencing technology,we studied the soil microbial community structure changes of yam root zone in seedling,flowering and harvest stage. Results showed that the diversity and abundance of bacteria in different growth period did not have significant variation,while there was more apparent variation for fungi,and the variation increased gradually from the start growing season to harvest,reached the highest degree of diversity index and abundance in harvest stage. Proteobacteria and Acidobacteria were the dominant bacteria at phylum level,which had over 50% proportion in different periods. Actinomucor and Mortierella were the dominant fungi at genus level,which accounted for 15% proportion in different periods. Combined with the obvious changes of other advantage bacterial group in different growth stages,it can be seen that these flora are closely related to the growth and development of yam.

Key words: development phase, yam, microbial diversity, community structure, high-throughput sequencing