miR-124 Regulates Porcine Adipocyte Differentiation Trough Targeting TNF-α

doi:10.13560/j.cnki.biotech.bull.1985.2017.03.025

Abstract

Abstract: In the previous research,porcine TNF-α was found to be the target of miR-124 by dual luciferase assay. Here we verify whether miR-124 has the effect on the differentiation of porcine adipocyte. Porcine pre-adipocyte was transfected with miR-124 mimics or inhibitor to over-express or to repress miR-124,and then induced into mature adipocytes. The accumulation of lipid droplets was observed using Oil Red O staining and the amount of glycerol and triglycerides（TG）were detected by TG assay and glycerol assay. The expressions of PPARγ（proliferator-activated receptor-γ）,FASN（fatty acid synthase）,and HSL（hormone-sensitive lipase）were detected by real time fluorescence quantitative PCR. The result showed that overexpression of miR-124 repressed the expression of TNF-α protein,therefore,lipid droplets were more than that in the control,TG increased significantly（P < 0.01）,also the same for glycerol（P < 0.05）,and the expressions of PPARγ,FASNT,and HSL significantly up-regulated（P < 0.01）. Repressing miR-124 resulted in the reduction of lipid droplets and the significant decrease of TG,and the expressions of PPARγ and FASNT significantly down-regulated（P < 0.05）. In conclusion,miR-124 might regulate the differentiation of porcine adipocyte by suppressing TNF-α,laying a foundation for future studies on the mechanism of miR-124 regulating lipid metabolism.

Key words: miR-124, porcine, adipocyte differentiation, TNF-α

LI Hong-yi, LI Jia-biao, ZHENG Yi-lin, JI Lu-lin, ZHANG Mao, ZHENG En-qin. miR-124 Regulates Porcine Adipocyte Differentiation Trough Targeting TNF-α[J]. Biotechnology Bulletin, 2017, 33(3): 175-179.

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