Over-expression of MicroRNA-199a Intensifies the TNFα-induced Apoptosis of Adipocytes

doi:10.13560/j.cnki.biotech.bull.1985.2017.03.026

Abstract

Abstract: The aim of this study is to explore the effects of MicroRNA-199a（miR-199a）on adipocytes apoptosis and the role of nuclear factor κB（NF-κB）. Tumor necrosis factor α（TNFα）was used to induce the apoptosis of cultured 3T3-L1 adipocytes. Based on this work,adding PDTC of NF-κB blocker and miR-199a mimic,the effect of overexpression of miR-199a on TNFα-induced apoptosis and the role of NF-κB in it were determined. The flow cytometry was used to analyze the cell apoptosis,kit Caspase3/7 to detect the enzymatic activities,dual luciferase reporter assay to detect the activity of NF-κB,qRT-PCR to detect the expression of miR-199a,and Western blotting to detect the changes of relevant proteins. As results showed,TNFα induced the apoptosis of differentiated adipocytes,concurrently activated NF-κB. The activated NF-κB played a negative regulator role in the apoptosis of adipocytes mediated by TNFα. Over-expressed miR-199a significantly suppressed the activation of NF-κB,and then remarkably promoted the apoptosis induced by TNFα. Conclusively,miR-199a involves in the TNFα-induced apoptosis of adipocytes through regulating NF-κB activation.

Key words: miR-199a, adipocyte, apoptosis, TNFα, NF-κB

QI Ren-li, WANG Qi, WU Yong-jiang, WANG Jing, HUANG Jin-xiu, YANG Fei-yun. Over-expression of MicroRNA-199a Intensifies the TNFα-induced Apoptosis of Adipocytes[J]. Biotechnology Bulletin, 2017, 33(3): 180-185.

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