Biotechnology Bulletin ›› 2021, Vol. 37 ›› Issue (1): 145-154.doi: 10.13560/j.cnki.biotech.bull.1985.2020-0396
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ZHU Qing-yuan(), LI Tian-qing(
)
Received:
2020-04-09
Online:
2021-01-26
Published:
2021-01-15
Contact:
LI Tian-qing
E-mail:690720467@qq.com;litianqing12@sina.com
ZHU Qing-yuan, LI Tian-qing. Applications of Single-cell RNA Sequencing in Heart Development,Disease and Medicine[J]. Biotechnology Bulletin, 2021, 37(1): 145-154.
发表年份 | 测序技术 | UMI长度/bp | 基因数 | 精度 | 单细胞捕获方法 | 扩增噪音 | 单个细胞成本 | 参考文献 |
---|---|---|---|---|---|---|---|---|
2012 | Smart-seq/C1 | 0 | 中 | 中 | 平板 | 高 | 高 | [9] |
2014 | Smart-seq2 | 0 | 高 | 高 | 平板 | 中高 | 高 | [11] |
2014 | MARS-seq | 8 | 低 | 低 | 微流 | 中 | 高 | [12] |
2015 | Drop-seq | 8 | 低 | 中高 | 液滴 | 低 | 中 | [14] |
2016 | CEL-seq2/C1 | 6 | 中 | 低 | 微流 | 低 | 高 | [20] |
2016 | SORT-seq | 4 | 高 | 高 | 平板 | 低 | 中 | [21] |
2017 | 10×Genomics | 10 | 高 | 中 | 油滴 | 低 | 低 | [15] |
2018 | mcSCRB-seq | 10 | 中 | 中 | 平板 | 低 | 高 | [22] |
发表年份 | 测序技术 | UMI长度/bp | 基因数 | 精度 | 单细胞捕获方法 | 扩增噪音 | 单个细胞成本 | 参考文献 |
---|---|---|---|---|---|---|---|---|
2012 | Smart-seq/C1 | 0 | 中 | 中 | 平板 | 高 | 高 | [9] |
2014 | Smart-seq2 | 0 | 高 | 高 | 平板 | 中高 | 高 | [11] |
2014 | MARS-seq | 8 | 低 | 低 | 微流 | 中 | 高 | [12] |
2015 | Drop-seq | 8 | 低 | 中高 | 液滴 | 低 | 中 | [14] |
2016 | CEL-seq2/C1 | 6 | 中 | 低 | 微流 | 低 | 高 | [20] |
2016 | SORT-seq | 4 | 高 | 高 | 平板 | 低 | 中 | [21] |
2017 | 10×Genomics | 10 | 高 | 中 | 油滴 | 低 | 低 | [15] |
2018 | mcSCRB-seq | 10 | 中 | 中 | 平板 | 低 | 高 | [22] |
细胞类型 | 共有标记基因 | 亚型 | 标记基因 | 结合空间转录组定位 |
---|---|---|---|---|
心肌 | TNNT2、TNNC1、ACTC1、TNNI3、ACTN2、NKX2-5、ENO3、COX6A2 | 心室肌 | MYH7、MYL2、LBH、NAV1、HAND1 | 心室 |
心房肌 | MYH6、TBX5、PAM、HNF4A | 心房 | ||
小梁肌 | ANGPT1、COL2A1、ITGA6、RELN、SLIT2、CRABP2 | |||
myoz2心肌 | MYOZ2、FABP3 | 心房和心室 | ||
表达细胞外基质基因心肌 | OPHN1、FOXK1、COL2A1、DCN | |||
致密化心肌 | ||||
内皮 | RAMP2、EMCN、PECAM1、CDH5、TIE1、TEK、HES1 | 冠状动脉内皮 | FABP4、CD36 | 致密化心肌 |
血管内皮 | ELN、FNLN5 | |||
瓣膜内皮 | NTRK2、NFATC1 | |||
心内膜内皮 | CDH11、NPR3 | 小梁肌 | ||
心外膜 | UPK3B、ALDH1A2、WT1、TBX18 | 心外膜 | CFB、C3、PRG4、ITLN1 | 心脏最外层包绕心脏 |
心外膜前体细胞 | KLK6、CRABP2、TCF21 | 房室区心外膜下间质 | ||
成纤维细胞 | DCN、COL1A1、FBLN1、LUM、TCF21 | 骨骼肌结缔组织样成纤维 | 流出道、瓣膜 | |
小血管发育成纤维 | 心外膜下 | |||
大血管发育成纤维 | 靠近流出道 | |||
平滑肌样成纤维 | 流出道、房室区心外膜下间质 | |||
神经嵴细胞 | ISL1、STMN2 | 纵隔间质和流出道 | ||
施旺细胞 | ALDH1A1、DHH | 纵隔间质和流出道房室外膜下间质 | ||
瓣膜细胞 | APCDD1、EDIL3、SCRG1、SLN、NR4A2 |
细胞类型 | 共有标记基因 | 亚型 | 标记基因 | 结合空间转录组定位 |
---|---|---|---|---|
心肌 | TNNT2、TNNC1、ACTC1、TNNI3、ACTN2、NKX2-5、ENO3、COX6A2 | 心室肌 | MYH7、MYL2、LBH、NAV1、HAND1 | 心室 |
心房肌 | MYH6、TBX5、PAM、HNF4A | 心房 | ||
小梁肌 | ANGPT1、COL2A1、ITGA6、RELN、SLIT2、CRABP2 | |||
myoz2心肌 | MYOZ2、FABP3 | 心房和心室 | ||
表达细胞外基质基因心肌 | OPHN1、FOXK1、COL2A1、DCN | |||
致密化心肌 | ||||
内皮 | RAMP2、EMCN、PECAM1、CDH5、TIE1、TEK、HES1 | 冠状动脉内皮 | FABP4、CD36 | 致密化心肌 |
血管内皮 | ELN、FNLN5 | |||
瓣膜内皮 | NTRK2、NFATC1 | |||
心内膜内皮 | CDH11、NPR3 | 小梁肌 | ||
心外膜 | UPK3B、ALDH1A2、WT1、TBX18 | 心外膜 | CFB、C3、PRG4、ITLN1 | 心脏最外层包绕心脏 |
心外膜前体细胞 | KLK6、CRABP2、TCF21 | 房室区心外膜下间质 | ||
成纤维细胞 | DCN、COL1A1、FBLN1、LUM、TCF21 | 骨骼肌结缔组织样成纤维 | 流出道、瓣膜 | |
小血管发育成纤维 | 心外膜下 | |||
大血管发育成纤维 | 靠近流出道 | |||
平滑肌样成纤维 | 流出道、房室区心外膜下间质 | |||
神经嵴细胞 | ISL1、STMN2 | 纵隔间质和流出道 | ||
施旺细胞 | ALDH1A1、DHH | 纵隔间质和流出道房室外膜下间质 | ||
瓣膜细胞 | APCDD1、EDIL3、SCRG1、SLN、NR4A2 |
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