Application of Single-cell Transcriptome Sequencing in Mammalian

doi:10.13560/j.cnki.biotech.bull.1985.2022-0094

Abstract

Abstract:

Mammalian organs are composed of many cell types. They send signals through cell-cell interactions to maintain homeostasis and ensure body development. Traditional transcriptome sequencing takes a large number of cells or tissues as research samples，reflecting the overall transcriptome characteristics of cells，but cannot analyze the gene expression of a single cell. The development of single cell RNA sequencing（scRNA-seq）provides an effective method for revealing the characteristics of single cell transcriptome. In this paper，the scRNA-seq platform，the main types of scRNA-seq technologies and the applications of scRNA-seq in mammals are reviewed.

Key words: single cell transcriptome sequencing, mammalian, cell types, heterogeneity

KOU Jia-yi, WANG Yu-ling, ZENG Rui-lin, LAN Dao-liang. Application of Single-cell Transcriptome Sequencing in Mammalian[J]. Biotechnology Bulletin, 2022, 38(11): 41-48.

Figures/Tables 1

References 73

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Items	10x Genomics	BD Rhapsody^TM
单细胞分离	微流控芯片，油包水	蜂巢板（scanner检测）
通量	同时测8个样本	最多可测4个样本
细胞活性	85%以上	65%以上
细胞直径	小于40 μm	小于40 μm
表面蛋白检测	可以	可以
Single Cell ATAC-SEQ	可以	不能

Items	10x Genomics	BD Rhapsody^TM
单细胞分离	微流控芯片，油包水	蜂巢板（scanner检测）
通量	同时测8个样本	最多可测4个样本
细胞活性	85%以上	65%以上
细胞直径	小于40 μm	小于40 μm
表面蛋白检测	可以	可以
Single Cell ATAC-SEQ	可以	不能