生物技术通报 ›› 2014, Vol. 0 ›› Issue (12): 153-160.doi: 10.13560/j.cnki.biotech.bull.1985.2014.12.025

• 研究报告 • 上一篇    下一篇

核桃青皮废弃物中木霉菌的分离及其适应性研究

杨卫民, 冉翠香, 高兴盛 ,师亚波, 赵林芳   

  1. 吕梁学院生命科学系,吕梁 033001
  • 收稿日期:2014-03-12 出版日期:2014-12-08 发布日期:2014-12-12
  • 作者简介:杨卫民,男,教授,研究方向:植物资源可持续开发与利用;E-mail:yangweimin0318@sina.com
  • 基金资助:
    山西省教育厅高校科技研究开发项目和高新技术产业化项目(201206),山西省高等学校大学生创新创业训练项目(2013380), 吕梁学院自然科学校内基金项目(ZRXN201311),吕梁学院校级大学生创新创业训练项目(CXCYZD201312)

Research on Isolation of Trichoderma from Waste of Walnut Peel and Adaptability

Yang Weimin, Ran Cuixiang, Gao Xingsheng, Shi Yabo, Zhao Linfang   

  1. (Department of Life Science, Lüliang University, Lüliang 033001)
  • Received:2014-03-12 Published:2014-12-08 Online:2014-12-12

摘要: 核桃果实采摘及加工过程中产生有毒有害物质的青皮遗弃物问题已经引起社会的关注。以核桃青皮为原料,湿热 灭菌(温度 121℃,时间 30 min)后让其自然霉变,从中分离出滋生菌株,结合反证试验获得优势菌种。并通过检测霉变前后青 皮中主要营养物质含量的变化对该菌种的适应性等进行研究。结果显示,从核桃青皮中分离出 4 株菌株,经鉴定分别为黄曲霉(Aspergillus flavus)、黑曲霉(Aspergillus niger)、青霉(Penicillium)和木霉(Trichoderma spp.),其中木霉属菌株是优势菌种。霉变 前后青皮营养物质变化情况为可溶性糖、脂肪、蛋白质和纤维素含量分别减少了 6.03%、2.97%、7.68% 和 64.7%。木霉菌对核桃青皮有良好的适应性和分解效果,可开发为生物肥料和新型生物杀菌剂。

关键词: 核桃青皮, 优势菌种, 木霉菌, 生物杀菌剂

Abstract: The peel derelict of hazardous substances from walnut fruit picking and processing has given a rise to the concern around the world. Walnut peel was used as the raw material and was left to mildew after its moist heat sterilization(temperature 121℃, 30 min)treatment, and then breeding strains were isolated from the walnut peel. The dominant species were to be gained through disproveal experiments, and adaptability of the species through detection changes in the main nutrient content of the peel around the mildew was researched . The results showed that the four walnut strains were isolated, which fall into Aspergillus flavus, Aspergillus niger, Penicillium and Trichoderma. Trichoderma strains is the most dominant strain among all the strains. The change of peel nutrients after mildew as follows :soluble sugar content decreases by 6.03%, fat content decreases by2.97% ;proteins decreases by 7.68%, cellulose content decreases by 64.7%. In a conclusion, Trichoderma is adaptable to walnut peel and has decomposition effects, and can be developed as a new bio-fertilizers and biocides.

Key words: Walnut peel, Dominant strain, Trichoderma, Biological fungicide