水稻OsSHAT1和OsRSR1应答激素及逆境胁迫的表达特性

doi:10.13560/j.cnki.biotech.bull.1985.2015.04.012

摘要/Abstract

摘要： 旨在研究AP2家族类因子是否参与逆境胁迫应答过程。通过序列比对发现，水稻OsSHAT1和OsRSR1蛋白序列一致性达41.92%，表明OsSHAT1和OsRSR1是同源性较高的AP2家族因子；且OsSHAT1和OsRSR1基因启动子序列都包含有ABRE、DRE、MYB、MYC、WRKY、GCC-box和ERE等应答不同胁迫及激素信号的元件。基因表达分析表明，OsSHAT1受低温、NaCl、ABA、ACC抑制，受干旱诱导表达；而OsRSR1受低温、干旱、ABA、ACC抑制，受NaCl诱导表达。推测OsSHAT1和OsRSR1可能通过不同的转录调控方式影响耐逆相关基因的表达，进而调节水稻不同的逆境胁迫反应。

关键词: AP2, OsSHAT1, OsRSR1, 非生物胁迫, 水稻

Abstract: It was to research whether AP2 family factors involved in the stress response process. In this study, sequence alignment revealed that OsSHAT1 and OsRSR1 proteins share 41.92% sequence identity, indicating that OsSHAT1 and OsRSR1 are homologous members of AP2 family. And the promoter sequences of OsSHAT1 and OsRSR1 genes contain multiple stress and hormone responsible cis-acting elements, such as ABRE, DRE, MYB, MYC, WRKY, GCC-box and ERE. Transcript analysis showed that the expression of OsSHAT1 was suppressed by low temperature, NaCl, ABA, ACC, but induced by drought；while the transcripts of OsRSR1 was inhibited by low temperature, drought, ABA and ACC, but enhanced by NaCl. It is speculated that OsSHAT1 and OsRSR1 may influence the expression of stress related genes by transcriptional regulation in differential ways, and then adjust the distinctive stress response of rice.

Key words: AP2, OsSHAT1, OsRSR1, abiotic stress, rice

李哲, 张少绚, 黄荣峰. 水稻OsSHAT1和OsRSR1应答激素及逆境胁迫的表达特性[J]. 生物技术通报, 2015, 31(3): 88-95.

Li Zhe, Zhang Shaoxuan, Huang Rongfeng,. The Expression of Rice OsSHAT1 and OsRSR1 in Response to Hormones and Abiotic Stresses[J]. Biotechnology Bulletin, 2015, 31(3): 88-95.

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https://biotech.aiijournal.com/CN/Y2015/V31/I3/88

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水稻OsSHAT1和OsRSR1应答激素及逆境胁迫的表达特性

The Expression of Rice OsSHAT1 and OsRSR1 in Response to Hormones and Abiotic Stresses

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[1]	王子颖, 龙晨洁, 范兆宇, 张蕾. 利用酵母双杂交系统筛选水稻中与OsCRK5互作蛋白[J]. 生物技术通报, 2023, 39(9): 117-125.
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[3]	李雪琪, 张素杰, 于曼, 黄金光, 周焕斌. 基于CRISPR/CasX介导的水稻基因组编辑技术的建立[J]. 生物技术通报, 2023, 39(9): 40-48.
[4]	吴元明, 林佳怡, 柳雨汐, 李丹婷, 张宗琼, 郑晓明, 逄洪波. 基于BSA-seq和RNA-seq挖掘水稻株高相关QTL[J]. 生物技术通报, 2023, 39(8): 173-184.
[5]	姚莎莎, 王晶晶, 王俊杰, 梁卫红. 植物激素信号通路调控水稻粒型的分子机制[J]. 生物技术通报, 2023, 39(8): 80-90.
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[7]	赵雪婷, 高利燕, 王俊刚, 沈庆庆, 张树珍, 李富生. 甘蔗AP2/ERF转录因子基因ShERF3的克隆、表达及其编码蛋白的定位[J]. 生物技术通报, 2023, 39(6): 208-216.
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[9]	冯珊珊, 王璐, 周益, 王幼平, 方玉洁. WOX家族基因调控植物生长发育和非生物胁迫响应的研究进展[J]. 生物技术通报, 2023, 39(5): 1-13.
[10]	翟莹, 李铭杨, 张军, 赵旭, 于海伟, 李珊珊, 赵艳, 张梅娟, 孙天国. 异源表达大豆转录因子GmNF-YA19提高转基因烟草抗旱性[J]. 生物技术通报, 2023, 39(5): 224-232.
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