微量热泳动技术原理及其在研究生物分子互作方面的应用

doi:10.13560/j.cnki.biotech.bull.1985.2015.06.010

生物技术通报 ›› 2015, Vol. 31 ›› Issue (6): 67-73.doi: 10.13560/j.cnki.biotech.bull.1985.2015.06.010

微量热泳动技术原理及其在研究生物分子互作方面的应用

艾秋实^1，2，3 曹向宇^1，3 赵芊^1，3 牛亚利^1，3，4 宋水山^1，3

1. 河北省科学院生物研究所，石家庄 050081；2. 河北农业大学生命科学学院，保定 071001；3.河北省主要农作物病害微生物控制工程技术研究中心，石家庄 050081；4.河北工业大学化工学院，天津 300130

收稿日期:2014-08-21 出版日期:2015-06-19 发布日期:2015-06-20
作者简介:艾秋实，男，硕士研究生，研究方向：植物抗逆机理；E-mail：yingyangtt@163.com
基金资助:
国家自然科学基金项目(31270880)

Principle and Application of Microscale Thermophoresis in Studies of Biomolecular Interactions

Ai Qiushi^1，2，3, Cao Xiangyu^1，3, Zhao Qian^1，3, Niu Yali^1，3，4, Song Shuishan^1，3,

1. Biology Institute，Hebei Academy of Sciences，Shijiazhuang 050081；2. College of Life Sciences，Agricultural University of Hebei，Baoding 071001；3. Hebei Engineering and Technology Center of Microbiological Control on Main Crop Disease，Shijiazhuang 050081；4. College of Chemistry and Engineering，Hebei University of Technology，Tianjin 300130

Received:2014-08-21 Published:2015-06-19 Online:2015-06-20

摘要/Abstract

摘要： 微量热泳动(MST)技术是近年来兴起的一项用于研究生物分子间互作的新技术。其检测是基于热泳动现象，即分子在温度梯度中的定向运动及由此引起分子性质的变化，如分子大小、电荷和水化层及构象等。该方法把精确的荧光检测与灵敏的热泳动相结合，从而提供了一个灵敏的、快速的精确分析生物分子间互作的检测方法。就MST的工作原理和检测过程及其在生物学研究中的应用作以综述。

关键词: 微量热泳动, 互作分析, 结合性研究, 生物分子间互作

Abstract: Microscale Thermophoresis(MST)is a new technique to study biomolecular interactions in recent years. It is based on thermophoresis, i.e., the directional movements of molecules in a temperature gradient, which results in subsequent changes of molecular properties such as sizes, charges, hydration shell and conformation. MST combines the precise fluorescence detection and sensitive thermophoresis and provides a sensitive, fast and precise detection technique to analyze biomolecular interactions. Working principle, detection process and application of MST in biology researches are reviewed here.

Key words: microscale thermophoresis, interaction analysis, binding studies, biomolecular interaction

艾秋实, 曹向宇, 赵芊, 牛亚利, 宋水山. 微量热泳动技术原理及其在研究生物分子互作方面的应用[J]. 生物技术通报, 2015, 31(6): 67-73.

Ai Qiushi, Cao Xiangyu, Zhao Qian, Niu Yali, Song Shuishan,. Principle and Application of Microscale Thermophoresis in Studies of Biomolecular Interactions[J]. Biotechnology Bulletin, 2015, 31(6): 67-73.

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微量热泳动技术原理及其在研究生物分子互作方面的应用

Principle and Application of Microscale Thermophoresis in Studies of Biomolecular Interactions

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