水稻miR169o及其靶基因OsNF-YAs对缺水胁迫期表达模式

doi:10.13560/j.cnki.biotech.bull.1985.2015.08.034

生物技术通报 ›› 2015, Vol. 31 ›› Issue (8): 76-81.doi: 10.13560/j.cnki.biotech.bull.1985.2015.08.034

水稻miR169o及其靶基因OsNF-YAs对缺水胁迫期表达模式

陈禹彤, 陈华民, 余超, AmyThein, 田芳, 何晨阳

中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室,北京 100193

收稿日期:2015-03-09 出版日期:2015-08-21 发布日期:2015-08-22
作者简介:陈禹彤,女,硕士研究生,研究方向：分子植物病理学;E-mail：cythxmichelle@163.com
基金资助:
国家转基因生物新品种培育重大专项（2014ZX08010-005,2014ZX08001-002）,国家“863”计划（2012AA101504）

Dynamic Expression of miR169o and Its Target Genes OsNF-YAs in the Early Response to Water Deficiency in Rice

Chen Yutong, Chen Huamin, Yu Chao, Amy Thein, Tian Fang, He Chenyang

State Key Laboratory for Biology of Plant Disease and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Science,Beijing 100193

Received:2015-03-09 Published:2015-08-21 Online:2015-08-22

摘要/Abstract

摘要： MicroRNAs（miRNAs）是一类小的非编码RNA,在植物逆境胁迫应答中发挥重要的调控作用。miR169可受干旱胁迫诱导表达,而过表达miR169则可以增强植物对干旱的耐受性。然而,miR169及其靶基因NF-YAs在水稻干旱胁迫条件下的表达动态至今尚不清楚。对水稻进行不同时间缺水处理,用qRT-PCR法定量测定了水稻根、茎、叶组织中miR169o及其靶基因表达的动态变化。结果表明,随着缺水处理时间的增加,水稻不同组织中miR169o表达量总体上有升高趋势;而靶基因（NF-YA1、NF-YA2 和NF-YA3）表达模式基本与miR169o的表达模式相反,但并不全部对应。推测在水稻干旱胁迫早期反应中,miR169o可能主要调控了部分特定靶基因的表达。此外,miR169o在水稻根、茎、叶组织中的表达和丰度存在着明显的差异,具有组织特异性。

关键词: miR169o, OsNF-YAs, 水稻, 干旱胁迫, 表达模式

Abstract: MicroRNAs（miRNAs）is noncoding RNAs and play important roles in plant development and response to various environment stresses. Recent evidence have indicated that miR169 is upregulated response to drought stress, and overexpression of miR169 contributes to improve the tolerance to drought stress in plant. However, it is still unclear to the expression pattern of miR169 under drought stress and the regulatory mechanism response to drought stress in rice. In this study, the dynamic expression patterns of miR169o and its target gene NF-YAs（Nuclear Factor Y A subunit, NF-YA）in the root, stem and leaf of rice were analysed systematically by qRT-PCR after water deficiency treatment. Generally, miR169o showed up-regulated after water deficiency stress treatment compared to that before treatment, while target gene NF-YAs showed imperfect reverse expression pattern to miR169o. In addition, the expression pattern and abundance of miR169o in rice root, stem and leaf suggested that the expression of miR169o is tissue-specific.

Key words: miR169o, OsNF-YAs, rice, water deficiency, expression pattern

陈禹彤, 陈华民, 余超, AmyThein, 田芳, 何晨阳. 水稻miR169o及其靶基因OsNF-YAs对缺水胁迫期表达模式[J]. 生物技术通报, 2015, 31(8): 76-81.

Chen Yutong, Chen Huamin, Yu Chao, Amy Thein, Tian Fang, He Chenyang. Dynamic Expression of miR169o and Its Target Genes OsNF-YAs in the Early Response to Water Deficiency in Rice[J]. Biotechnology Bulletin, 2015, 31(8): 76-81.

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水稻miR169o及其靶基因OsNF-YAs对缺水胁迫期表达模式

Dynamic Expression of miR169o and Its Target Genes OsNF-YAs in the Early Response to Water Deficiency in Rice

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