甘蔗工艺成熟期SS和SPS酶活性与糖分积累的相关性研究

doi:10.13560/j.cnki.biotech.bull.1985.2015.09.014

生物技术通报 ›› 2015, Vol. 31 ›› Issue (9): 105-110.doi: 10.13560/j.cnki.biotech.bull.1985.2015.09.014

甘蔗工艺成熟期SS和SPS酶活性与糖分积累的相关性研究

牛俊奇¹, 黄静丽¹, 赵文慧¹, 杨丽涛^1，2, 李杨瑞^1，2

(1.广西大学农学院亚热带农业生物资源保护与利用国家重点实验室，南宁 530005；2.中国农业科学院甘蔗研究中心广西农业科学院甘蔗研究所农业部广西甘蔗生物技术与遗传改良重点实验室广西甘蔗遗传改良重点实验室，南宁 530007)

收稿日期:2015-01-16 出版日期:2015-09-15 发布日期:2015-09-16
作者简介:牛俊奇，男，博士研究生，研究方向：甘蔗生理和分子生物学；E-mail：niujunqi3218@163.com
基金资助:
国家“863”计划课题(2013AA102604)，广西自然科学基金项目(2011GXNSFF018002，2013NXNSFAA019073)，国家国际合作项目(2009DFA30820，2013DFA31600)，广西八桂学者和特聘专家专项经费资助(2013)

Correlations Between Sucrose Accumulation and Activities of SPS, SS at Processing Maturing Stage of Sugarcane

Niu Junqi¹, Huang Jingli¹, Zhao Wenhui¹, Yang Litao^1，2, Li Yangrui^1，2

(1. Agricultural College，Guangxi University State Key Laboratory of Conservation and Utilization of Subtropical Agro-bioresources，Nanning 530005；2. Sugarcane Research Center，Chinese Academy of Agricultural Sciences Sugarcane Research Center，Guangxi Academy of Agricultural Sciences Key Laboratory of Sugarcane Biotechnology and Genetic Improvement(Guangxi)，Ministry of Agriculture Guangxi Key Laboratory of Sugarcane Genetic Improvement，Nanning 530007)

Received:2015-01-16 Published:2015-09-15 Online:2015-09-16

摘要/Abstract

摘要： 以工艺成熟期甘蔗品种GT28(早熟)和ROC22(早中熟)+1叶和不同节间为材料，分析SPS、SS合成和SS分解方向酶活性与节间糖分积累的相关性。结果表明，+1叶中SPS、SS合成和分解方向的酶活性比蔗茎中高，说明+1叶代谢活跃，是蔗茎糖分不断积累的物质基础。节间SPS酶活性与节间锤度、可溶性总糖和蔗糖含量都正相关，GT28节间SPS酶活性比ROC22中高。节间SS合成方向酶活性与锤度和蔗糖含量都负相关，GT28中SS合成方向酶活性比ROC22中低。而SS分解方向酶活性与ROC22节间锤度、可溶性糖和蔗糖含量都呈负相关，与GT28中呈正相关，未达到显著水平。说明成熟期节间SPS和SS合成方向酶活性提高有利于蔗糖的积累，而随着节间成熟，蔗糖含量可能是促使SS酶活性由合成方向向分解方向转化的一个重要调节因子。

关键词: 甘蔗, 蔗糖磷酸合成酶, 蔗糖合成酶, 蔗糖含量, 相关性分析

Abstract: The correlations between sucrose accumulation and activities of 3 enzymes viz, sucrose phosphate synthase(SPS), sucrose synthase in the synthesis direction(SS-s), and sucrose synthase in the cleavage direction(SS-c)were studied using the leaf+1 and 7 internodes of early-maturing cultivar GT28 and early-intermediate-maturing cultivar ROC22 at processing maturing stage of sugarcane. The results indicated that the activities of SPS, SS-s and SS-c in the leaf+1 were higher than those in stalks of both GT28 and ROC22, suggesting that the leaf+1 was vigorous and active for physiological metabolism, which was the substantial basis for continuous sucrose accumulation in stalks. The SPS activity in internode was significantly and positively correlated with the brix, total soluble sugar and sucrose content of internode, and it was higher in GT28 than in ROC22. Meanwhile, SS-s activity was significantly and negatively correlated with the brix and sucrose content in internode, and it was lower in GT28 than in ROC22. However, SS-c was significantly and negatively correlated with brix, total soluble sugar and sucrose content in the internode of ROC22, while positively in GT28 although it was not significant statistically. This suggests that SPS and SS-s activities promote the sucrose accumulation in the immature internodes；with the internode maturing, sucrose content might be an important regulatory factor to convert the SS activity from synthesis to cleavage direction.

Key words: sugarcane, sucrose phosphate synthase, sucrose synthase, sucrose accumulation, correlation analysis

牛俊奇, 黄静丽, 赵文慧, 杨丽涛, 李杨瑞. 甘蔗工艺成熟期SS和SPS酶活性与糖分积累的相关性研究[J]. 生物技术通报, 2015, 31(9): 105-110.

Niu Junqi, Huang Jingli, Zhao Wenhui, Yang Litao, Li Yangrui. Correlations Between Sucrose Accumulation and Activities of SPS, SS at Processing Maturing Stage of Sugarcane[J]. Biotechnology Bulletin, 2015, 31(9): 105-110.

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甘蔗工艺成熟期SS和SPS酶活性与糖分积累的相关性研究

Correlations Between Sucrose Accumulation and Activities of SPS, SS at Processing Maturing Stage of Sugarcane

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