马铃薯疮痂病拮抗菌的筛选鉴定及防治效果初探

doi:10.13560/j.cnki.biotech.bull.1985.2018-0417

生物技术通报 ›› 2018, Vol. 34 ›› Issue (10): 116-121.doi: 10.13560/j.cnki.biotech.bull.1985.2018-0417

马铃薯疮痂病拮抗菌的筛选鉴定及防治效果初探

李玉聪¹, 李滨影¹, 油心怡¹, 刘宇豪¹, 周波¹, 林榕姗^{1, 2}

1. 山东农业大学,泰安 271018;
2. 农业部农业微生物资源收集与保藏重点实验室,北京 100081

收稿日期:2018-05-04 出版日期:2018-10-26 发布日期:2018-11-07
作者简介:李玉聪,男,本科,研究方向：资源与环境微生物;E-mail：1104587028@qq.com
基金资助:
农业部农业微生物资源收集与保藏重点实验室开放基金项目（MRCP-2016-5）

Screening,Identification and Biocontrol Effect of Antagonistic Bacteria on Potato Common Scab

LI Yu-cong¹, Li Bin-ying¹, YOU Xin-yi¹, LIU Yu-hao¹, ZHOU Bo¹, LIN Rong-shan^{1, 2}

1. Shandong Agricultural University,Tai’an 271018;
2. Key Laboratory of Microbial Resources Collection and Preservation,Ministry of Agriculture,Beijing 100081

Received:2018-05-04 Published:2018-10-26 Online:2018-11-07

摘要/Abstract

摘要：

马铃薯疮痂病是马铃薯的主要病害之一,利用生物手段进行防治正逐渐引起人们的重视。为得到对马铃薯疮痂病病原菌具有较强拮抗作用的优良菌株,以病原菌Streptomyces bottropensis AMCC400023为靶标菌株,从山东省高密市马铃薯种植地采集的土壤中分离得到拮抗菌株,并采用平板对峙生长法和牛津杯试验法进行初筛和复筛,得到了1株具有较强拮抗作用的菌种1-3-4,其抑菌圈直径达19.74 mm,且其在盆栽试验中防治效果约为40.27%与48.66%。根据菌株1-3-4的16S rDNA序列分析结果,结合其培养形态特征和生理生化特性,将其鉴定为甲基营养型芽孢杆菌（Bacillus methylotrophicus）。盆栽防效测定试验结果显示,菌株1-3-4对于马铃薯疮痂病具有较好的防治作用,具有良好的应用前景。

关键词: 马铃薯疮痂病, 生物防治, 拮抗菌, 甲基营养型芽孢杆菌

Abstract:

Potato common scab is one of the major diseases of potato,and using biological means to control it gradually attracts researchers' attentions. In order to obtain a strain with strong antagonism to the pathogen of potato common scab,using Streptomyces bottropensis AMCC400023,a pathogen of potato common scab as a target strain,an antagonistic strain was isolated from the soil collected from the potato-planting area in Gaomi City of Shandong Province,and the plate confrontation growth method and the Oxford cup test were used for primary screening and rescreening. As results,a strain 1-3-4 with strong antagonistic effect was obtained,the diameter of the inhibition zone was 19.74 mm,and the control effect in pot experiment reached 40.27% and 48.66%. According to the 16S rDNA sequence analysis of strain 1-3-4,its culture morphological characteristics,as well as physiological and biochemical characteristics,it was identified as Bacillus methylotrophicus. The results of pots experiment showed that strain 1-3-4 had a promising control effect on potato common scab and had fine application prospects.

Key words: potato common scab, biological control, antagonistic bacteria, Bacillus methylotrophicus

李玉聪, 李滨影, 油心怡, 刘宇豪, 周波, 林榕姗. 马铃薯疮痂病拮抗菌的筛选鉴定及防治效果初探[J]. 生物技术通报, 2018, 34(10): 116-121.

LI Yu-cong, Li Bin-ying, YOU Xin-yi, LIU Yu-hao, ZHOU Bo, LIN Rong-shan. Screening,Identification and Biocontrol Effect of Antagonistic Bacteria on Potato Common Scab[J]. Biotechnology Bulletin, 2018, 34(10): 116-121.

参考文献

[1] Loria R, Bukhalid RA, Fry BA, et al.Plant pathogenicity in the genus Streptomyces[J]. Plant Disease, 1997, 81(8):836-846.
[2] Hiltunen LH, Kelloniemi J, Valkonen JPT.First report of Streptomyces europaeiscabiei causing common scab on potato in Finland[J]. Plant Disease, 2014, 98(9):1267-1267.
[3] Zhou B, Zhang MS, Ma XK.First Report of Streptomyces bottropensis causing potato common Scab in Hebei province, China[J]. Plant Disease, 2016, 101(3).
[4] Sarwar A, Latif Z, Cabaleiro C.First report of Streptomyces turgidiscabies causing potato common scab in Spain[J]. Plant Disease, 2017, 101(9).
[5] 赵伟全, 杨文香, 刘大群, 等. 中国马铃薯疮痂病研究初报[J]. 河北农业大学学报, 2004, 27(6):74-77.
[6] Hiltunen LH, Laakso I, Chobot V, et al.Influence of thaxtomins in different combinations and concentrations on growth of micropropagated potato shoot cultures[J]. Journal of Agricultural & Food Chemistry, 2006, 54(9):3372-3379.
[7] King RR, Lawrence CH, Clark MC.Correlation of phytotoxin production with pathogenicity of Streptomyces scabies isolates from scab infected potato tubers[J]. American Potato Journal, 1991, 68(10):675-680.
[8] Hiltunen LH, Weckman A, Ylhainen A, et al.Responses of potato cultivars to the common scab pathogens, Streptomyces scabies and S. turgidiscabies[J]. Annals of Applied Biology, 2015, 146(3):395-403.
[9] 布坎南. 伯杰. 细菌鉴定手册[M]. 北京:科学出版社, 1984.
[10] 蔡妙英, 东秀珠. 常见细菌系统鉴定手册[M]. 北京:科学出版社, 2001.
[11] 李勇, 郭凤柳, 赵伟全, 等. 马铃薯疮痂病菌拮抗菌ZWQ-1的鉴定及防效测定[J]. 河南农业科学, 2012, 41(10):94-99.
[12] Chen S, Zhang M, Wang J, et al.Biocontrol effects of Brevibacillus laterosporus AMCC100017 on potato common scab and its impact on rhizosphere bacterial communities[J]. Biological Control, 2017, 106:89-98.
[13] Liu DQ, Anderson NA, Kinkel LL.Field evaluation of antagonistic Streptormyces strains on biocontrol of potato scab[J]. Acta Phytopa Thologica Sinica, 2000, 30(3):237-244.
[14] Hiltunen LH, Ojanpera TH, Richter E, et al.Interactions and biocontrol of pathogenic Streptomyces strains co-occurring in potato scab lesions[J]. Journal of Applied Microbiology, 2009, 106(1):199.
[15] 李勇, 郭凤柳, 赵伟全, 等. 马铃薯疮痂病菌拮抗菌ZWQ-1的鉴定及防效测定[J]. 河南农业科学, 2012, 41(10):94-99.
[16] 徐红艳, 王卫, 王彬, 等. 刺五加内生放线菌对马铃薯疮痂病菌拮抗作用初探[J]. 西北农业学报, 2016, 25(11):1742-1748.
[17] 刘利强, 杨士玲, 陈强, 等. 30亿个/g甲基营养型芽孢杆菌可湿性粉剂防治黄瓜灰霉病田间药效试验[J]. 现代农业科技, 2014(9):130-130.
[18] 谢学文, 董瑞利, 石延霞, 等. 黄瓜炭疽病拮抗细菌的筛选及其抑制效果[J]. 中国生物防治学报, 2016, 32(2):215-220.
[19] 周登博, 陈宇丰, 井涛, 等. 抗香蕉枯萎病甲基营养型芽孢杆菌的鉴定及定殖[J]. 中国农学通报, 2017(36):145-151.

马铃薯疮痂病拮抗菌的筛选鉴定及防治效果初探

Screening,Identification and Biocontrol Effect of Antagonistic Bacteria on Potato Common Scab

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	褚睿, 李昭轩, 张学青, 杨东亚, 曹行行, 张雪艳. 黄瓜枯萎病拮抗芽孢杆菌的筛选、鉴定及其生防潜力[J]. 生物技术通报, 2023, 39(8): 262-271.
[2]	马俊秀, 吴皓琼, 姜威, 闫更轩, 胡基华, 张淑梅. 蔬菜软腐病菌广谱拮抗细菌菌株筛选鉴定及防效研究[J]. 生物技术通报, 2023, 39(7): 228-240.
[3]	任沛东, 彭健玲, 刘圣航, 姚姿婷, 朱桂宁, 陆光涛, 李瑞芳. 沙福芽孢杆菌GX-H6的分离鉴定及对水稻细菌性条斑病的防病效果[J]. 生物技术通报, 2023, 39(5): 243-253.
[4]	章乐乐, 王冠, 柳凤, 胡汉桥, 任磊. 芒果炭疽病拮抗菌分离、鉴定及生防机制研究[J]. 生物技术通报, 2023, 39(4): 277-287.
[5]	易希, 廖红东, 郑井元. 植物内生真菌防治根结线虫研究进展[J]. 生物技术通报, 2023, 39(3): 43-51.
[6]	王伟宸, 赵进, 黄薇颐, 郭芯竹, 李婉颖, 张卓. 芽胞杆菌代谢产物防治三种常见植物病原真菌的研究进展[J]. 生物技术通报, 2023, 39(3): 59-68.
[7]	杨东亚, 祁瑞雪, 李昭轩, 林薇, 马慧, 张雪艳. 黄瓜茄病镰刀菌拮抗芽孢杆菌的筛选、鉴定及促生效果[J]. 生物技术通报, 2023, 39(2): 211-220.
[8]	罗宁, 焦阳, 茆振川, 李惠霞, 谢丙炎. 木霉菌对根结线虫和孢囊线虫防治机理研究进展[J]. 生物技术通报, 2023, 39(2): 35-50.
[9]	马赛买, 李同源, 马燕军, 韩富军, 彭海, 孔维宝. 几丁质酶在农作物病虫害生物防治中的研究进展[J]. 生物技术通报, 2023, 39(10): 29-40.
[10]	祖雪, 周瑚, 朱华珺, 任佐华, 刘二明. 枯草芽孢杆菌K-268的分离鉴定及对水稻稻瘟病的防病效果[J]. 生物技术通报, 2022, 38(6): 136-146.
[11]	严聪文, 苏代发, 代庆忠, 张振荣, 田云霞, 董琼娥, 周文星, 陈杉艳, 童江云, 崔晓龙. 草莓病害的生物防治研究进展[J]. 生物技术通报, 2022, 38(12): 73-87.
[12]	舒洁, 张仁军, 梁应冲, 陈雅琼, 张娟, 郭建, 陈穗云. 植物源与微生物源生物制剂复配防治根结线虫病[J]. 生物技术通报, 2021, 37(7): 164-174.
[13]	张洁, 夏明聪, 朱文倩, 梁娟, 孙润红, 徐文, 武超, 杨丽荣. 蔬菜根结线虫生防芽胞杆菌的筛选及作用机理研究[J]. 生物技术通报, 2021, 37(7): 175-182.
[14]	陈立杰, 杨帆, 范海燕, 赵迪, 王媛媛, 朱晓峰, 刘晓宇, 段玉玺. 非编码RNA在生防菌-植物线虫-寄主互作中的研究进展[J]. 生物技术通报, 2021, 37(7): 65-70.
[15]	李瑾, 彭可为, 潘求一, 朱哲远, 彭迪. 解淀粉芽胞杆菌HR-2的分离鉴定及对水稻稻瘟病菌的拮抗效果[J]. 生物技术通报, 2021, 37(3): 27-34.