白藜芦醇对IgA大鼠肾组织TH2型炎性因子及血清CIC的影响

doi:10.13560/j.cnki.biotech.bull.1985.2016-1065

摘要/Abstract

摘要： 旨在考察白藜芦醇对IgA大鼠肾组织IL-4、IL-5、IL-13三种TH2型炎性因子及血清循环免疫复合物（CIC）的影响。将10只正常大鼠作为正常组,另40只采用胎牛血清白蛋白（BGG）处理9周制备4组IgA肾病（IgAN）大鼠模型,9周末进行白藜芦醇给药,并根据给药剂量的不同分别定义为模型组（未给药）、低剂量组（15 g生药/kg）、中剂量组（30 g生药/kg）、高剂量组（45 g生药/kg）,12周末剖杀,采用免疫荧光染色检验造模有效性,采用RT-PCR检测肾组织IL-4、IL-5、IL-13表达水平,采用酶联免疫吸附法（ELISA法）测定血清CIC浓度。结果显示,造模成功;与正常组比较,模型组大鼠肾组织IL-4、IL-5、IL-13的相对表达水平极显著增高（P<0.001）,与模型组比较,低、中、高剂量组大鼠肾组织IL-4、IL-5、IL-13的相对表达水平均依次降低,各细胞因子指标组间两两比较均有统计学意义（P<0.01）;与正常组比较,模型组血清CIC浓度显著升高（P<0.01）,低、中、高剂量组均高于正常组,但与正常组比较均无统计学意义（P>0.05）,与模型组比较均显著更低（P<0.05）,并依次呈逐渐降低趋势。白藜芦醇可明显下调IgA大鼠肾组织总促炎性因子IL-5的表达,炎性反应减弱后抗炎性因子IL-4与IL-13也随之降低,同时白藜芦醇也能显著降低IgA大鼠血清CIC浓度。

关键词: 白藜芦醇, IgA肾病, TH2型炎性因子, 循环免疫复合物

Abstract: This work aims to investigate the effects of resveratrol on renal tissues of IL-4,IL-5 and IL-13 TH2 inflammatory factors and circulating immunocomplex（CIC）from serum of rats with IgA nephropathy. Ten normal rats were used as the normal group,and the other 40 rats were treated with fetal bovine gamma globulin（BGG）for 9 weeks to become 4 groups of IgA nephropathy（IgAN）rats. Resveratrol was administered to them at the end of the 9th week. According to the different dose of the drug,they were defined as model group（no drug）,low dose group（15 g crude drug/kg）,middle dose group（30 g crude drug/kg）,and high dose group（45 g crude drug/kg）. Then they were killed at the end of the 12th week,and the validity of the model was tested by immunofluorescence staining. The expression levels of IL-13,IL-5 and IL-4 renal tissues were detected by RT-PCR,and the CIC concentration of serum was measured by ELISA. Results showed that the experimental model was successful. In contrast to the normal group,the relative expression levels of IL-4,IL-5 and IL-13 in the model group were significantly higher（P < 0.001）. Compared with the model group,the relative expression levels of IL-4,IL-5,and IL-13 in the low,middle and high dose groups decreased gradually,and the comparison of the cytokine indexes between every two groups had statistical significance（P < 0.01）. Compared with the normal group,the CIC concentration from serum in the model group was significantly higher（P < 0.01）,and those in the low,medium and high dose groups were higher than the normal group,but there was no significant statistical significance when compared with the normal group（P > 0.05）;moreover,they showed a gradually decreasing trend in comparison with the model group and they were significantly lower（P < 0.05）. In summary,resveratrol significantly down-regulates the expressions of total pro-inflammatory factor IL-5 in the renal tissues of IgA rats,and the anti-inflammatory factors IL-4 and IL-13 decrease their values after the inflammatory reaction decreased. Besides,resveratrol also significantly reduce the CIC concentration of serum in IgA rats.

Key words: resveratrol, IgA nephropathy, TH2 inflammatory factor, circulating immunocomplex

周敏. 白藜芦醇对IgA大鼠肾组织TH2型炎性因子及血清CIC的影响[J]. 生物技术通报, 2017, 33(6): 128-133.

ZHOU Min. Effects of Resveratrol on Renal Tissue of TH2 Inflammatory Factors and CIC from Serum of Rats with IgA Nephropathy[J]. Biotechnology Bulletin, 2017, 33(6): 128-133.

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