上调基因-11(URG11)生物信息学与功能分析

doi:10.13560/j.cnki.biotech.bull.1985.2016.03.032

生物技术通报 ›› 2016, Vol. 32 ›› Issue (3): 203-208.doi: 10.13560/j.cnki.biotech.bull.1985.2016.03.032

上调基因-11(URG11)生物信息学与功能分析

姚杨,苏杰,刘凯歌,徐锐

西安医学院第一附属医院, 西安 710077

收稿日期:2015-06-04 出版日期:2016-03-24 发布日期:2016-03-25
作者简介:姚杨, 硕士研究生, 研究方向：生化与分子；E-mail：yaoyang1220@sina.com
基金资助:
西安市科技计划项目(SF1418(6)), 西安医学院科研项目(12FZ15), 西安医学院附属医院科研项目(XYFY11-14)

Function Analysis of Up-regulated Gene(URG11)Based on Its Bioinformatics

YAO Yang, SU Jie, LIU Kai-ge, Xu Rui

The First Affiliated Hospital of Xi'an Medical University, Xi’an 710077

Received:2015-06-04 Published:2016-03-24 Online:2016-03-25

摘要/Abstract

摘要： 应用基因芯片技术获取以稳定转染HBx基因的肝癌细胞HepG₂(HepG2-X)Y, 以及非转染的肝癌细胞HepG₂的差异表达基因, 利用生物信息学方法对其进行初步分析表明, 该蛋白基因编码673个氨基酸, 预测分子量为17.06 kD, 理论等电点为4.83, 定位于细胞核, 具有转录调控、生长因子、信号传导的功能, 同源性分析结果表明, 其碱基序列与已经报道的其他12个物种的相似率为76%-97%, 且符合种属之间的进化关系。

关键词: 乙肝相关性肝癌, 生物信息学分析, URG11, HBx

Abstract: This work was to obtain differentially-expressed genes with hepatoma cell HepG(HepG2-X)Y of stable transfected HBx and non-transfected hepatoma cell HepG₂using gene chip technology. The preliminary bioinformatic analysis demonstrated that the gene of the protein encoded 673 amino acids with a predicted molecular weight of 17.06 kD and pI 4.83. URG11 were mainly localized in the nuclear and played the role in transcription regulation, growth factor and signal transducer, and it showed 76%-97% identity with the others reported 12 species, as well as was consistent with the evolutionary relationship between species.

Key words: HBV-related hepatocellular carcinoma, bioinformatics, URG11, HBx

姚杨,苏杰,刘凯歌,徐锐. 上调基因-11(URG11)生物信息学与功能分析[J]. 生物技术通报, 2016, 32(3): 203-208.

YAO Yang, SU Jie, LIU Kai-ge, Xu Rui. Function Analysis of Up-regulated Gene(URG11)Based on Its Bioinformatics[J]. Biotechnology Bulletin, 2016, 32(3): 203-208.

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上调基因-11(URG11)生物信息学与功能分析

Function Analysis of Up-regulated Gene(URG11)Based on Its Bioinformatics

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